Clade 2.3.4.4b H5N8 Subtype Avian Influenza Viruses Were Identified from the Common Crane Wintering in Yunnan Province, China
The seasonal migration of wild aquatic birds plays a critical role in the maintenance, transmission, and incursion of the avian influenza virus (AIV). AIV surveillance was performed during 2020–2021 in two national nature reserves with abundant wild bird resources in Yunnan, China. Four H5N8 AIVs is...
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MDPI AG
2022-12-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/1/38 |
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| author | Qinhong Yang Xiaoyan Xue Zhenxing Zhang Ming J. Wu Jia Ji Wei Wang Hongbin Yin Suhua Li Hongyang Dai Bofang Duan Qiang Liu Jianling Song |
| author_facet | Qinhong Yang Xiaoyan Xue Zhenxing Zhang Ming J. Wu Jia Ji Wei Wang Hongbin Yin Suhua Li Hongyang Dai Bofang Duan Qiang Liu Jianling Song |
| author_sort | Qinhong Yang |
| collection | DOAJ |
| description | The seasonal migration of wild aquatic birds plays a critical role in the maintenance, transmission, and incursion of the avian influenza virus (AIV). AIV surveillance was performed during 2020–2021 in two national nature reserves with abundant wild bird resources in Yunnan, China. Four H5N8 AIVs isolates from the common crane were identified by next-generation sequencing. Phylogenetic analysis demonstrated that all eight gene segments of these H5N8 AIVs belonged to clade 2.3.4.4b high-pathogenic AIV (HPAIV) and shared high nucleotide sequence similarity with the strains isolated in Hubei, China, and Siberia, Russia, in 2020–2021. The H5N8 HPAIVs from common cranes were characterized by both human and avian dual-receptor specificity in the hemagglutinin (HA) protein. Moreover, possessing the substitutions contributes to overcoming transmission barriers of mammalian hosts in polymerase basic 2 (PB2), polymerase basic protein 1 (PB1), and polymerase acid (PA), and exhibiting the long stalk in the neck region of the neuraminidase (NA) protein contributes to adaptation in wild birds. Monitoring AIVs in migratory birds, at stopover sites and in their primary habitats, i.e., breeding or wintering grounds, could provide insight into potential zoonosis caused by AIVs. |
| first_indexed | 2024-03-09T11:03:39Z |
| format | Article |
| id | doaj.art-de55701434144f74ba3fe92740a7ebd8 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T11:03:39Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-de55701434144f74ba3fe92740a7ebd82023-12-01T01:06:55ZengMDPI AGViruses1999-49152022-12-011513810.3390/v15010038Clade 2.3.4.4b H5N8 Subtype Avian Influenza Viruses Were Identified from the Common Crane Wintering in Yunnan Province, ChinaQinhong Yang0Xiaoyan Xue1Zhenxing Zhang2Ming J. Wu3Jia Ji4Wei Wang5Hongbin Yin6Suhua Li7Hongyang Dai8Bofang Duan9Qiang Liu10Jianling Song11College of Life Sciences, Southwest Forestry University, 300 Bailong Road, Kunming 650024, ChinaCollege of Life Sciences, Southwest Forestry University, 300 Bailong Road, Kunming 650024, ChinaYunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Academy of Animal Husbandry and Veterinary, 6 Qinglongshan, Kunming 650224, ChinaSchool of Science, Western Sydney University, Locked Bag 1797, Penrith, NSW 2751, AustraliaCollege of Life Sciences, Southwest Forestry University, 300 Bailong Road, Kunming 650024, ChinaCollege of Life Sciences, Southwest Forestry University, 300 Bailong Road, Kunming 650024, ChinaAnimal Disease Inspection and Supervision Institution of Yunnan Province, 118 Gulou Road, Kunming 650051, ChinaCollege of Life Sciences, Southwest Forestry University, 300 Bailong Road, Kunming 650024, ChinaThe Management Bureau of Huize Black—Necked Crane National Nature Reserve, 744 Tongbao Road, Qujing 654200, ChinaYunnan Center for Animal Disease Control and Prevention, 95 Jinhei Road, Kunming 650034, ChinaCollege of Life Sciences, Southwest Forestry University, 300 Bailong Road, Kunming 650024, ChinaYunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Academy of Animal Husbandry and Veterinary, 6 Qinglongshan, Kunming 650224, ChinaThe seasonal migration of wild aquatic birds plays a critical role in the maintenance, transmission, and incursion of the avian influenza virus (AIV). AIV surveillance was performed during 2020–2021 in two national nature reserves with abundant wild bird resources in Yunnan, China. Four H5N8 AIVs isolates from the common crane were identified by next-generation sequencing. Phylogenetic analysis demonstrated that all eight gene segments of these H5N8 AIVs belonged to clade 2.3.4.4b high-pathogenic AIV (HPAIV) and shared high nucleotide sequence similarity with the strains isolated in Hubei, China, and Siberia, Russia, in 2020–2021. The H5N8 HPAIVs from common cranes were characterized by both human and avian dual-receptor specificity in the hemagglutinin (HA) protein. Moreover, possessing the substitutions contributes to overcoming transmission barriers of mammalian hosts in polymerase basic 2 (PB2), polymerase basic protein 1 (PB1), and polymerase acid (PA), and exhibiting the long stalk in the neck region of the neuraminidase (NA) protein contributes to adaptation in wild birds. Monitoring AIVs in migratory birds, at stopover sites and in their primary habitats, i.e., breeding or wintering grounds, could provide insight into potential zoonosis caused by AIVs.https://www.mdpi.com/1999-4915/15/1/38avian influenza virusH5N8wild birdgenetic analysis |
| spellingShingle | Qinhong Yang Xiaoyan Xue Zhenxing Zhang Ming J. Wu Jia Ji Wei Wang Hongbin Yin Suhua Li Hongyang Dai Bofang Duan Qiang Liu Jianling Song Clade 2.3.4.4b H5N8 Subtype Avian Influenza Viruses Were Identified from the Common Crane Wintering in Yunnan Province, China Viruses avian influenza virus H5N8 wild bird genetic analysis |
| title | Clade 2.3.4.4b H5N8 Subtype Avian Influenza Viruses Were Identified from the Common Crane Wintering in Yunnan Province, China |
| title_full | Clade 2.3.4.4b H5N8 Subtype Avian Influenza Viruses Were Identified from the Common Crane Wintering in Yunnan Province, China |
| title_fullStr | Clade 2.3.4.4b H5N8 Subtype Avian Influenza Viruses Were Identified from the Common Crane Wintering in Yunnan Province, China |
| title_full_unstemmed | Clade 2.3.4.4b H5N8 Subtype Avian Influenza Viruses Were Identified from the Common Crane Wintering in Yunnan Province, China |
| title_short | Clade 2.3.4.4b H5N8 Subtype Avian Influenza Viruses Were Identified from the Common Crane Wintering in Yunnan Province, China |
| title_sort | clade 2 3 4 4b h5n8 subtype avian influenza viruses were identified from the common crane wintering in yunnan province china |
| topic | avian influenza virus H5N8 wild bird genetic analysis |
| url | https://www.mdpi.com/1999-4915/15/1/38 |
| work_keys_str_mv | AT qinhongyang clade2344bh5n8subtypeavianinfluenzaviruseswereidentifiedfromthecommoncranewinteringinyunnanprovincechina AT xiaoyanxue clade2344bh5n8subtypeavianinfluenzaviruseswereidentifiedfromthecommoncranewinteringinyunnanprovincechina AT zhenxingzhang clade2344bh5n8subtypeavianinfluenzaviruseswereidentifiedfromthecommoncranewinteringinyunnanprovincechina AT mingjwu clade2344bh5n8subtypeavianinfluenzaviruseswereidentifiedfromthecommoncranewinteringinyunnanprovincechina AT jiaji clade2344bh5n8subtypeavianinfluenzaviruseswereidentifiedfromthecommoncranewinteringinyunnanprovincechina AT weiwang clade2344bh5n8subtypeavianinfluenzaviruseswereidentifiedfromthecommoncranewinteringinyunnanprovincechina AT hongbinyin clade2344bh5n8subtypeavianinfluenzaviruseswereidentifiedfromthecommoncranewinteringinyunnanprovincechina AT suhuali clade2344bh5n8subtypeavianinfluenzaviruseswereidentifiedfromthecommoncranewinteringinyunnanprovincechina AT hongyangdai clade2344bh5n8subtypeavianinfluenzaviruseswereidentifiedfromthecommoncranewinteringinyunnanprovincechina AT bofangduan clade2344bh5n8subtypeavianinfluenzaviruseswereidentifiedfromthecommoncranewinteringinyunnanprovincechina AT qiangliu clade2344bh5n8subtypeavianinfluenzaviruseswereidentifiedfromthecommoncranewinteringinyunnanprovincechina AT jianlingsong clade2344bh5n8subtypeavianinfluenzaviruseswereidentifiedfromthecommoncranewinteringinyunnanprovincechina |