Collective analysis of the expression and prognosis for LEM-domain proteins in prostate cancer
Abstract Background Mammalian LEM-domain proteins (LEMs) are encoded by seven genes, including LAP2, EMD, LEMD1, LEMD2, LEMD3, ANKLE1, and ANKLE2. Though some LEMs were involved in various tumor progression, the expression and prognostic values of LEMs in prostate adenocarcinoma (PRAD) have yet to b...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-06-01
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| Series: | World Journal of Surgical Oncology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12957-022-02640-z |
| _version_ | 1817968925872226304 |
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| author | Tianzhen He Yulian Zhang Xueyu Li Caihong Liu Guanqun Zhu Xinbao Yin Zongliang Zhang Kai Zhao Zhenlin Wang Peng Zhao Ke Wang |
| author_facet | Tianzhen He Yulian Zhang Xueyu Li Caihong Liu Guanqun Zhu Xinbao Yin Zongliang Zhang Kai Zhao Zhenlin Wang Peng Zhao Ke Wang |
| author_sort | Tianzhen He |
| collection | DOAJ |
| description | Abstract Background Mammalian LEM-domain proteins (LEMs) are encoded by seven genes, including LAP2, EMD, LEMD1, LEMD2, LEMD3, ANKLE1, and ANKLE2. Though some LEMs were involved in various tumor progression, the expression and prognostic values of LEMs in prostate adenocarcinoma (PRAD) have yet to be analyzed. Methods Herein, we investigated the expression, survival data, and immune infiltration levels of LEMs in PRAD patients from ATCG, TIMER, LinkedOmics, and TISIDB databases. We also further validated the mRNA and protein expression levels of ANKLE1, EMD, and LEMD2 in human prostate tumor specimens by qPCR, WB, and IHC. Results We found that all LEM expressions, except for that of LAP2, were markedly altered in PRAD compared to the normal samples. Among all LEMs, only the expressions of ANKLE1, EMD, and LEMD2 were correlated with advanced tumor stage and survival prognosis in PRAD. Consistent with the predicted computational results, the mRNA and protein expression levels of these genes were markedly increased in the PRAD group. We then found that ANKLE1, EMD, and LEMD2 expressions were markedly correlated with immune cell infiltration levels. High ANKLE1, EMD, and LEMD2 expressions predicted a worse prognosis in PRAD based on immune cells. DNA methylation or/and copy number variations may contribute to the abnormal upregulation of ANKLE1, EMD, and LEMD2 in PRAD. Conclusions Taken together, this study implied that ANKLE1, EMD, and LEMD2 were promising prognosis predictors and potential immunotherapy targets for PRAD patients. |
| first_indexed | 2024-04-13T20:15:01Z |
| format | Article |
| id | doaj.art-de3851594f1f4b35b4d0bc149c4ce433 |
| institution | Directory Open Access Journal |
| issn | 1477-7819 |
| language | English |
| last_indexed | 2024-04-13T20:15:01Z |
| publishDate | 2022-06-01 |
| publisher | BMC |
| record_format | Article |
| series | World Journal of Surgical Oncology |
| spelling | doaj.art-de3851594f1f4b35b4d0bc149c4ce4332022-12-22T02:31:44ZengBMCWorld Journal of Surgical Oncology1477-78192022-06-0120111610.1186/s12957-022-02640-zCollective analysis of the expression and prognosis for LEM-domain proteins in prostate cancerTianzhen He0Yulian Zhang1Xueyu Li2Caihong Liu3Guanqun Zhu4Xinbao Yin5Zongliang Zhang6Kai Zhao7Zhenlin Wang8Peng Zhao9Ke Wang10Institute of Special Environmental Medicine, Nantong UniversityDepartment of Gynecology, The Affiliated Hospital of Qingdao University, Qingdao UniversityDepartment of Urology, The Affiliated Hospital of Qingdao University, Qingdao UniversityWestern Administrative Office Center, Qingdao West Coast New District Health BureauDepartment of Urology, The Affiliated Hospital of Qingdao University, Qingdao UniversityDepartment of Urology, The Affiliated Hospital of Qingdao University, Qingdao UniversityDepartment of Urology, The Affiliated Hospital of Qingdao University, Qingdao UniversityDepartment of Urology, The Affiliated Hospital of Qingdao University, Qingdao UniversityDepartment of Urology, The Affiliated Hospital of Qingdao University, Qingdao UniversityFaculty of Sport Science and Coaching, Universiti Pendidikan Sultan IdrisDepartment of Urology, The Affiliated Hospital of Qingdao University, Qingdao UniversityAbstract Background Mammalian LEM-domain proteins (LEMs) are encoded by seven genes, including LAP2, EMD, LEMD1, LEMD2, LEMD3, ANKLE1, and ANKLE2. Though some LEMs were involved in various tumor progression, the expression and prognostic values of LEMs in prostate adenocarcinoma (PRAD) have yet to be analyzed. Methods Herein, we investigated the expression, survival data, and immune infiltration levels of LEMs in PRAD patients from ATCG, TIMER, LinkedOmics, and TISIDB databases. We also further validated the mRNA and protein expression levels of ANKLE1, EMD, and LEMD2 in human prostate tumor specimens by qPCR, WB, and IHC. Results We found that all LEM expressions, except for that of LAP2, were markedly altered in PRAD compared to the normal samples. Among all LEMs, only the expressions of ANKLE1, EMD, and LEMD2 were correlated with advanced tumor stage and survival prognosis in PRAD. Consistent with the predicted computational results, the mRNA and protein expression levels of these genes were markedly increased in the PRAD group. We then found that ANKLE1, EMD, and LEMD2 expressions were markedly correlated with immune cell infiltration levels. High ANKLE1, EMD, and LEMD2 expressions predicted a worse prognosis in PRAD based on immune cells. DNA methylation or/and copy number variations may contribute to the abnormal upregulation of ANKLE1, EMD, and LEMD2 in PRAD. Conclusions Taken together, this study implied that ANKLE1, EMD, and LEMD2 were promising prognosis predictors and potential immunotherapy targets for PRAD patients.https://doi.org/10.1186/s12957-022-02640-zLEM-domain proteinsProstate adenocarcinomaImmune infiltrationSurvival prognosisGene mutations |
| spellingShingle | Tianzhen He Yulian Zhang Xueyu Li Caihong Liu Guanqun Zhu Xinbao Yin Zongliang Zhang Kai Zhao Zhenlin Wang Peng Zhao Ke Wang Collective analysis of the expression and prognosis for LEM-domain proteins in prostate cancer World Journal of Surgical Oncology LEM-domain proteins Prostate adenocarcinoma Immune infiltration Survival prognosis Gene mutations |
| title | Collective analysis of the expression and prognosis for LEM-domain proteins in prostate cancer |
| title_full | Collective analysis of the expression and prognosis for LEM-domain proteins in prostate cancer |
| title_fullStr | Collective analysis of the expression and prognosis for LEM-domain proteins in prostate cancer |
| title_full_unstemmed | Collective analysis of the expression and prognosis for LEM-domain proteins in prostate cancer |
| title_short | Collective analysis of the expression and prognosis for LEM-domain proteins in prostate cancer |
| title_sort | collective analysis of the expression and prognosis for lem domain proteins in prostate cancer |
| topic | LEM-domain proteins Prostate adenocarcinoma Immune infiltration Survival prognosis Gene mutations |
| url | https://doi.org/10.1186/s12957-022-02640-z |
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