Arabidopsis microRNA expression regulation in a wide range of abiotic stress responses
Arabidopsis microRNA expression regulation was studied in a wide array of abiotic stresses such as drought, heat, salinity, copper excess/deficiency, cadmium excess and sulphur deficiency. A home-built RT-qPCR mirEX platform for the amplification of 289 Arabidopsis microRNA transcripts was used to s...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2015-06-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00410/full |
| _version_ | 1818134493133799424 |
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| author | Maria eBarciszewska-Pacak Kaja eMilanowska Katarzyna eKnop Dawid eBielewicz Przemyslaw eNuc Patrycja ePlewka Andrzej Miroslaw Pacak Franck eVazquez Wojciech eKarlowski Artur eJarmolowski Zofia eSzweykowska-Kulinska |
| author_facet | Maria eBarciszewska-Pacak Kaja eMilanowska Katarzyna eKnop Dawid eBielewicz Przemyslaw eNuc Patrycja ePlewka Andrzej Miroslaw Pacak Franck eVazquez Wojciech eKarlowski Artur eJarmolowski Zofia eSzweykowska-Kulinska |
| author_sort | Maria eBarciszewska-Pacak |
| collection | DOAJ |
| description | Arabidopsis microRNA expression regulation was studied in a wide array of abiotic stresses such as drought, heat, salinity, copper excess/deficiency, cadmium excess and sulphur deficiency. A home-built RT-qPCR mirEX platform for the amplification of 289 Arabidopsis microRNA transcripts was used to study their response to abiotic stresses. Small RNA sequencing, Northern hybridization and TaqMan® microRNA assays were performed to study the abundance of mature microRNAs. A broad response on the level of primary miRNAs (pri-miRNAs) was observed. However, stress response at the level of mature microRNAs was rather confined. The data presented show that in most instances, the level of a particular mature miRNA could not be predicted based on the level of its pri-miRNA. This points to an essential role of posttranscriptional regulation of microRNA expression. New Arabidopsis microRNAs responsive to abiotic stresses were discovered. Four microRNAs: miR319a/b, miR319b.2, and miR400 have been found to be responsive to several abiotic stresses and thus can be regarded as general stress-responsive microRNA species. |
| first_indexed | 2024-12-11T09:09:29Z |
| format | Article |
| id | doaj.art-de4e17e5a7834051a7ed4a81d67030a0 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-11T09:09:29Z |
| publishDate | 2015-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-de4e17e5a7834051a7ed4a81d67030a02022-12-22T01:13:33ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2015-06-01610.3389/fpls.2015.00410141513Arabidopsis microRNA expression regulation in a wide range of abiotic stress responsesMaria eBarciszewska-Pacak0Kaja eMilanowska1Katarzyna eKnop2Dawid eBielewicz3Przemyslaw eNuc4Patrycja ePlewka5Andrzej Miroslaw Pacak6Franck eVazquez7Wojciech eKarlowski8Artur eJarmolowski9Zofia eSzweykowska-Kulinska10Adam Mickiewicz UniversityAdam Mickiewicz UniversityAdam Mickiewicz UniversityAdam Mickiewicz UniversityAdam Mickiewicz UniversityAdam Mickiewicz UniversityAdam Mickiewicz UniversityUniversity of BaselAdam Mickiewicz UniversityAdam Mickiewicz UniversityAdam Mickiewicz UniversityArabidopsis microRNA expression regulation was studied in a wide array of abiotic stresses such as drought, heat, salinity, copper excess/deficiency, cadmium excess and sulphur deficiency. A home-built RT-qPCR mirEX platform for the amplification of 289 Arabidopsis microRNA transcripts was used to study their response to abiotic stresses. Small RNA sequencing, Northern hybridization and TaqMan® microRNA assays were performed to study the abundance of mature microRNAs. A broad response on the level of primary miRNAs (pri-miRNAs) was observed. However, stress response at the level of mature microRNAs was rather confined. The data presented show that in most instances, the level of a particular mature miRNA could not be predicted based on the level of its pri-miRNA. This points to an essential role of posttranscriptional regulation of microRNA expression. New Arabidopsis microRNAs responsive to abiotic stresses were discovered. Four microRNAs: miR319a/b, miR319b.2, and miR400 have been found to be responsive to several abiotic stresses and thus can be regarded as general stress-responsive microRNA species.http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00410/fullGene ExpressionmiRNAabiotic stresspri-miRNAmiRNA expression regulation |
| spellingShingle | Maria eBarciszewska-Pacak Kaja eMilanowska Katarzyna eKnop Dawid eBielewicz Przemyslaw eNuc Patrycja ePlewka Andrzej Miroslaw Pacak Franck eVazquez Wojciech eKarlowski Artur eJarmolowski Zofia eSzweykowska-Kulinska Arabidopsis microRNA expression regulation in a wide range of abiotic stress responses Frontiers in Plant Science Gene Expression miRNA abiotic stress pri-miRNA miRNA expression regulation |
| title | Arabidopsis microRNA expression regulation in a wide range of abiotic stress responses |
| title_full | Arabidopsis microRNA expression regulation in a wide range of abiotic stress responses |
| title_fullStr | Arabidopsis microRNA expression regulation in a wide range of abiotic stress responses |
| title_full_unstemmed | Arabidopsis microRNA expression regulation in a wide range of abiotic stress responses |
| title_short | Arabidopsis microRNA expression regulation in a wide range of abiotic stress responses |
| title_sort | arabidopsis microrna expression regulation in a wide range of abiotic stress responses |
| topic | Gene Expression miRNA abiotic stress pri-miRNA miRNA expression regulation |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00410/full |
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