Mutation of the rice XA21 predicted nuclear localization sequence does not affect resistance to Xanthomonas oryzae pv. oryzae
Background The rice receptor kinase XA21 confers robust resistance to the bacterial pathogen Xanthomonas oryzaepv. oryzae(Xoo). We previously reported that XA21 is cleaved in transgenic plants overexpressing XA21 with a GFP tag (Ubi-XA21-GFP) and that the released C-terminal domain is localized to t...
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PeerJ Inc.
2016-10-01
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| Online Access: | https://peerj.com/articles/2507.pdf |
| _version_ | 1797419115124621312 |
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| author | Tong Wei Tsung-Chi Chen Yuen Ting Ho Pamela C. Ronald |
| author_facet | Tong Wei Tsung-Chi Chen Yuen Ting Ho Pamela C. Ronald |
| author_sort | Tong Wei |
| collection | DOAJ |
| description | Background The rice receptor kinase XA21 confers robust resistance to the bacterial pathogen Xanthomonas oryzaepv. oryzae(Xoo). We previously reported that XA21 is cleaved in transgenic plants overexpressing XA21 with a GFP tag (Ubi-XA21-GFP) and that the released C-terminal domain is localized to the nucleus. XA21 carries a predicted nuclear localization sequence (NLS) that directs the C-terminal domain to the nucleus in transient assays, whereas alanine substitutions in the NLS disrupt the nuclear localization. Methods To determine if the predicted NLS is required for XA21-mediated immunity in planta, we generated transgenic plants overexpressing an XA21 variant carrying the NLS with the same alanine substitutions (Ubi-XA21nls-GFP). Results Ubi-XA21nls-GFP plants displayed slightly longer lesion lengths, higher Xoobacterial populations after inoculation and lower levels of reactive oxygen species production compared with the Ubi-XA21-GFP control plants. However, the Ubi-XA21nls-GFP plants express lower levels of protein than that observed in Ubi-XA21-GFP. Discussion These results demonstrate that the predicted NLS is not required for XA21-mediated immunity. |
| first_indexed | 2024-03-09T06:43:48Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:43:48Z |
| publishDate | 2016-10-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-de407f990a544fe19a96642a1867a4062023-12-03T10:43:14ZengPeerJ Inc.PeerJ2167-83592016-10-014e250710.7717/peerj.2507Mutation of the rice XA21 predicted nuclear localization sequence does not affect resistance to Xanthomonas oryzae pv. oryzaeTong Wei0Tsung-Chi Chen1Yuen Ting Ho2Pamela C. Ronald3Department of Plant Pathology and the Genome Center, University of California, Davis, CA, United StatesDepartment of Plant Pathology and the Genome Center, University of California, Davis, CA, United StatesDepartment of Plant Pathology and the Genome Center, University of California, Davis, CA, United StatesDepartment of Plant Pathology and the Genome Center, University of California, Davis, CA, United StatesBackground The rice receptor kinase XA21 confers robust resistance to the bacterial pathogen Xanthomonas oryzaepv. oryzae(Xoo). We previously reported that XA21 is cleaved in transgenic plants overexpressing XA21 with a GFP tag (Ubi-XA21-GFP) and that the released C-terminal domain is localized to the nucleus. XA21 carries a predicted nuclear localization sequence (NLS) that directs the C-terminal domain to the nucleus in transient assays, whereas alanine substitutions in the NLS disrupt the nuclear localization. Methods To determine if the predicted NLS is required for XA21-mediated immunity in planta, we generated transgenic plants overexpressing an XA21 variant carrying the NLS with the same alanine substitutions (Ubi-XA21nls-GFP). Results Ubi-XA21nls-GFP plants displayed slightly longer lesion lengths, higher Xoobacterial populations after inoculation and lower levels of reactive oxygen species production compared with the Ubi-XA21-GFP control plants. However, the Ubi-XA21nls-GFP plants express lower levels of protein than that observed in Ubi-XA21-GFP. Discussion These results demonstrate that the predicted NLS is not required for XA21-mediated immunity.https://peerj.com/articles/2507.pdfNuclear localizationBacterial blight diseaseXanthomonas oryzae pv. oryzaeXA21-mediated immunity |
| spellingShingle | Tong Wei Tsung-Chi Chen Yuen Ting Ho Pamela C. Ronald Mutation of the rice XA21 predicted nuclear localization sequence does not affect resistance to Xanthomonas oryzae pv. oryzae PeerJ Nuclear localization Bacterial blight disease Xanthomonas oryzae pv. oryzae XA21-mediated immunity |
| title | Mutation of the rice XA21 predicted nuclear localization sequence does not affect resistance to Xanthomonas oryzae pv. oryzae |
| title_full | Mutation of the rice XA21 predicted nuclear localization sequence does not affect resistance to Xanthomonas oryzae pv. oryzae |
| title_fullStr | Mutation of the rice XA21 predicted nuclear localization sequence does not affect resistance to Xanthomonas oryzae pv. oryzae |
| title_full_unstemmed | Mutation of the rice XA21 predicted nuclear localization sequence does not affect resistance to Xanthomonas oryzae pv. oryzae |
| title_short | Mutation of the rice XA21 predicted nuclear localization sequence does not affect resistance to Xanthomonas oryzae pv. oryzae |
| title_sort | mutation of the rice xa21 predicted nuclear localization sequence does not affect resistance to xanthomonas oryzae pv oryzae |
| topic | Nuclear localization Bacterial blight disease Xanthomonas oryzae pv. oryzae XA21-mediated immunity |
| url | https://peerj.com/articles/2507.pdf |
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