Chemical compounds of extracts from Sarcodon imbricatus at optimized growth conditions
The effect of carbon and nitrogen sources and initial pH and temperature of the medium on the mycelial growth of Sarcodon imbricatus (L.) P. Karst. in axenic liquid culture was investigated. The optimal composition of the medium was found to be: 5% fructose, 1% hydrolysate of casein, 1% yeast extrac...
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| Format: | Article |
| Language: | English |
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Polish Botanical Society
2016-12-01
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| Series: | Acta Mycologica |
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| Online Access: | https://pbsociety.org.pl/journals/index.php/am/article/view/6666 |
| _version_ | 1828409187631104000 |
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| author | Katarzyna Sułkowska-Ziaja Agnieszka Szewczyk Joanna Gdula-Argasińska Halina Ekiert Jerzy Jaśkiewicz Bożena Muszyńska |
| author_facet | Katarzyna Sułkowska-Ziaja Agnieszka Szewczyk Joanna Gdula-Argasińska Halina Ekiert Jerzy Jaśkiewicz Bożena Muszyńska |
| author_sort | Katarzyna Sułkowska-Ziaja |
| collection | DOAJ |
| description | The effect of carbon and nitrogen sources and initial pH and temperature of the medium on the mycelial growth of Sarcodon imbricatus (L.) P. Karst. in axenic liquid culture was investigated. The optimal composition of the medium was found to be: 5% fructose, 1% hydrolysate of casein, 1% yeast extract, and 0.3% KH2PO4 at pH = 6 and incubation temperature of 20°C. In this condition the maximum biomass growth was observed, yielding 10.2 g L−1 of dry weight after 3-week of growth. The medium regarded as optimal for growth of S. imbricatus mycelium was used for the production of the biomass and further chemical analysis. The quantitative and qualitative composition of phenolic acids, fatty acids, and sterols were determined using chromatographic methods. The total content of phenolic acids was 1.86 mg × 100 g−1 DW, with the largest amount of protocatechuic acid (1.27 mg × 100 g−1 DW). Nineteen fatty acids were estimated, including five unsaturated fatty acids, e.g., oleic and α-linolenic acid. The analysis of sterols composition revealed the presence of ergosterol and ergosterol peroxide (197.7 and 200.47 mg × 100 g−1 DW, respectively). These compounds were isolated and confirmed by 1H-NMR. Presented study constitutes the first report on the accumulation of substances (phenolic acids, fatty acids, and sterols) with multidirectional biological activity in the mycelial axenic culture of Sarcodon imbricatus. |
| first_indexed | 2024-12-10T11:51:08Z |
| format | Article |
| id | doaj.art-de44af98fb7449c6b54ac2c2adbe8335 |
| institution | Directory Open Access Journal |
| issn | 2353-074X |
| language | English |
| last_indexed | 2024-12-10T11:51:08Z |
| publishDate | 2016-12-01 |
| publisher | Polish Botanical Society |
| record_format | Article |
| series | Acta Mycologica |
| spelling | doaj.art-de44af98fb7449c6b54ac2c2adbe83352022-12-22T01:49:56ZengPolish Botanical SocietyActa Mycologica2353-074X2016-12-0151210.5586/am.10865771Chemical compounds of extracts from Sarcodon imbricatus at optimized growth conditionsKatarzyna Sułkowska-Ziaja0Agnieszka Szewczyk1Joanna Gdula-Argasińska2Halina Ekiert3Jerzy Jaśkiewicz4Bożena Muszyńska5Department of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9, 30-688 KrakówDepartment of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9, 30-688 KrakówDepartment of Radioligands, Faculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9, 30-688 KrakówDepartment of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9, 30-688 KrakówFaculty of Health and Medical Science, Andrzej Frycz Modrzewski Kraków University, Gustawa Herlinga-Grudzińskiego 1, 30-705 KrakówDepartment of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9, 30-688 KrakówThe effect of carbon and nitrogen sources and initial pH and temperature of the medium on the mycelial growth of Sarcodon imbricatus (L.) P. Karst. in axenic liquid culture was investigated. The optimal composition of the medium was found to be: 5% fructose, 1% hydrolysate of casein, 1% yeast extract, and 0.3% KH2PO4 at pH = 6 and incubation temperature of 20°C. In this condition the maximum biomass growth was observed, yielding 10.2 g L−1 of dry weight after 3-week of growth. The medium regarded as optimal for growth of S. imbricatus mycelium was used for the production of the biomass and further chemical analysis. The quantitative and qualitative composition of phenolic acids, fatty acids, and sterols were determined using chromatographic methods. The total content of phenolic acids was 1.86 mg × 100 g−1 DW, with the largest amount of protocatechuic acid (1.27 mg × 100 g−1 DW). Nineteen fatty acids were estimated, including five unsaturated fatty acids, e.g., oleic and α-linolenic acid. The analysis of sterols composition revealed the presence of ergosterol and ergosterol peroxide (197.7 and 200.47 mg × 100 g−1 DW, respectively). These compounds were isolated and confirmed by 1H-NMR. Presented study constitutes the first report on the accumulation of substances (phenolic acids, fatty acids, and sterols) with multidirectional biological activity in the mycelial axenic culture of Sarcodon imbricatus.https://pbsociety.org.pl/journals/index.php/am/article/view/6666Sarcodon imbricatusin vitro culturessecondary metabolites |
| spellingShingle | Katarzyna Sułkowska-Ziaja Agnieszka Szewczyk Joanna Gdula-Argasińska Halina Ekiert Jerzy Jaśkiewicz Bożena Muszyńska Chemical compounds of extracts from Sarcodon imbricatus at optimized growth conditions Acta Mycologica Sarcodon imbricatus in vitro cultures secondary metabolites |
| title | Chemical compounds of extracts from Sarcodon imbricatus at optimized growth conditions |
| title_full | Chemical compounds of extracts from Sarcodon imbricatus at optimized growth conditions |
| title_fullStr | Chemical compounds of extracts from Sarcodon imbricatus at optimized growth conditions |
| title_full_unstemmed | Chemical compounds of extracts from Sarcodon imbricatus at optimized growth conditions |
| title_short | Chemical compounds of extracts from Sarcodon imbricatus at optimized growth conditions |
| title_sort | chemical compounds of extracts from sarcodon imbricatus at optimized growth conditions |
| topic | Sarcodon imbricatus in vitro cultures secondary metabolites |
| url | https://pbsociety.org.pl/journals/index.php/am/article/view/6666 |
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