Detection of 17β-Estradiol in Foods by a Fluorescence Aptasensor Based on Gold Nanoparticles
A fluorescent aptasensor was constructed for the high-sensitivity and high-selectivity detection of 17β-estradiol (E2) based on the fluorescence quenching effect of gold nanoparticles (AuNPs) on carbon quantum dots (CQDs) and the specific affinity of aptamers to targets. Under the optimal conditions...
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| Format: | Article |
| Language: | English |
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China Food Publishing Company
2023-07-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-14-043.pdf |
| _version_ | 1797742559947128832 |
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| author | WEI Qingyi, LIN Xuanran, ZHANG Peiyao, SUN Da-wen, PU Hongbin |
| author_facet | WEI Qingyi, LIN Xuanran, ZHANG Peiyao, SUN Da-wen, PU Hongbin |
| author_sort | WEI Qingyi, LIN Xuanran, ZHANG Peiyao, SUN Da-wen, PU Hongbin |
| collection | DOAJ |
| description | A fluorescent aptasensor was constructed for the high-sensitivity and high-selectivity detection of 17β-estradiol (E2) based on the fluorescence quenching effect of gold nanoparticles (AuNPs) on carbon quantum dots (CQDs) and the specific affinity of aptamers to targets. Under the optimal conditions (particle size of AuNPs = 39 nm, AuNPs amount = 200 μL, quenching time = 20 min, and fluorescence recovery time = 60 min), a good linear relationship was observed between the fluorescence recovery degree (y) of the sensor and the logarithmic value (x) of E2 concentration (2 × 10-5–2 × 10-9 mol/L), which was fitted with the following equation: y = 58.50x + 558.95 with a determination coefficient (R2) of 0.992. The detection of limit (LOD) of E2 was 3.4 × 10-10 mol/L. Finally, the aptasensor was successfully applied to the detection of E2 spiked in milk and environmental water samples with good recoveries of 89.67%‒113.36%, suggesting the potential for practical application. |
| first_indexed | 2024-03-12T14:42:26Z |
| format | Article |
| id | doaj.art-de3f5ce043b14c48a1b6fcac595aa0c9 |
| institution | Directory Open Access Journal |
| issn | 1002-6630 |
| language | English |
| last_indexed | 2024-03-12T14:42:26Z |
| publishDate | 2023-07-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj.art-de3f5ce043b14c48a1b6fcac595aa0c92023-08-16T05:39:29ZengChina Food Publishing CompanyShipin Kexue1002-66302023-07-01441436837610.7506/spkx1002-6630-20220630-340Detection of 17β-Estradiol in Foods by a Fluorescence Aptasensor Based on Gold NanoparticlesWEI Qingyi, LIN Xuanran, ZHANG Peiyao, SUN Da-wen, PU Hongbin0(Academy of Contemporary Food Engineering, Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods, School of Food Science and Engineering, South China University of Technology, Guangzhou 510006, China)A fluorescent aptasensor was constructed for the high-sensitivity and high-selectivity detection of 17β-estradiol (E2) based on the fluorescence quenching effect of gold nanoparticles (AuNPs) on carbon quantum dots (CQDs) and the specific affinity of aptamers to targets. Under the optimal conditions (particle size of AuNPs = 39 nm, AuNPs amount = 200 μL, quenching time = 20 min, and fluorescence recovery time = 60 min), a good linear relationship was observed between the fluorescence recovery degree (y) of the sensor and the logarithmic value (x) of E2 concentration (2 × 10-5–2 × 10-9 mol/L), which was fitted with the following equation: y = 58.50x + 558.95 with a determination coefficient (R2) of 0.992. The detection of limit (LOD) of E2 was 3.4 × 10-10 mol/L. Finally, the aptasensor was successfully applied to the detection of E2 spiked in milk and environmental water samples with good recoveries of 89.67%‒113.36%, suggesting the potential for practical application.https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-14-043.pdfcarbon quantum dots; gold nanoparticles; 17β-estradiol; aptasensor; milk |
| spellingShingle | WEI Qingyi, LIN Xuanran, ZHANG Peiyao, SUN Da-wen, PU Hongbin Detection of 17β-Estradiol in Foods by a Fluorescence Aptasensor Based on Gold Nanoparticles Shipin Kexue carbon quantum dots; gold nanoparticles; 17β-estradiol; aptasensor; milk |
| title | Detection of 17β-Estradiol in Foods by a Fluorescence Aptasensor Based on Gold Nanoparticles |
| title_full | Detection of 17β-Estradiol in Foods by a Fluorescence Aptasensor Based on Gold Nanoparticles |
| title_fullStr | Detection of 17β-Estradiol in Foods by a Fluorescence Aptasensor Based on Gold Nanoparticles |
| title_full_unstemmed | Detection of 17β-Estradiol in Foods by a Fluorescence Aptasensor Based on Gold Nanoparticles |
| title_short | Detection of 17β-Estradiol in Foods by a Fluorescence Aptasensor Based on Gold Nanoparticles |
| title_sort | detection of 17β estradiol in foods by a fluorescence aptasensor based on gold nanoparticles |
| topic | carbon quantum dots; gold nanoparticles; 17β-estradiol; aptasensor; milk |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-14-043.pdf |
| work_keys_str_mv | AT weiqingyilinxuanranzhangpeiyaosundawenpuhongbin detectionof17bestradiolinfoodsbyafluorescenceaptasensorbasedongoldnanoparticles |