Isobavachalcone inhibits Pseudorabies virus by impairing virus-induced cell-to-cell fusion
Abstract Pseudorabies virus (PRV) is an important pathogen that threatens the global swine industry. Currently, there is no effective drug that can clinically prevent or treat PRV infections. Isobavachalcone (IBC), a natural chalcone compound derived from Psoralea corylifolia, displays multiple biol...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-03-01
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| Series: | Virology Journal |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12985-020-01312-2 |
| _version_ | 1818447480679825408 |
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| author | Yu Wang Tian-Xin Liu Tong-Yun Wang Yan-Dong Tang Ping Wei |
| author_facet | Yu Wang Tian-Xin Liu Tong-Yun Wang Yan-Dong Tang Ping Wei |
| author_sort | Yu Wang |
| collection | DOAJ |
| description | Abstract Pseudorabies virus (PRV) is an important pathogen that threatens the global swine industry. Currently, there is no effective drug that can clinically prevent or treat PRV infections. Isobavachalcone (IBC), a natural chalcone compound derived from Psoralea corylifolia, displays multiple biological activities, such as antibacterial, antifungal, and anticancer activities. Recently, it was found that IBC exhibited antiviral activity against an RNA virus, porcine reproductive and respiratory syndrome virus (PRRSV), in vitro. In the current study, we further demonstrated for the first time that IBC has a strong inhibitory effect on PRV. Through a viral luciferase expression assay, we showed that the inhibition step occurs mainly in the late stage of viral replication. Finally, via a cell-to-cell fusion assay, we demonstrated that IBC inhibits PRV by blocking virus-mediated cell fusion. Thus, IBC may be a candidate for further therapeutic evaluation against PRV infection in vivo. |
| first_indexed | 2024-12-14T20:04:18Z |
| format | Article |
| id | doaj.art-de42e02989cd4b5885f5c89ecdf9815b |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-14T20:04:18Z |
| publishDate | 2020-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-de42e02989cd4b5885f5c89ecdf9815b2022-12-21T22:49:05ZengBMCVirology Journal1743-422X2020-03-011711410.1186/s12985-020-01312-2Isobavachalcone inhibits Pseudorabies virus by impairing virus-induced cell-to-cell fusionYu Wang0Tian-Xin Liu1Tong-Yun Wang2Yan-Dong Tang3Ping Wei4College of Veterinary Medicine, Northeast Agricultural UniversityState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural SciencesState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural SciencesState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural SciencesCollege of Veterinary Medicine, Northeast Agricultural UniversityAbstract Pseudorabies virus (PRV) is an important pathogen that threatens the global swine industry. Currently, there is no effective drug that can clinically prevent or treat PRV infections. Isobavachalcone (IBC), a natural chalcone compound derived from Psoralea corylifolia, displays multiple biological activities, such as antibacterial, antifungal, and anticancer activities. Recently, it was found that IBC exhibited antiviral activity against an RNA virus, porcine reproductive and respiratory syndrome virus (PRRSV), in vitro. In the current study, we further demonstrated for the first time that IBC has a strong inhibitory effect on PRV. Through a viral luciferase expression assay, we showed that the inhibition step occurs mainly in the late stage of viral replication. Finally, via a cell-to-cell fusion assay, we demonstrated that IBC inhibits PRV by blocking virus-mediated cell fusion. Thus, IBC may be a candidate for further therapeutic evaluation against PRV infection in vivo.http://link.springer.com/article/10.1186/s12985-020-01312-2PRVIsobavachalconeAntiviralFusion |
| spellingShingle | Yu Wang Tian-Xin Liu Tong-Yun Wang Yan-Dong Tang Ping Wei Isobavachalcone inhibits Pseudorabies virus by impairing virus-induced cell-to-cell fusion Virology Journal PRV Isobavachalcone Antiviral Fusion |
| title | Isobavachalcone inhibits Pseudorabies virus by impairing virus-induced cell-to-cell fusion |
| title_full | Isobavachalcone inhibits Pseudorabies virus by impairing virus-induced cell-to-cell fusion |
| title_fullStr | Isobavachalcone inhibits Pseudorabies virus by impairing virus-induced cell-to-cell fusion |
| title_full_unstemmed | Isobavachalcone inhibits Pseudorabies virus by impairing virus-induced cell-to-cell fusion |
| title_short | Isobavachalcone inhibits Pseudorabies virus by impairing virus-induced cell-to-cell fusion |
| title_sort | isobavachalcone inhibits pseudorabies virus by impairing virus induced cell to cell fusion |
| topic | PRV Isobavachalcone Antiviral Fusion |
| url | http://link.springer.com/article/10.1186/s12985-020-01312-2 |
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