Effect of maize root exudates on indole-3-acetic acid production by rice endophytic bacteria under influence of L-tryptophan [version 1; referees: 2 approved]
Background: It is assumed that plant growth regulators produced by beneficial bacterial species could also influence plant growth. IAA is a major plant growth regulator responsible for stimulation of plant growth. There are several microorganisms which are naturally responsible for L- tryptophan met...
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| Format: | Article |
| Language: | English |
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F1000 Research Ltd
2018-01-01
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| Series: | F1000Research |
| Online Access: | https://f1000research.com/articles/7-112/v1 |
| _version_ | 1818277466881392640 |
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| author | Arun Karnwal Aradhana Dohroo |
| author_facet | Arun Karnwal Aradhana Dohroo |
| author_sort | Arun Karnwal |
| collection | DOAJ |
| description | Background: It is assumed that plant growth regulators produced by beneficial bacterial species could also influence plant growth. IAA is a major plant growth regulator responsible for stimulation of plant growth. There are several microorganisms which are naturally responsible for L- tryptophan metabolism. Methods: In total, 56 indigenous morphologically distinct isolates from rice roots were selected and subsequently characterized with biochemical tests, 16S rRNA sequencing and plant growth promoting activities. Pseudomonas fluorescens RE1 (GenBank: MF102882.1) and RE17 (GenBank: MF103672.1) endophytes resulted in better PGP activity against the other 54 isolates. Both endophytes were tested to screen indole-3-acetic acid production ability in pure culture conditions with L-tryptophan at 0, 50, 100, 200 and 500µg/ml concentrations. Results: P. fluorescens RE1 was recorded efficient for indole production in comparison to P. fluorescens RE17 at various L-tryptophan concentrations. P. fluorescens RE1 was shown to produce between 0.8 µg/ml and 11.5µg/ml of indole at various tryptophan concentrations, while RE17 produced between 1.2µg/ml and 10.2µg/ml. At 200 and 500µg/ml tryptophan concentration, P. fluorescens RE17 produced 7.4pmol/ml and 9.3pmol/ml IAA, respectively. Conclusions: Inoculation of maize seed with P. fluorescens RE1 and RE17 showed a significantly higher level of IAA production in comparison to non-inoculated seeds. Current study outcomes proved that plant growth regulators produced by Pseudomonas species could also play a critical role in plant growth promotion. |
| first_indexed | 2024-12-12T23:02:00Z |
| format | Article |
| id | doaj.art-de51d32aee0444aaa6f0236261f00611 |
| institution | Directory Open Access Journal |
| issn | 2046-1402 |
| language | English |
| last_indexed | 2024-12-12T23:02:00Z |
| publishDate | 2018-01-01 |
| publisher | F1000 Research Ltd |
| record_format | Article |
| series | F1000Research |
| spelling | doaj.art-de51d32aee0444aaa6f0236261f006112022-12-22T00:08:48ZengF1000 Research LtdF1000Research2046-14022018-01-01710.12688/f1000research.13644.114823Effect of maize root exudates on indole-3-acetic acid production by rice endophytic bacteria under influence of L-tryptophan [version 1; referees: 2 approved]Arun Karnwal0Aradhana Dohroo1School of Bioengineering and Biosciences, Lovely Professional University, Jalandhar, Punjab, IndiaBhojia Institute Of Life Sciences, Budh (Baddi), Solan, Himachal Pradesh, IndiaBackground: It is assumed that plant growth regulators produced by beneficial bacterial species could also influence plant growth. IAA is a major plant growth regulator responsible for stimulation of plant growth. There are several microorganisms which are naturally responsible for L- tryptophan metabolism. Methods: In total, 56 indigenous morphologically distinct isolates from rice roots were selected and subsequently characterized with biochemical tests, 16S rRNA sequencing and plant growth promoting activities. Pseudomonas fluorescens RE1 (GenBank: MF102882.1) and RE17 (GenBank: MF103672.1) endophytes resulted in better PGP activity against the other 54 isolates. Both endophytes were tested to screen indole-3-acetic acid production ability in pure culture conditions with L-tryptophan at 0, 50, 100, 200 and 500µg/ml concentrations. Results: P. fluorescens RE1 was recorded efficient for indole production in comparison to P. fluorescens RE17 at various L-tryptophan concentrations. P. fluorescens RE1 was shown to produce between 0.8 µg/ml and 11.5µg/ml of indole at various tryptophan concentrations, while RE17 produced between 1.2µg/ml and 10.2µg/ml. At 200 and 500µg/ml tryptophan concentration, P. fluorescens RE17 produced 7.4pmol/ml and 9.3pmol/ml IAA, respectively. Conclusions: Inoculation of maize seed with P. fluorescens RE1 and RE17 showed a significantly higher level of IAA production in comparison to non-inoculated seeds. Current study outcomes proved that plant growth regulators produced by Pseudomonas species could also play a critical role in plant growth promotion.https://f1000research.com/articles/7-112/v1 |
| spellingShingle | Arun Karnwal Aradhana Dohroo Effect of maize root exudates on indole-3-acetic acid production by rice endophytic bacteria under influence of L-tryptophan [version 1; referees: 2 approved] F1000Research |
| title | Effect of maize root exudates on indole-3-acetic acid production by rice endophytic bacteria under influence of L-tryptophan [version 1; referees: 2 approved] |
| title_full | Effect of maize root exudates on indole-3-acetic acid production by rice endophytic bacteria under influence of L-tryptophan [version 1; referees: 2 approved] |
| title_fullStr | Effect of maize root exudates on indole-3-acetic acid production by rice endophytic bacteria under influence of L-tryptophan [version 1; referees: 2 approved] |
| title_full_unstemmed | Effect of maize root exudates on indole-3-acetic acid production by rice endophytic bacteria under influence of L-tryptophan [version 1; referees: 2 approved] |
| title_short | Effect of maize root exudates on indole-3-acetic acid production by rice endophytic bacteria under influence of L-tryptophan [version 1; referees: 2 approved] |
| title_sort | effect of maize root exudates on indole 3 acetic acid production by rice endophytic bacteria under influence of l tryptophan version 1 referees 2 approved |
| url | https://f1000research.com/articles/7-112/v1 |
| work_keys_str_mv | AT arunkarnwal effectofmaizerootexudatesonindole3aceticacidproductionbyriceendophyticbacteriaunderinfluenceofltryptophanversion1referees2approved AT aradhanadohroo effectofmaizerootexudatesonindole3aceticacidproductionbyriceendophyticbacteriaunderinfluenceofltryptophanversion1referees2approved |