Optimization of Enzymatic-assisted Extraction of Polysaccharides from Roxburgh Rose Pomace and its Antioxidant Activity
In this experiment, at first the roxburgh rose juice was extracted and then roxburgh rose residue was taken as raw material. We have used the enzyme assisted method to study the extraction process of polysaccharides from roxburgh rose pomace. The effects of mesh number, the concentration of the enzy...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
EDP Sciences
2019-01-01
|
| Series: | E3S Web of Conferences |
| Online Access: | https://doi.org/10.1051/e3sconf/20197802014 |
| _version_ | 1818664244041744384 |
|---|---|
| author | Zhou Xiaoli Zhu Guangxu Yang Yansheng Du Bin Lin Dong |
| author_facet | Zhou Xiaoli Zhu Guangxu Yang Yansheng Du Bin Lin Dong |
| author_sort | Zhou Xiaoli |
| collection | DOAJ |
| description | In this experiment, at first the roxburgh rose juice was extracted and then roxburgh rose residue was taken as raw material. We have used the enzyme assisted method to study the extraction process of polysaccharides from roxburgh rose pomace. The effects of mesh number, the concentration of the enzyme, temperature and time, pH and solid-liquid ratio on the polysaccharides yield were explored by single factor experiments. And then orthogonal experiment was designed to study the optimal techniques on extracting of polysaccharides from pomace. The in vitro antioxidant activity of the obtained polysaccharides was studied. The results showed that the optimal condition of extracting polysaccharides was as follows: enzyme concentration 2.5%, enzymatic hydrolysis at 60° for 40 min, pH 4.0, mesh number were 100 and solidliquid ratio was 1:25 based on the ratio of cellulase and pectinase was 2: 1. The average polysaccharides yield of enzymatic extraction method reached (4.79±0.07) % under the optimal condition. The antioxidant activity assays in vitro revealed that polysaccharides from roxburgh rose pomace can be used as natural antioxidants in functional foods and pharmaceutical industries. |
| first_indexed | 2024-12-17T05:29:39Z |
| format | Article |
| id | doaj.art-de519dc14a00444ab76c787bcec85c37 |
| institution | Directory Open Access Journal |
| issn | 2267-1242 |
| language | English |
| last_indexed | 2024-12-17T05:29:39Z |
| publishDate | 2019-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj.art-de519dc14a00444ab76c787bcec85c372022-12-21T22:01:45ZengEDP SciencesE3S Web of Conferences2267-12422019-01-01780201410.1051/e3sconf/20197802014e3sconf_fsee2018_02014Optimization of Enzymatic-assisted Extraction of Polysaccharides from Roxburgh Rose Pomace and its Antioxidant ActivityZhou XiaoliZhu GuangxuYang YanshengDu BinLin DongIn this experiment, at first the roxburgh rose juice was extracted and then roxburgh rose residue was taken as raw material. We have used the enzyme assisted method to study the extraction process of polysaccharides from roxburgh rose pomace. The effects of mesh number, the concentration of the enzyme, temperature and time, pH and solid-liquid ratio on the polysaccharides yield were explored by single factor experiments. And then orthogonal experiment was designed to study the optimal techniques on extracting of polysaccharides from pomace. The in vitro antioxidant activity of the obtained polysaccharides was studied. The results showed that the optimal condition of extracting polysaccharides was as follows: enzyme concentration 2.5%, enzymatic hydrolysis at 60° for 40 min, pH 4.0, mesh number were 100 and solidliquid ratio was 1:25 based on the ratio of cellulase and pectinase was 2: 1. The average polysaccharides yield of enzymatic extraction method reached (4.79±0.07) % under the optimal condition. The antioxidant activity assays in vitro revealed that polysaccharides from roxburgh rose pomace can be used as natural antioxidants in functional foods and pharmaceutical industries.https://doi.org/10.1051/e3sconf/20197802014 |
| spellingShingle | Zhou Xiaoli Zhu Guangxu Yang Yansheng Du Bin Lin Dong Optimization of Enzymatic-assisted Extraction of Polysaccharides from Roxburgh Rose Pomace and its Antioxidant Activity E3S Web of Conferences |
| title | Optimization of Enzymatic-assisted Extraction of Polysaccharides from Roxburgh Rose Pomace and its Antioxidant Activity |
| title_full | Optimization of Enzymatic-assisted Extraction of Polysaccharides from Roxburgh Rose Pomace and its Antioxidant Activity |
| title_fullStr | Optimization of Enzymatic-assisted Extraction of Polysaccharides from Roxburgh Rose Pomace and its Antioxidant Activity |
| title_full_unstemmed | Optimization of Enzymatic-assisted Extraction of Polysaccharides from Roxburgh Rose Pomace and its Antioxidant Activity |
| title_short | Optimization of Enzymatic-assisted Extraction of Polysaccharides from Roxburgh Rose Pomace and its Antioxidant Activity |
| title_sort | optimization of enzymatic assisted extraction of polysaccharides from roxburgh rose pomace and its antioxidant activity |
| url | https://doi.org/10.1051/e3sconf/20197802014 |
| work_keys_str_mv | AT zhouxiaoli optimizationofenzymaticassistedextractionofpolysaccharidesfromroxburghrosepomaceanditsantioxidantactivity AT zhuguangxu optimizationofenzymaticassistedextractionofpolysaccharidesfromroxburghrosepomaceanditsantioxidantactivity AT yangyansheng optimizationofenzymaticassistedextractionofpolysaccharidesfromroxburghrosepomaceanditsantioxidantactivity AT dubin optimizationofenzymaticassistedextractionofpolysaccharidesfromroxburghrosepomaceanditsantioxidantactivity AT lindong optimizationofenzymaticassistedextractionofpolysaccharidesfromroxburghrosepomaceanditsantioxidantactivity |