Simultaneous Quantification of 25 Fentanyl Derivatives and Metabolites in Oral Fluid by Means of Microextraction on Packed Sorbent and LC–HRMS/MS Analysis
Fentanyl and fentalogs’ intake as drugs of abuse is experiencing a great increase in recent years. For this reason, there are more and more cases in which it is important to recognize and quantify these molecules and related metabolites in biological matrices. Oral fluid (OF) is often used to find o...
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| Format: | Article |
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MDPI AG
2021-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/19/5870 |
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| author | Flaminia Vincenti Camilla Montesano Svetlana Pirau Adolfo Gregori Fabiana Di Rosa Roberta Curini Manuel Sergi |
| author_facet | Flaminia Vincenti Camilla Montesano Svetlana Pirau Adolfo Gregori Fabiana Di Rosa Roberta Curini Manuel Sergi |
| author_sort | Flaminia Vincenti |
| collection | DOAJ |
| description | Fentanyl and fentalogs’ intake as drugs of abuse is experiencing a great increase in recent years. For this reason, there are more and more cases in which it is important to recognize and quantify these molecules and related metabolites in biological matrices. Oral fluid (OF) is often used to find out if a subject has recently used a psychoactive substance and if, therefore, the person is still under the effect of psychotropics. Given its difficulty in handling, good sample preparation and the development of instrumental methods for analysis are essential. In this work, an analytical method is proposed for the simultaneous determination of 25 analytes, including fentanyl, several derivatives and metabolites. OF was collected by means of passive drool; sample pretreatment was developed in order to be fast, simple and possibly semi-automated by exploiting microextraction on packed sorbent (MEPS). The analysis was performed by means of LC–HRMS/MS obtaining good identification and quantification of all the analytes in less than 10 min. The proposed method was fully validated according to the Scientific Working Group for Forensic Toxicology (SWGTOX) international guidelines. Good results were obtained in terms of recoveries, matrix effect and sensitivity, showing that this method could represent a useful tool in forensic toxicology. The presented method was successfully applied to the analysis of proficiency test samples. |
| first_indexed | 2024-03-10T06:55:29Z |
| format | Article |
| id | doaj.art-de347717ccdb42eab5fd3bb247bf851d |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T06:55:29Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-de347717ccdb42eab5fd3bb247bf851d2023-11-22T16:33:55ZengMDPI AGMolecules1420-30492021-09-012619587010.3390/molecules26195870Simultaneous Quantification of 25 Fentanyl Derivatives and Metabolites in Oral Fluid by Means of Microextraction on Packed Sorbent and LC–HRMS/MS AnalysisFlaminia Vincenti0Camilla Montesano1Svetlana Pirau2Adolfo Gregori3Fabiana Di Rosa4Roberta Curini5Manuel Sergi6Department of Chemistry, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Chemistry, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Chemistry, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Scientific Investigation (RIS), Carabinieri, 00191 Rome, ItalyDepartment of Scientific Investigation (RIS), Carabinieri, 00191 Rome, ItalyDepartment of Chemistry, Sapienza University of Rome, 00185 Rome, ItalyFaculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, 64100 Teramo, ItalyFentanyl and fentalogs’ intake as drugs of abuse is experiencing a great increase in recent years. For this reason, there are more and more cases in which it is important to recognize and quantify these molecules and related metabolites in biological matrices. Oral fluid (OF) is often used to find out if a subject has recently used a psychoactive substance and if, therefore, the person is still under the effect of psychotropics. Given its difficulty in handling, good sample preparation and the development of instrumental methods for analysis are essential. In this work, an analytical method is proposed for the simultaneous determination of 25 analytes, including fentanyl, several derivatives and metabolites. OF was collected by means of passive drool; sample pretreatment was developed in order to be fast, simple and possibly semi-automated by exploiting microextraction on packed sorbent (MEPS). The analysis was performed by means of LC–HRMS/MS obtaining good identification and quantification of all the analytes in less than 10 min. The proposed method was fully validated according to the Scientific Working Group for Forensic Toxicology (SWGTOX) international guidelines. Good results were obtained in terms of recoveries, matrix effect and sensitivity, showing that this method could represent a useful tool in forensic toxicology. The presented method was successfully applied to the analysis of proficiency test samples.https://www.mdpi.com/1420-3049/26/19/5870fentanyloral fluidmicroextraction on packed sorbentLC–HRMS/MS |
| spellingShingle | Flaminia Vincenti Camilla Montesano Svetlana Pirau Adolfo Gregori Fabiana Di Rosa Roberta Curini Manuel Sergi Simultaneous Quantification of 25 Fentanyl Derivatives and Metabolites in Oral Fluid by Means of Microextraction on Packed Sorbent and LC–HRMS/MS Analysis Molecules fentanyl oral fluid microextraction on packed sorbent LC–HRMS/MS |
| title | Simultaneous Quantification of 25 Fentanyl Derivatives and Metabolites in Oral Fluid by Means of Microextraction on Packed Sorbent and LC–HRMS/MS Analysis |
| title_full | Simultaneous Quantification of 25 Fentanyl Derivatives and Metabolites in Oral Fluid by Means of Microextraction on Packed Sorbent and LC–HRMS/MS Analysis |
| title_fullStr | Simultaneous Quantification of 25 Fentanyl Derivatives and Metabolites in Oral Fluid by Means of Microextraction on Packed Sorbent and LC–HRMS/MS Analysis |
| title_full_unstemmed | Simultaneous Quantification of 25 Fentanyl Derivatives and Metabolites in Oral Fluid by Means of Microextraction on Packed Sorbent and LC–HRMS/MS Analysis |
| title_short | Simultaneous Quantification of 25 Fentanyl Derivatives and Metabolites in Oral Fluid by Means of Microextraction on Packed Sorbent and LC–HRMS/MS Analysis |
| title_sort | simultaneous quantification of 25 fentanyl derivatives and metabolites in oral fluid by means of microextraction on packed sorbent and lc hrms ms analysis |
| topic | fentanyl oral fluid microextraction on packed sorbent LC–HRMS/MS |
| url | https://www.mdpi.com/1420-3049/26/19/5870 |
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