Untargeted Metabolomics Reveals a Complex Impact on Different Metabolic Pathways in Scallop <i>Mimachlamys varia</i> (Linnaeus, 1758) after Short-Term Exposure to Copper at Environmental Dose
Ports are a good example of how coastal environments, gathering a set of diverse ecosystems, are subjected to pollution factors coming from human activities both on land and at sea. Among them, trace element as copper represents a major factor. Abundant in port ecosystem, copper is transported by ru...
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| Format: | Article |
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MDPI AG
2021-12-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/11/12/862 |
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| author | Vincent Hamani Pascaline Ory Pierre-Edouard Bodet Laurence Murillo Marianne Graber |
| author_facet | Vincent Hamani Pascaline Ory Pierre-Edouard Bodet Laurence Murillo Marianne Graber |
| author_sort | Vincent Hamani |
| collection | DOAJ |
| description | Ports are a good example of how coastal environments, gathering a set of diverse ecosystems, are subjected to pollution factors coming from human activities both on land and at sea. Among them, trace element as copper represents a major factor. Abundant in port ecosystem, copper is transported by runoff water and results from diverse port features (corrosion of structures, fuel, anti-fouling products, etc.). The variegated scallop <i>Mimachlamys varia</i> is common in the Atlantic port areas and is likely to be directly influenced by copper pollution, due to its sessile and filtering lifestyle. Thus, the aim of the present study is to investigate the disruption of the variegated scallop metabolism, under a short exposure (48 h) to a copper concentration frequently encountered in the waters of the largest marina in Europe (82 μg/L). For this, we chose a non-targeted metabolomic approach using ultra-high performance liquid chromatography coupled to high resolution mass spectrometry (UHPLC-HRMS), offering a high level of sensitivity and allowing the study without <i>a priori</i> of the entire metabolome. We described 28 metabolites clearly modulated by copper. They reflected the action of copper on several biological functions such as osmoregulation, oxidative stress, reproduction and energy metabolism. |
| first_indexed | 2024-03-10T03:35:38Z |
| format | Article |
| id | doaj.art-de34bf0a93bb4c78ab4ac8d491838da6 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T03:35:38Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-de34bf0a93bb4c78ab4ac8d491838da62023-11-23T09:31:58ZengMDPI AGMetabolites2218-19892021-12-01111286210.3390/metabo11120862Untargeted Metabolomics Reveals a Complex Impact on Different Metabolic Pathways in Scallop <i>Mimachlamys varia</i> (Linnaeus, 1758) after Short-Term Exposure to Copper at Environmental DoseVincent Hamani0Pascaline Ory1Pierre-Edouard Bodet2Laurence Murillo3Marianne Graber4UMR 7266 LIENSs, CNRS-La Rochelle Université, 2 Rue Olympe de Gouges, 17000 La Rochelle, FranceUMR 7266 LIENSs, CNRS-La Rochelle Université, 2 Rue Olympe de Gouges, 17000 La Rochelle, FranceUMR 7266 LIENSs, CNRS-La Rochelle Université, 2 Rue Olympe de Gouges, 17000 La Rochelle, FranceUMR 7266 LIENSs, CNRS-La Rochelle Université, 2 Rue Olympe de Gouges, 17000 La Rochelle, FranceUMR 7266 LIENSs, CNRS-La Rochelle Université, 2 Rue Olympe de Gouges, 17000 La Rochelle, FrancePorts are a good example of how coastal environments, gathering a set of diverse ecosystems, are subjected to pollution factors coming from human activities both on land and at sea. Among them, trace element as copper represents a major factor. Abundant in port ecosystem, copper is transported by runoff water and results from diverse port features (corrosion of structures, fuel, anti-fouling products, etc.). The variegated scallop <i>Mimachlamys varia</i> is common in the Atlantic port areas and is likely to be directly influenced by copper pollution, due to its sessile and filtering lifestyle. Thus, the aim of the present study is to investigate the disruption of the variegated scallop metabolism, under a short exposure (48 h) to a copper concentration frequently encountered in the waters of the largest marina in Europe (82 μg/L). For this, we chose a non-targeted metabolomic approach using ultra-high performance liquid chromatography coupled to high resolution mass spectrometry (UHPLC-HRMS), offering a high level of sensitivity and allowing the study without <i>a priori</i> of the entire metabolome. We described 28 metabolites clearly modulated by copper. They reflected the action of copper on several biological functions such as osmoregulation, oxidative stress, reproduction and energy metabolism.https://www.mdpi.com/2218-1989/11/12/862coppermetabolomicsUHPLC/QToF mass spectrometryscallop |
| spellingShingle | Vincent Hamani Pascaline Ory Pierre-Edouard Bodet Laurence Murillo Marianne Graber Untargeted Metabolomics Reveals a Complex Impact on Different Metabolic Pathways in Scallop <i>Mimachlamys varia</i> (Linnaeus, 1758) after Short-Term Exposure to Copper at Environmental Dose Metabolites copper metabolomics UHPLC/QToF mass spectrometry scallop |
| title | Untargeted Metabolomics Reveals a Complex Impact on Different Metabolic Pathways in Scallop <i>Mimachlamys varia</i> (Linnaeus, 1758) after Short-Term Exposure to Copper at Environmental Dose |
| title_full | Untargeted Metabolomics Reveals a Complex Impact on Different Metabolic Pathways in Scallop <i>Mimachlamys varia</i> (Linnaeus, 1758) after Short-Term Exposure to Copper at Environmental Dose |
| title_fullStr | Untargeted Metabolomics Reveals a Complex Impact on Different Metabolic Pathways in Scallop <i>Mimachlamys varia</i> (Linnaeus, 1758) after Short-Term Exposure to Copper at Environmental Dose |
| title_full_unstemmed | Untargeted Metabolomics Reveals a Complex Impact on Different Metabolic Pathways in Scallop <i>Mimachlamys varia</i> (Linnaeus, 1758) after Short-Term Exposure to Copper at Environmental Dose |
| title_short | Untargeted Metabolomics Reveals a Complex Impact on Different Metabolic Pathways in Scallop <i>Mimachlamys varia</i> (Linnaeus, 1758) after Short-Term Exposure to Copper at Environmental Dose |
| title_sort | untargeted metabolomics reveals a complex impact on different metabolic pathways in scallop i mimachlamys varia i linnaeus 1758 after short term exposure to copper at environmental dose |
| topic | copper metabolomics UHPLC/QToF mass spectrometry scallop |
| url | https://www.mdpi.com/2218-1989/11/12/862 |
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