Proteomic and metabolomic profiling of acupuncture for migraine reveals a correlative link via energy metabolism
Migraine is a neurovascular disease with a high disability rate. Acupuncture treatment has emerged as a safe and viable alternative prophylactic therapy that can effectively alleviate the duration and frequency of migraine attacks. However, the therapeutic mechanisms underlying the effects of acupun...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnins.2022.1013328/full |
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| author | Lu Liu Weizheng Li Weizheng Li Linpeng Wang Pengyun Gong Pengyun Gong Tianli Lyu Dapeng Liu Yajie Zhang Yijie Guo Yijie Guo Xiang Liu Xiang Liu Min Tang Min Tang Hongke Hu Hongke Hu Chao Liu Chao Liu Bin Li |
| author_facet | Lu Liu Weizheng Li Weizheng Li Linpeng Wang Pengyun Gong Pengyun Gong Tianli Lyu Dapeng Liu Yajie Zhang Yijie Guo Yijie Guo Xiang Liu Xiang Liu Min Tang Min Tang Hongke Hu Hongke Hu Chao Liu Chao Liu Bin Li |
| author_sort | Lu Liu |
| collection | DOAJ |
| description | Migraine is a neurovascular disease with a high disability rate. Acupuncture treatment has emerged as a safe and viable alternative prophylactic therapy that can effectively alleviate the duration and frequency of migraine attacks. However, the therapeutic mechanisms underlying the effects of acupuncture are yet to be systematically elucidated. In this study, we enrolled female patients with migraine without aura (n = 20) and healthy controls (n = 10). Patients received acupuncture treatment on DU20, DU24, bilateral GB13, GB8, and GB20, applied three times per week over the course of 4 weeks for 12 sessions in total. Blood samples were collected from the median cubital vein before and after acupuncture treatment. Proteomic and metabolomic profiling was performed using liquid chromatography-mass spectrometry to determine the characteristics of differentially expressed molecules and expression of their corresponding biological pathways as well as to elucidate the pathogenesis of migraine and the biological effects underlying the treatment of migraine with acupuncture. Proteomic and metabolomic profiling of plasma samples from patients with migraine without aura before and after acupuncture treatment revealed enrichment of immune-related pathway functions and the arginine synthesis pathway. Joint pathway analyses revealed significant enrichment of the pentose phosphate and glycolysis/gluconeogenesis pathways in patients with migraine. The glycolysis/gluconeogenesis and riboflavin metabolism pathways were significantly enriched after acupuncture treatment. The expression levels of various key proteins and metabolites, including α-D-glucose, flavin adenine dinucleotide, biliverdin reductase B, and L-glutamate, were significantly differentially expressed before and after acupuncture treatment in patients with migraine without aura. Treatment of migraine with acupuncture was associated with significant changes in key molecules and pathways, indicative of physiological changes in the trigeminovascular system, glutamate neurotoxicity, and other migraine-related physiological changes. Overall, our comprehensive analysis using proteomic and metabolomic profiling demonstrates that energy metabolism may serve as a key correlative link in the occurrence of migraine and the therapeutic effects of acupuncture treatment. Our findings may facilitate the identification of diagnostic and therapeutic modalities in the ongoing search for effective treatments for migraine attacks. |
| first_indexed | 2024-04-12T04:18:21Z |
| format | Article |
| id | doaj.art-de3b604c1685474e8aaf584e81c7d42b |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-04-12T04:18:21Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-de3b604c1685474e8aaf584e81c7d42b2022-12-22T03:48:20ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2022-09-011610.3389/fnins.2022.10133281013328Proteomic and metabolomic profiling of acupuncture for migraine reveals a correlative link via energy metabolismLu Liu0Weizheng Li1Weizheng Li2Linpeng Wang3Pengyun Gong4Pengyun Gong5Tianli Lyu6Dapeng Liu7Yajie Zhang8Yijie Guo9Yijie Guo10Xiang Liu11Xiang Liu12Min Tang13Min Tang14Hongke Hu15Hongke Hu16Chao Liu17Chao Liu18Bin Li19Beijing Key Laboratory of Acupuncture Neuromodulation, Department of Acupuncture and Moxibustion, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing, ChinaSchool of Engineering Medicine & School of Biological Science and Medical Engineering, Beihang University, Beijing, ChinaKey Laboratory of Big Data-Based Precision Medicine, Beihang University, Ministry of Industry and Information Technology, Beijing, ChinaBeijing Key Laboratory of Acupuncture Neuromodulation, Department of Acupuncture and Moxibustion, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing, ChinaSchool of Engineering Medicine & School of Biological Science and Medical Engineering, Beihang University, Beijing, ChinaKey Laboratory of Big Data-Based Precision Medicine, Beihang University, Ministry of Industry and Information Technology, Beijing, ChinaBeijing Key Laboratory of Acupuncture Neuromodulation, Department of Acupuncture and Moxibustion, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing, ChinaThird Affiliated Hospital, Beijing University of Chinese Medicine, Beijing, ChinaShanxi Hospital of Integrated Traditional and Western Medicine, Taiyuan, ChinaSchool of Engineering Medicine & School of Biological Science and Medical Engineering, Beihang University, Beijing, ChinaKey Laboratory of Big Data-Based Precision Medicine, Beihang University, Ministry of Industry and Information Technology, Beijing, ChinaSchool of Engineering Medicine & School of Biological Science and Medical Engineering, Beihang University, Beijing, ChinaKey Laboratory of Big Data-Based Precision Medicine, Beihang University, Ministry of Industry and Information Technology, Beijing, ChinaSchool of Engineering Medicine & School of Biological Science and Medical Engineering, Beihang University, Beijing, ChinaKey Laboratory of Big Data-Based Precision Medicine, Beihang University, Ministry of Industry and Information Technology, Beijing, ChinaSchool of Engineering Medicine & School of Biological Science and Medical Engineering, Beihang University, Beijing, ChinaKey Laboratory of Big Data-Based Precision Medicine, Beihang University, Ministry of Industry and Information Technology, Beijing, ChinaSchool of Engineering Medicine & School of Biological Science and Medical Engineering, Beihang University, Beijing, ChinaKey Laboratory of Big Data-Based Precision Medicine, Beihang University, Ministry of Industry and Information Technology, Beijing, ChinaBeijing Key Laboratory of Acupuncture Neuromodulation, Department of Acupuncture and Moxibustion, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing, ChinaMigraine is a neurovascular disease with a high disability rate. Acupuncture treatment has emerged as a safe and viable alternative prophylactic therapy that can effectively alleviate the duration and frequency of migraine attacks. However, the therapeutic mechanisms underlying the effects of acupuncture are yet to be systematically elucidated. In this study, we enrolled female patients with migraine without aura (n = 20) and healthy controls (n = 10). Patients received acupuncture treatment on DU20, DU24, bilateral GB13, GB8, and GB20, applied three times per week over the course of 4 weeks for 12 sessions in total. Blood samples were collected from the median cubital vein before and after acupuncture treatment. Proteomic and metabolomic profiling was performed using liquid chromatography-mass spectrometry to determine the characteristics of differentially expressed molecules and expression of their corresponding biological pathways as well as to elucidate the pathogenesis of migraine and the biological effects underlying the treatment of migraine with acupuncture. Proteomic and metabolomic profiling of plasma samples from patients with migraine without aura before and after acupuncture treatment revealed enrichment of immune-related pathway functions and the arginine synthesis pathway. Joint pathway analyses revealed significant enrichment of the pentose phosphate and glycolysis/gluconeogenesis pathways in patients with migraine. The glycolysis/gluconeogenesis and riboflavin metabolism pathways were significantly enriched after acupuncture treatment. The expression levels of various key proteins and metabolites, including α-D-glucose, flavin adenine dinucleotide, biliverdin reductase B, and L-glutamate, were significantly differentially expressed before and after acupuncture treatment in patients with migraine without aura. Treatment of migraine with acupuncture was associated with significant changes in key molecules and pathways, indicative of physiological changes in the trigeminovascular system, glutamate neurotoxicity, and other migraine-related physiological changes. Overall, our comprehensive analysis using proteomic and metabolomic profiling demonstrates that energy metabolism may serve as a key correlative link in the occurrence of migraine and the therapeutic effects of acupuncture treatment. Our findings may facilitate the identification of diagnostic and therapeutic modalities in the ongoing search for effective treatments for migraine attacks.https://www.frontiersin.org/articles/10.3389/fnins.2022.1013328/fullmigraineproteomicsmetabolomicsacupunctureenergy metabolism pathways |
| spellingShingle | Lu Liu Weizheng Li Weizheng Li Linpeng Wang Pengyun Gong Pengyun Gong Tianli Lyu Dapeng Liu Yajie Zhang Yijie Guo Yijie Guo Xiang Liu Xiang Liu Min Tang Min Tang Hongke Hu Hongke Hu Chao Liu Chao Liu Bin Li Proteomic and metabolomic profiling of acupuncture for migraine reveals a correlative link via energy metabolism Frontiers in Neuroscience migraine proteomics metabolomics acupuncture energy metabolism pathways |
| title | Proteomic and metabolomic profiling of acupuncture for migraine reveals a correlative link via energy metabolism |
| title_full | Proteomic and metabolomic profiling of acupuncture for migraine reveals a correlative link via energy metabolism |
| title_fullStr | Proteomic and metabolomic profiling of acupuncture for migraine reveals a correlative link via energy metabolism |
| title_full_unstemmed | Proteomic and metabolomic profiling of acupuncture for migraine reveals a correlative link via energy metabolism |
| title_short | Proteomic and metabolomic profiling of acupuncture for migraine reveals a correlative link via energy metabolism |
| title_sort | proteomic and metabolomic profiling of acupuncture for migraine reveals a correlative link via energy metabolism |
| topic | migraine proteomics metabolomics acupuncture energy metabolism pathways |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2022.1013328/full |
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