Novel Mitochondrial DNA Lineage Found among <i>Ochlerotatus communis</i> (De Geer, 1776) of the Nordic-Baltic Region
The <i>Ochlerotatus (Oc.) communis</i> complex consist of three Northern American species as well as a common Holarctic mosquito (Diptera: Culicidae) <i>Oc. communis</i> (De Geer, 1776). These sister species exhibit important ecological differences and are capable of transmit...
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MDPI AG
2020-06-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/11/6/397 |
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| author | Heli Kirik Lea Tummeleht Tobias Lilja Olavi Kurina |
| author_facet | Heli Kirik Lea Tummeleht Tobias Lilja Olavi Kurina |
| author_sort | Heli Kirik |
| collection | DOAJ |
| description | The <i>Ochlerotatus (Oc.) communis</i> complex consist of three Northern American species as well as a common Holarctic mosquito (Diptera: Culicidae) <i>Oc. communis</i> (De Geer, 1776). These sister species exhibit important ecological differences and are capable of transmitting various pathogens, but cannot always be differentiated by morphological traits. To investigate the <i>Oc. communis</i> complex in Europe, we compared three molecular markers (COI, ND5 and ITS2) from 54 Estonian mosquitoes as well as two COI marker sequences from Sweden. These sequences were subjected to phylogenetic analysis and screened for <i>Wolbachia</i> Hertig and Wolbach symbionts. Within and between groups, distances were calculated for each marker to better understand the relationships among individuals. Results demonstrate that a group of samples, extracted from adult female mosquitoes matching the morphology of <i>Oc. communis</i>, show a marked difference from the main species when comparing the mitochondrial markers COI and ND5. However, there is no variance between the same specimens when considering the nuclear ITS2. We conclude that <i>Oc. communis</i> encompasses two distinct mitochondrial DNA lineages in the Nordic-Baltic region. Further research is needed to investigate the origin and extent of these genetic differences. |
| first_indexed | 2024-03-10T18:52:46Z |
| format | Article |
| id | doaj.art-de345dc048884672b12d0ec07cfc88d6 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T18:52:46Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-de345dc048884672b12d0ec07cfc88d62023-11-20T05:03:13ZengMDPI AGInsects2075-44502020-06-0111639710.3390/insects11060397Novel Mitochondrial DNA Lineage Found among <i>Ochlerotatus communis</i> (De Geer, 1776) of the Nordic-Baltic RegionHeli Kirik0Lea Tummeleht1Tobias Lilja2Olavi Kurina3Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Friedrich Reinhold Kreutzwaldi 5D, 51006 Tartu, EstoniaInstitute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Friedrich Reinhold Kreutzwaldi 62, 51006 Tartu, EstoniaDepartment of Microbiology, Swedish National Veterinary Institute, 751 89 Uppsala, SwedenInstitute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Friedrich Reinhold Kreutzwaldi 5D, 51006 Tartu, EstoniaThe <i>Ochlerotatus (Oc.) communis</i> complex consist of three Northern American species as well as a common Holarctic mosquito (Diptera: Culicidae) <i>Oc. communis</i> (De Geer, 1776). These sister species exhibit important ecological differences and are capable of transmitting various pathogens, but cannot always be differentiated by morphological traits. To investigate the <i>Oc. communis</i> complex in Europe, we compared three molecular markers (COI, ND5 and ITS2) from 54 Estonian mosquitoes as well as two COI marker sequences from Sweden. These sequences were subjected to phylogenetic analysis and screened for <i>Wolbachia</i> Hertig and Wolbach symbionts. Within and between groups, distances were calculated for each marker to better understand the relationships among individuals. Results demonstrate that a group of samples, extracted from adult female mosquitoes matching the morphology of <i>Oc. communis</i>, show a marked difference from the main species when comparing the mitochondrial markers COI and ND5. However, there is no variance between the same specimens when considering the nuclear ITS2. We conclude that <i>Oc. communis</i> encompasses two distinct mitochondrial DNA lineages in the Nordic-Baltic region. Further research is needed to investigate the origin and extent of these genetic differences.https://www.mdpi.com/2075-4450/11/6/397<i>Ochlerotatus churchillensis</i><i>Ochlerotatus nevadensis</i><i>Ochlerotatus tahoensis</i>barcodingphylogeneticsspeciation |
| spellingShingle | Heli Kirik Lea Tummeleht Tobias Lilja Olavi Kurina Novel Mitochondrial DNA Lineage Found among <i>Ochlerotatus communis</i> (De Geer, 1776) of the Nordic-Baltic Region Insects <i>Ochlerotatus churchillensis</i> <i>Ochlerotatus nevadensis</i> <i>Ochlerotatus tahoensis</i> barcoding phylogenetics speciation |
| title | Novel Mitochondrial DNA Lineage Found among <i>Ochlerotatus communis</i> (De Geer, 1776) of the Nordic-Baltic Region |
| title_full | Novel Mitochondrial DNA Lineage Found among <i>Ochlerotatus communis</i> (De Geer, 1776) of the Nordic-Baltic Region |
| title_fullStr | Novel Mitochondrial DNA Lineage Found among <i>Ochlerotatus communis</i> (De Geer, 1776) of the Nordic-Baltic Region |
| title_full_unstemmed | Novel Mitochondrial DNA Lineage Found among <i>Ochlerotatus communis</i> (De Geer, 1776) of the Nordic-Baltic Region |
| title_short | Novel Mitochondrial DNA Lineage Found among <i>Ochlerotatus communis</i> (De Geer, 1776) of the Nordic-Baltic Region |
| title_sort | novel mitochondrial dna lineage found among i ochlerotatus communis i de geer 1776 of the nordic baltic region |
| topic | <i>Ochlerotatus churchillensis</i> <i>Ochlerotatus nevadensis</i> <i>Ochlerotatus tahoensis</i> barcoding phylogenetics speciation |
| url | https://www.mdpi.com/2075-4450/11/6/397 |
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