Laboratory identification and clinical characteristics of Streptococcus bovis/Streptococcus equinus complex bacteremia: a retrospective, multicenter study in Hiroshima, Japan
Abstract Background Bacteremia due to the Streptococcus bovis/Streptococcus equinus complex (SBSEC) is associated with specific diseases, such as colorectal cancer and infective endocarditis. This study aimed to evaluate the clinical characteristics of SBSEC bacteremia and the accuracy of identifica...
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| Format: | Article |
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BMC
2021-11-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | https://doi.org/10.1186/s12879-021-06880-4 |
| _version_ | 1818433907000868864 |
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| author | Yuki Kaiki Hiroki Kitagawa Kayoko Tadera Hiroyuki Taogoshi Mitsuyasu Ikeda Mikihiro Kano Toshie Harino Toshihito Nomura Keitaro Omori Norifumi Shigemoto Shinya Takahashi Hiroki Ohge |
| author_facet | Yuki Kaiki Hiroki Kitagawa Kayoko Tadera Hiroyuki Taogoshi Mitsuyasu Ikeda Mikihiro Kano Toshie Harino Toshihito Nomura Keitaro Omori Norifumi Shigemoto Shinya Takahashi Hiroki Ohge |
| author_sort | Yuki Kaiki |
| collection | DOAJ |
| description | Abstract Background Bacteremia due to the Streptococcus bovis/Streptococcus equinus complex (SBSEC) is associated with specific diseases, such as colorectal cancer and infective endocarditis. This study aimed to evaluate the clinical characteristics of SBSEC bacteremia and the accuracy of identification of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and phenotypic identification systems for SBSEC isolates. Methods We analyzed patients with SBSEC bacteremia retrospectively between 2012 and 2019 at three hospitals in Japan. We re-identified each SBSEC isolate using sequencing superoxide dismutase (sodA) analysis, MALDI-TOF MS using the MALDI Biotyper, and phenotypic identification using the VITEK2. Results During the study period, 39 patients with SBSEC bacteremia were identified. S. gallolyticus subsp. pasteurianus (SGSP, n = 29), S. gallolyticus subsp. gallolyticus (SGSG, n = 5), S. lutetiensis (SL, n = 4), and S. infantarius subsp. infantarius (n = 1) were identified using sodA sequencing analysis. Primary bacteremia (36%) was the most common cause of bacteremia, followed by infective endocarditis (26%) and biliary tract infections (23%). Colorectal cancer was associated significantly with SGSG bacteremia, while the sources of bacteremia were similar in each SBSEC subspecies. The MALDI Biotyper was significantly more accurate in identifying the SBSEC isolates at the subspecies level compared to the VITEK2 (92% vs. 67%, P = 0.010). In contrast, there were no significant differences in the rates of correct identification of the SBSEC isolates at the species level between the MALDI Biotyper and the VITEK2 (100% vs. 87%, P = 0.055). Conclusions Bacteremia with SGSG was associated with colorectal cancer, and the sources of bacteremia were similar in each SBSEC subspecies. The MALDI-TOF MS was significantly more accurate in identifying SBSEC isolates at the subspecies level than the phenotypic identification systems. The accurate identification of SBSEC isolates using the MALDI-TOF MS and phenotypic identification systems was sufficient at the species level, but it was insufficient at the subspecies level. Therefore, it may be reasonable for clinicians to perform echocardiographies and colonoscopies in all patients with SBSEC bacteremia. |
| first_indexed | 2024-12-14T16:28:33Z |
| format | Article |
| id | doaj.art-de44d9abf5c7476885c4ca4ee447c393 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-14T16:28:33Z |
| publishDate | 2021-11-01 |
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| series | BMC Infectious Diseases |
| spelling | doaj.art-de44d9abf5c7476885c4ca4ee447c3932022-12-21T22:54:38ZengBMCBMC Infectious Diseases1471-23342021-11-012111810.1186/s12879-021-06880-4Laboratory identification and clinical characteristics of Streptococcus bovis/Streptococcus equinus complex bacteremia: a retrospective, multicenter study in Hiroshima, JapanYuki Kaiki0Hiroki Kitagawa1Kayoko Tadera2Hiroyuki Taogoshi3Mitsuyasu Ikeda4Mikihiro Kano5Toshie Harino6Toshihito Nomura7Keitaro Omori8Norifumi Shigemoto9Shinya Takahashi10Hiroki Ohge11Department of Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima UniversityDepartment of Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima UniversityProject Research Center for Nosocomial Infectious Diseases, Hiroshima UniversityDepartment of Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima UniversityProject Research Center for Nosocomial Infectious Diseases, Hiroshima UniversityDepartment of Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima UniversityDepartment of Clinical Laboratory, Hiroshima City Asa Citizens HospitalDepartment of Infectious Diseases, Hiroshima University HospitalDepartment of Infectious Diseases, Hiroshima University HospitalDepartment of Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima UniversityDepartment of Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima UniversityDepartment of Infectious Diseases, Hiroshima University HospitalAbstract Background Bacteremia due to the Streptococcus bovis/Streptococcus equinus complex (SBSEC) is associated with specific diseases, such as colorectal cancer and infective endocarditis. This study aimed to evaluate the clinical characteristics of SBSEC bacteremia and the accuracy of identification of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and phenotypic identification systems for SBSEC isolates. Methods We analyzed patients with SBSEC bacteremia retrospectively between 2012 and 2019 at three hospitals in Japan. We re-identified each SBSEC isolate using sequencing superoxide dismutase (sodA) analysis, MALDI-TOF MS using the MALDI Biotyper, and phenotypic identification using the VITEK2. Results During the study period, 39 patients with SBSEC bacteremia were identified. S. gallolyticus subsp. pasteurianus (SGSP, n = 29), S. gallolyticus subsp. gallolyticus (SGSG, n = 5), S. lutetiensis (SL, n = 4), and S. infantarius subsp. infantarius (n = 1) were identified using sodA sequencing analysis. Primary bacteremia (36%) was the most common cause of bacteremia, followed by infective endocarditis (26%) and biliary tract infections (23%). Colorectal cancer was associated significantly with SGSG bacteremia, while the sources of bacteremia were similar in each SBSEC subspecies. The MALDI Biotyper was significantly more accurate in identifying the SBSEC isolates at the subspecies level compared to the VITEK2 (92% vs. 67%, P = 0.010). In contrast, there were no significant differences in the rates of correct identification of the SBSEC isolates at the species level between the MALDI Biotyper and the VITEK2 (100% vs. 87%, P = 0.055). Conclusions Bacteremia with SGSG was associated with colorectal cancer, and the sources of bacteremia were similar in each SBSEC subspecies. The MALDI-TOF MS was significantly more accurate in identifying SBSEC isolates at the subspecies level than the phenotypic identification systems. The accurate identification of SBSEC isolates using the MALDI-TOF MS and phenotypic identification systems was sufficient at the species level, but it was insufficient at the subspecies level. Therefore, it may be reasonable for clinicians to perform echocardiographies and colonoscopies in all patients with SBSEC bacteremia.https://doi.org/10.1186/s12879-021-06880-4Streptococcus bovisBloodstream infectionMALDI-TOF MSsodAColorectal cancerInfective endocarditis |
| spellingShingle | Yuki Kaiki Hiroki Kitagawa Kayoko Tadera Hiroyuki Taogoshi Mitsuyasu Ikeda Mikihiro Kano Toshie Harino Toshihito Nomura Keitaro Omori Norifumi Shigemoto Shinya Takahashi Hiroki Ohge Laboratory identification and clinical characteristics of Streptococcus bovis/Streptococcus equinus complex bacteremia: a retrospective, multicenter study in Hiroshima, Japan BMC Infectious Diseases Streptococcus bovis Bloodstream infection MALDI-TOF MS sodA Colorectal cancer Infective endocarditis |
| title | Laboratory identification and clinical characteristics of Streptococcus bovis/Streptococcus equinus complex bacteremia: a retrospective, multicenter study in Hiroshima, Japan |
| title_full | Laboratory identification and clinical characteristics of Streptococcus bovis/Streptococcus equinus complex bacteremia: a retrospective, multicenter study in Hiroshima, Japan |
| title_fullStr | Laboratory identification and clinical characteristics of Streptococcus bovis/Streptococcus equinus complex bacteremia: a retrospective, multicenter study in Hiroshima, Japan |
| title_full_unstemmed | Laboratory identification and clinical characteristics of Streptococcus bovis/Streptococcus equinus complex bacteremia: a retrospective, multicenter study in Hiroshima, Japan |
| title_short | Laboratory identification and clinical characteristics of Streptococcus bovis/Streptococcus equinus complex bacteremia: a retrospective, multicenter study in Hiroshima, Japan |
| title_sort | laboratory identification and clinical characteristics of streptococcus bovis streptococcus equinus complex bacteremia a retrospective multicenter study in hiroshima japan |
| topic | Streptococcus bovis Bloodstream infection MALDI-TOF MS sodA Colorectal cancer Infective endocarditis |
| url | https://doi.org/10.1186/s12879-021-06880-4 |
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