Metabolic and genomic analysis elucidates strain-level variation in Microbacterium spp. isolated from chromate contaminated sediment
Hexavalent chromium [Cr(VI)] is a soluble carcinogen that has caused widespread contamination of soil and water in many industrial nations. Bacteria have the potential to aid remediation as certain strains can catalyze the reduction of Cr(VI) to insoluble and less toxic Cr(III). Here, we examine Cr(...
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| Format: | Article |
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PeerJ Inc.
2015-11-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/1395.pdf |
| _version_ | 1797424652241338368 |
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| author | Michael W. Henson Jorge W. Santo Domingo Peter S. Kourtev Roderick V. Jensen James A. Dunn Deric R. Learman |
| author_facet | Michael W. Henson Jorge W. Santo Domingo Peter S. Kourtev Roderick V. Jensen James A. Dunn Deric R. Learman |
| author_sort | Michael W. Henson |
| collection | DOAJ |
| description | Hexavalent chromium [Cr(VI)] is a soluble carcinogen that has caused widespread contamination of soil and water in many industrial nations. Bacteria have the potential to aid remediation as certain strains can catalyze the reduction of Cr(VI) to insoluble and less toxic Cr(III). Here, we examine Cr(VI) reducing Microbacterium spp. (Cr-K1W, Cr-K20, Cr-K29, and Cr-K32) isolated from contaminated sediment (Seymore, Indiana) and show varying chromate responses despite the isolates’ phylogenetic similarity (i.e., identical 16S rRNA gene sequences). Detailed analysis identified differences based on genomic metabolic potential, growth and general metabolic capabilities, and capacity to resist and reduce Cr(VI). Taken together, the discrepancies between the isolates demonstrate the complexity inter-strain variation can have on microbial physiology and related biogeochemical processes. |
| first_indexed | 2024-03-09T08:04:53Z |
| format | Article |
| id | doaj.art-de3804405d7849f0b627f0e11a593969 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T08:04:53Z |
| publishDate | 2015-11-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-de3804405d7849f0b627f0e11a5939692023-12-03T00:25:47ZengPeerJ Inc.PeerJ2167-83592015-11-013e139510.7717/peerj.1395Metabolic and genomic analysis elucidates strain-level variation in Microbacterium spp. isolated from chromate contaminated sedimentMichael W. Henson0Jorge W. Santo Domingo1Peter S. Kourtev2Roderick V. Jensen3James A. Dunn4Deric R. Learman5Institute for Great Lakes Research and Department of Biology, Central Michigan University, Mount Pleasant, MI, United StatesNational Risk Management Research Laboratory, Environmental Protection Agency, Cincinnati, OH, USADepartment of Biology, Central Michigan University, Mount Pleasant, MI, United StatesDepartment of Biological Sciences, Virginia Polytechnic Institute and State University (Virginia Tech), Blacksburg, VA, United StatesInstitute for Great Lakes Research and Department of Biology, Central Michigan University, Mount Pleasant, MI, United StatesInstitute for Great Lakes Research and Department of Biology, Central Michigan University, Mount Pleasant, MI, United StatesHexavalent chromium [Cr(VI)] is a soluble carcinogen that has caused widespread contamination of soil and water in many industrial nations. Bacteria have the potential to aid remediation as certain strains can catalyze the reduction of Cr(VI) to insoluble and less toxic Cr(III). Here, we examine Cr(VI) reducing Microbacterium spp. (Cr-K1W, Cr-K20, Cr-K29, and Cr-K32) isolated from contaminated sediment (Seymore, Indiana) and show varying chromate responses despite the isolates’ phylogenetic similarity (i.e., identical 16S rRNA gene sequences). Detailed analysis identified differences based on genomic metabolic potential, growth and general metabolic capabilities, and capacity to resist and reduce Cr(VI). Taken together, the discrepancies between the isolates demonstrate the complexity inter-strain variation can have on microbial physiology and related biogeochemical processes.https://peerj.com/articles/1395.pdfMicrobacteriumChromium reductionStrain-level variation |
| spellingShingle | Michael W. Henson Jorge W. Santo Domingo Peter S. Kourtev Roderick V. Jensen James A. Dunn Deric R. Learman Metabolic and genomic analysis elucidates strain-level variation in Microbacterium spp. isolated from chromate contaminated sediment PeerJ Microbacterium Chromium reduction Strain-level variation |
| title | Metabolic and genomic analysis elucidates strain-level variation in Microbacterium spp. isolated from chromate contaminated sediment |
| title_full | Metabolic and genomic analysis elucidates strain-level variation in Microbacterium spp. isolated from chromate contaminated sediment |
| title_fullStr | Metabolic and genomic analysis elucidates strain-level variation in Microbacterium spp. isolated from chromate contaminated sediment |
| title_full_unstemmed | Metabolic and genomic analysis elucidates strain-level variation in Microbacterium spp. isolated from chromate contaminated sediment |
| title_short | Metabolic and genomic analysis elucidates strain-level variation in Microbacterium spp. isolated from chromate contaminated sediment |
| title_sort | metabolic and genomic analysis elucidates strain level variation in microbacterium spp isolated from chromate contaminated sediment |
| topic | Microbacterium Chromium reduction Strain-level variation |
| url | https://peerj.com/articles/1395.pdf |
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