PluMu—A Mu-like Bacteriophage Infecting <i>Actinobacillus pleuropneumoniae</i>
<i>Actinobacillus pleuropneumoniae</i> is the causative agent of pleuropneumonia, an economically important lung disease in pigs. In draft genomes of two Cypriot clinical <i>A. pleuropneumoniae</i> isolates (MIDG3457 and MIDG3459), we previously identified single genomic regi...
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| Format: | Article |
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MDPI AG
2024-03-01
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| Series: | Applied Microbiology |
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| Online Access: | https://www.mdpi.com/2673-8007/4/1/37 |
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| author | Lee Julia Bartsch Roberto Fernandez Crespo Yunfei Wang Michael A. Skinner Andrew N. Rycroft William Cooley David J. Everest Yanwen Li Janine T. Bossé Paul R. Langford |
| author_facet | Lee Julia Bartsch Roberto Fernandez Crespo Yunfei Wang Michael A. Skinner Andrew N. Rycroft William Cooley David J. Everest Yanwen Li Janine T. Bossé Paul R. Langford |
| author_sort | Lee Julia Bartsch |
| collection | DOAJ |
| description | <i>Actinobacillus pleuropneumoniae</i> is the causative agent of pleuropneumonia, an economically important lung disease in pigs. In draft genomes of two Cypriot clinical <i>A. pleuropneumoniae</i> isolates (MIDG3457 and MIDG3459), we previously identified single genomic regions with homology to Mu-like bacteriophage and presented preliminary evidence of active phage. Here, updated Phastest genomic analysis identified two loci in both MIDG3457 and MIDG3459 that were predicted to encode proteins with high homology to, and whose organisation was characteristic of, Mu-like phages. Phylogenetically, the closest matches were with <i>Mannheimia</i> Vb and <i>Glaesserella</i> SuMu phages. Phastest scored the loci as “complete”, indicating they produced active phage. PCR amplification of the Mu-like phage c and <i>tail</i> genes from DNase-treated polyethylene glycol 8000 (PEG)-precipitated supernatants of MIDG3457 and MIDG3459 (grown in either Brain Heart Infusion-NAD or Grace’s Insect Medium-NAD broth) indicated the presence of intact virions. The phages from MIDG3457 and MIDG3459 were named PluMu 3457-1, 3457-2, and PluMu 3459-1 and PluMu 3459-2, respectively. Transmission electron microscopy (TEM) of the PEG-precipitated supernatants of broth-grown MIDG3459 identified virions with icosahedral heads and tails, consistent with other Mu-like phages. We conclude that MIDG3459 produces an active Mu-like phage. |
| first_indexed | 2024-04-24T18:36:06Z |
| format | Article |
| id | doaj.art-de37145f6b924ad5a59ad6efefc892e5 |
| institution | Directory Open Access Journal |
| issn | 2673-8007 |
| language | English |
| last_indexed | 2024-04-24T18:36:06Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Microbiology |
| spelling | doaj.art-de37145f6b924ad5a59ad6efefc892e52024-03-27T13:19:00ZengMDPI AGApplied Microbiology2673-80072024-03-014152053510.3390/applmicrobiol4010037PluMu—A Mu-like Bacteriophage Infecting <i>Actinobacillus pleuropneumoniae</i>Lee Julia Bartsch0Roberto Fernandez Crespo1Yunfei Wang2Michael A. Skinner3Andrew N. Rycroft4William Cooley5David J. Everest6Yanwen Li7Janine T. Bossé8Paul R. Langford9Section of Paediatric Infectious Disease, Imperial College London, St Mary’s Campus, London W2 1PG, UKSection of Paediatric Infectious Disease, Imperial College London, St Mary’s Campus, London W2 1PG, UKSection of Paediatric Infectious Disease, Imperial College London, St Mary’s Campus, London W2 1PG, UKSection of Virology, Imperial College London, St Mary’s Campus, London W2 1PG, UKPathobiology and Population Sciences, Royal Veterinary College, Hawkshead Campus, Hatfield AL9 7TA, UKDepartment of Pathology, Animal and Plant Health Agency, Addlestone KT15 3NB, UKDepartment of Pathology, Animal and Plant Health Agency, Addlestone KT15 3NB, UKSection of Paediatric Infectious Disease, Imperial College London, St Mary’s Campus, London W2 1PG, UKSection of Paediatric Infectious Disease, Imperial College London, St Mary’s Campus, London W2 1PG, UKSection of Paediatric Infectious Disease, Imperial College London, St Mary’s Campus, London W2 1PG, UK<i>Actinobacillus pleuropneumoniae</i> is the causative agent of pleuropneumonia, an economically important lung disease in pigs. In draft genomes of two Cypriot clinical <i>A. pleuropneumoniae</i> isolates (MIDG3457 and MIDG3459), we previously identified single genomic regions with homology to Mu-like bacteriophage and presented preliminary evidence of active phage. Here, updated Phastest genomic analysis identified two loci in both MIDG3457 and MIDG3459 that were predicted to encode proteins with high homology to, and whose organisation was characteristic of, Mu-like phages. Phylogenetically, the closest matches were with <i>Mannheimia</i> Vb and <i>Glaesserella</i> SuMu phages. Phastest scored the loci as “complete”, indicating they produced active phage. PCR amplification of the Mu-like phage c and <i>tail</i> genes from DNase-treated polyethylene glycol 8000 (PEG)-precipitated supernatants of MIDG3457 and MIDG3459 (grown in either Brain Heart Infusion-NAD or Grace’s Insect Medium-NAD broth) indicated the presence of intact virions. The phages from MIDG3457 and MIDG3459 were named PluMu 3457-1, 3457-2, and PluMu 3459-1 and PluMu 3459-2, respectively. Transmission electron microscopy (TEM) of the PEG-precipitated supernatants of broth-grown MIDG3459 identified virions with icosahedral heads and tails, consistent with other Mu-like phages. We conclude that MIDG3459 produces an active Mu-like phage.https://www.mdpi.com/2673-8007/4/1/37<i>Actinobacillus pleuropneumoniae</i>Mu-like bacteriophagepleuropneumoniavirulencePluMu<i>Pasteurellaceae</i> |
| spellingShingle | Lee Julia Bartsch Roberto Fernandez Crespo Yunfei Wang Michael A. Skinner Andrew N. Rycroft William Cooley David J. Everest Yanwen Li Janine T. Bossé Paul R. Langford PluMu—A Mu-like Bacteriophage Infecting <i>Actinobacillus pleuropneumoniae</i> Applied Microbiology <i>Actinobacillus pleuropneumoniae</i> Mu-like bacteriophage pleuropneumonia virulence PluMu <i>Pasteurellaceae</i> |
| title | PluMu—A Mu-like Bacteriophage Infecting <i>Actinobacillus pleuropneumoniae</i> |
| title_full | PluMu—A Mu-like Bacteriophage Infecting <i>Actinobacillus pleuropneumoniae</i> |
| title_fullStr | PluMu—A Mu-like Bacteriophage Infecting <i>Actinobacillus pleuropneumoniae</i> |
| title_full_unstemmed | PluMu—A Mu-like Bacteriophage Infecting <i>Actinobacillus pleuropneumoniae</i> |
| title_short | PluMu—A Mu-like Bacteriophage Infecting <i>Actinobacillus pleuropneumoniae</i> |
| title_sort | plumu a mu like bacteriophage infecting i actinobacillus pleuropneumoniae i |
| topic | <i>Actinobacillus pleuropneumoniae</i> Mu-like bacteriophage pleuropneumonia virulence PluMu <i>Pasteurellaceae</i> |
| url | https://www.mdpi.com/2673-8007/4/1/37 |
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