Immobilization of Crude Polyphenol Oxidase Extracts from Apples on Polypyrrole as a Membrane for Phenol Removal
This research aims to make a polypyrrole (PPy) membrane and crude extract of polyphenol oxidase (PPO) as a membrane of mPPy/PPO apple extracts. The membrane of PPy/PPO-apple extract has been synthesized by the electrodeposition method. The electrolyte composition consists of a mixture of 0.10-0.20 M...
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Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University
2021-03-01
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| Series: | Jurnal Kimia Sains dan Aplikasi |
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| Online Access: | https://ejournal.undip.ac.id/index.php/ksa/article/view/33865 |
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| author | Anceu Murniati Buchari Buchari Suryo Gandasasmita Zeily Nurachman Arie Hardian Dera Triani |
| author_facet | Anceu Murniati Buchari Buchari Suryo Gandasasmita Zeily Nurachman Arie Hardian Dera Triani |
| author_sort | Anceu Murniati |
| collection | DOAJ |
| description | This research aims to make a polypyrrole (PPy) membrane and crude extract of polyphenol oxidase (PPO) as a membrane of mPPy/PPO apple extracts. The membrane of PPy/PPO-apple extract has been synthesized by the electrodeposition method. The electrolyte composition consists of a mixture of 0.10-0.20 M pyrrole (Py) and 50-100% PPO apple extract, which is stable using 50 mM of phosphate buffer solution at pH 6.80-7.00 and room temperature. The electrodeposition process is used 400 mesh steel gauze anode ST-304 and carbon plate cathode. Electrodeposition is carried out at potential = 5.00-6.00 V; current = 0.02-0.25 A; the distance from both electrodes = 1.00-2.00 cm for 300-500 seconds. The results from the deposition of PPy/PPO apple extract of the anode are a membrane of mPPy/PPO-apple extract, with total enzyme activity (U) = (957,1441, 2287 and 1754) using 2.00-5.00 mM phenol as a substrate which is measured based on the UV-visible spectrophotometric method. PPy and mPPy/PPO-apple extracts were characterized by SEM and SEM-EDS. The membrane of mPPy/PPO-apple extract can be used to remove phenol in industrial wastewater samples is 50-65% with a filtration capacity of 500 mL for 2 hours. |
| first_indexed | 2024-12-24T01:52:18Z |
| format | Article |
| id | doaj.art-de365fb89f964296a49a165395ad1a7f |
| institution | Directory Open Access Journal |
| issn | 1410-8917 2597-9914 |
| language | English |
| last_indexed | 2024-12-24T01:52:18Z |
| publishDate | 2021-03-01 |
| publisher | Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University |
| record_format | Article |
| series | Jurnal Kimia Sains dan Aplikasi |
| spelling | doaj.art-de365fb89f964296a49a165395ad1a7f2022-12-21T17:21:41ZengChemistry Department, Faculty of Sciences and Mathematics, Diponegoro UniversityJurnal Kimia Sains dan Aplikasi1410-89172597-99142021-03-01242626910.14710/jksa.24.2.62-6918234Immobilization of Crude Polyphenol Oxidase Extracts from Apples on Polypyrrole as a Membrane for Phenol RemovalAnceu Murniati0Buchari Buchari1Suryo Gandasasmita2Zeily Nurachman3Arie Hardian4https://orcid.org/0000-0002-8461-1444Dera Triani5Department of Chemistry, Faculty of Sciences and Informatics, Universitas Jenderal Achmad Yani, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, IndonesiaDepartment of Chemistry, Faculty of Sciences and Informatics, Universitas Jenderal Achmad Yani, IndonesiaDepartment of Chemistry, Faculty of Sciences and Informatics, Universitas Jenderal Achmad Yani, IndonesiaThis research aims to make a polypyrrole (PPy) membrane and crude extract of polyphenol oxidase (PPO) as a membrane of mPPy/PPO apple extracts. The membrane of PPy/PPO-apple extract has been synthesized by the electrodeposition method. The electrolyte composition consists of a mixture of 0.10-0.20 M pyrrole (Py) and 50-100% PPO apple extract, which is stable using 50 mM of phosphate buffer solution at pH 6.80-7.00 and room temperature. The electrodeposition process is used 400 mesh steel gauze anode ST-304 and carbon plate cathode. Electrodeposition is carried out at potential = 5.00-6.00 V; current = 0.02-0.25 A; the distance from both electrodes = 1.00-2.00 cm for 300-500 seconds. The results from the deposition of PPy/PPO apple extract of the anode are a membrane of mPPy/PPO-apple extract, with total enzyme activity (U) = (957,1441, 2287 and 1754) using 2.00-5.00 mM phenol as a substrate which is measured based on the UV-visible spectrophotometric method. PPy and mPPy/PPO-apple extracts were characterized by SEM and SEM-EDS. The membrane of mPPy/PPO-apple extract can be used to remove phenol in industrial wastewater samples is 50-65% with a filtration capacity of 500 mL for 2 hours.https://ejournal.undip.ac.id/index.php/ksa/article/view/33865mppy/ppo-apple extractphenolppo activitymembrane |
| spellingShingle | Anceu Murniati Buchari Buchari Suryo Gandasasmita Zeily Nurachman Arie Hardian Dera Triani Immobilization of Crude Polyphenol Oxidase Extracts from Apples on Polypyrrole as a Membrane for Phenol Removal Jurnal Kimia Sains dan Aplikasi mppy/ppo-apple extract phenol ppo activity membrane |
| title | Immobilization of Crude Polyphenol Oxidase Extracts from Apples on Polypyrrole as a Membrane for Phenol Removal |
| title_full | Immobilization of Crude Polyphenol Oxidase Extracts from Apples on Polypyrrole as a Membrane for Phenol Removal |
| title_fullStr | Immobilization of Crude Polyphenol Oxidase Extracts from Apples on Polypyrrole as a Membrane for Phenol Removal |
| title_full_unstemmed | Immobilization of Crude Polyphenol Oxidase Extracts from Apples on Polypyrrole as a Membrane for Phenol Removal |
| title_short | Immobilization of Crude Polyphenol Oxidase Extracts from Apples on Polypyrrole as a Membrane for Phenol Removal |
| title_sort | immobilization of crude polyphenol oxidase extracts from apples on polypyrrole as a membrane for phenol removal |
| topic | mppy/ppo-apple extract phenol ppo activity membrane |
| url | https://ejournal.undip.ac.id/index.php/ksa/article/view/33865 |
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