Behavioral and Biochemical Evaluation of Anti-Depressive and Oxidative Stress-Ameliorating Effects of Amber Extract in Adult Male ICR Mice
Amber, a plant resin, exhibits an anti-stress effect and is used in traditional medicine. Recently, it has been speculated that amber may possess an anti-depressive effect. However, there is no evidence to support this efficacy. Thus, this study investigated the anti-depressive and oxidative-stress-...
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MDPI AG
2023-04-01
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| Series: | Nutraceuticals |
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| Online Access: | https://www.mdpi.com/1661-3821/3/2/17 |
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| author | Sandra Somuah-Asante Mahmoud Ben Othman Reiko Takeda Kazuma Okazaki Marie Sekita Kazuichi Sakamoto |
| author_facet | Sandra Somuah-Asante Mahmoud Ben Othman Reiko Takeda Kazuma Okazaki Marie Sekita Kazuichi Sakamoto |
| author_sort | Sandra Somuah-Asante |
| collection | DOAJ |
| description | Amber, a plant resin, exhibits an anti-stress effect and is used in traditional medicine. Recently, it has been speculated that amber may possess an anti-depressive effect. However, there is no evidence to support this efficacy. Thus, this study investigated the anti-depressive and oxidative-stress-ameliorating effects of amber extract in mice subjected to restraint stress. Mice were treated with amber extract (25 and 50 mg/kg, p.o.) and bupropion (10 mg/kg, p.o.) as positive control. Mice were then subjected to a tail suspension test, and their immobility time, body weight before and after stress, levels of stress-related hormones and neurotransmitters, and oxidative stress parameters were assessed. Amber supplementation did not affect the body weight of mice in any of the groups. Amber extract (25 and 50 mg/kg) demonstrated an anti-depressive effect by significantly decreasing the immobility time and adrenocorticotropin-hormone and corticosterone-hormone levels. Moreover, amber extract at a dose of 25 mg/kg increased the levels of dopamine and serotonin. Additionally, superoxide dismutase, catalase, and glutathione levels increased, whereas the malondialdehyde content decreased with amber supplementation. These findings confirm that amber may possess an anti-depressive effect and hence can be a useful alternative therapy for preventing and managing depression. |
| first_indexed | 2024-03-11T02:05:44Z |
| format | Article |
| id | doaj.art-de3ce1ef48cf42ee8eb77b57c98c6fde |
| institution | Directory Open Access Journal |
| issn | 1661-3821 |
| language | English |
| last_indexed | 2024-03-11T02:05:44Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutraceuticals |
| spelling | doaj.art-de3ce1ef48cf42ee8eb77b57c98c6fde2023-11-18T11:55:25ZengMDPI AGNutraceuticals1661-38212023-04-013222223010.3390/nutraceuticals3020017Behavioral and Biochemical Evaluation of Anti-Depressive and Oxidative Stress-Ameliorating Effects of Amber Extract in Adult Male ICR MiceSandra Somuah-Asante0Mahmoud Ben Othman1Reiko Takeda2Kazuma Okazaki3Marie Sekita4Kazuichi Sakamoto5Tsukuba Life Science Innovation Program (T-LSI), University of Tsukuba, Tennodai 1-1-1, Tsukuba 305-8577, JapanFaculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, JapanFaculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, JapanKohaku Bio Technology Co., Ltd., Tsukuba 305-8572, JapanKohaku Bio Technology Co., Ltd., Tsukuba 305-8572, JapanTsukuba Life Science Innovation Program (T-LSI), University of Tsukuba, Tennodai 1-1-1, Tsukuba 305-8577, JapanAmber, a plant resin, exhibits an anti-stress effect and is used in traditional medicine. Recently, it has been speculated that amber may possess an anti-depressive effect. However, there is no evidence to support this efficacy. Thus, this study investigated the anti-depressive and oxidative-stress-ameliorating effects of amber extract in mice subjected to restraint stress. Mice were treated with amber extract (25 and 50 mg/kg, p.o.) and bupropion (10 mg/kg, p.o.) as positive control. Mice were then subjected to a tail suspension test, and their immobility time, body weight before and after stress, levels of stress-related hormones and neurotransmitters, and oxidative stress parameters were assessed. Amber supplementation did not affect the body weight of mice in any of the groups. Amber extract (25 and 50 mg/kg) demonstrated an anti-depressive effect by significantly decreasing the immobility time and adrenocorticotropin-hormone and corticosterone-hormone levels. Moreover, amber extract at a dose of 25 mg/kg increased the levels of dopamine and serotonin. Additionally, superoxide dismutase, catalase, and glutathione levels increased, whereas the malondialdehyde content decreased with amber supplementation. These findings confirm that amber may possess an anti-depressive effect and hence can be a useful alternative therapy for preventing and managing depression.https://www.mdpi.com/1661-3821/3/2/17amberantidepressantHPA axisoxidative stressstress hormones |
| spellingShingle | Sandra Somuah-Asante Mahmoud Ben Othman Reiko Takeda Kazuma Okazaki Marie Sekita Kazuichi Sakamoto Behavioral and Biochemical Evaluation of Anti-Depressive and Oxidative Stress-Ameliorating Effects of Amber Extract in Adult Male ICR Mice Nutraceuticals amber antidepressant HPA axis oxidative stress stress hormones |
| title | Behavioral and Biochemical Evaluation of Anti-Depressive and Oxidative Stress-Ameliorating Effects of Amber Extract in Adult Male ICR Mice |
| title_full | Behavioral and Biochemical Evaluation of Anti-Depressive and Oxidative Stress-Ameliorating Effects of Amber Extract in Adult Male ICR Mice |
| title_fullStr | Behavioral and Biochemical Evaluation of Anti-Depressive and Oxidative Stress-Ameliorating Effects of Amber Extract in Adult Male ICR Mice |
| title_full_unstemmed | Behavioral and Biochemical Evaluation of Anti-Depressive and Oxidative Stress-Ameliorating Effects of Amber Extract in Adult Male ICR Mice |
| title_short | Behavioral and Biochemical Evaluation of Anti-Depressive and Oxidative Stress-Ameliorating Effects of Amber Extract in Adult Male ICR Mice |
| title_sort | behavioral and biochemical evaluation of anti depressive and oxidative stress ameliorating effects of amber extract in adult male icr mice |
| topic | amber antidepressant HPA axis oxidative stress stress hormones |
| url | https://www.mdpi.com/1661-3821/3/2/17 |
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