New species based on the biological species concept within the complex of Lariophagus distinguendus (Hymenoptera, Chalcidoidea, Pteromalidae), a parasitoid of household pests
Abstract The pteromalid parasitoid Lariophagus distinguendus (Foerster) belongs to the Hymenoptera, a megadiverse insect order with high cryptic diversity. It attacks stored product pest beetles in human storage facilities. Recently, it has been shown to consist of two separate species. To further s...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-09-01
|
| Series: | Ecology and Evolution |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ece3.10524 |
| _version_ | 1827698801897373696 |
|---|---|
| author | Marie Pollmann Denise Kuhn Christian König Irmela Homolka Sina Paschke Ronja Reinisch Anna Schmidt Noa Schwabe Justus Weber Yuval Gottlieb Johannes Luitpold Maria Steidle |
| author_facet | Marie Pollmann Denise Kuhn Christian König Irmela Homolka Sina Paschke Ronja Reinisch Anna Schmidt Noa Schwabe Justus Weber Yuval Gottlieb Johannes Luitpold Maria Steidle |
| author_sort | Marie Pollmann |
| collection | DOAJ |
| description | Abstract The pteromalid parasitoid Lariophagus distinguendus (Foerster) belongs to the Hymenoptera, a megadiverse insect order with high cryptic diversity. It attacks stored product pest beetles in human storage facilities. Recently, it has been shown to consist of two separate species. To further study its cryptic diversity, strains were collected to compare their relatedness using barcoding and nuclear genes. Nuclear genes identified two clusters which agree with the known two species, whereas the barcode fragment determined an additional third Clade. Total reproductive isolation (RI) according to the biological species concept (BSC) was investigated in crossing experiments within and between clusters using representative strains. Sexual isolation exists between all studied pairs, increasing from slight to strong with genetic distance. Postzygotic barriers mostly affected hybrid males, pointing to Haldane's rule. Hybrid females were only affected by unidirectional Spiroplasma‐induced cytoplasmic incompatibility and behavioural sterility, each in one specific strain combination. RI was virtually absent between strains separated by up to 2.8% COI difference, but strong or complete in three pairs from one Clade each, separated by at least 7.2%. Apparently, each of these clusters represents one separate species according to the BSC, highlighting cryptic diversity in direct vicinity to humans. In addition, these results challenge the recent ‘turbo‐taxonomy’ practice of using 2% COI differences to delimitate species, especially within parasitic Hymenoptera. The gradual increase in number and strength of reproductive barriers between strains with increasing genetic distance also sheds light on the emergence of barriers during the speciation process in L. distinguendus. |
| first_indexed | 2024-03-10T13:37:13Z |
| format | Article |
| id | doaj.art-de5797580cca4cfc9a16a92dbaff3fb9 |
| institution | Directory Open Access Journal |
| issn | 2045-7758 |
| language | English |
| last_indexed | 2024-03-10T13:37:13Z |
| publishDate | 2023-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Ecology and Evolution |
| spelling | doaj.art-de5797580cca4cfc9a16a92dbaff3fb92023-11-21T07:26:25ZengWileyEcology and Evolution2045-77582023-09-01139n/an/a10.1002/ece3.10524New species based on the biological species concept within the complex of Lariophagus distinguendus (Hymenoptera, Chalcidoidea, Pteromalidae), a parasitoid of household pestsMarie Pollmann0Denise Kuhn1Christian König2Irmela Homolka3Sina Paschke4Ronja Reinisch5Anna Schmidt6Noa Schwabe7Justus Weber8Yuval Gottlieb9Johannes Luitpold Maria Steidle10Department of Chemical Ecology 190t, Institute of Biology University of Hohenheim Stuttgart GermanyDepartment of Entomology 360c, Institute of Phytomedicine University of Hohenheim Stuttgart GermanyAkademie für Natur‐ und Umweltschutz Baden‐Württemberg Stuttgart GermanyDepartment of Chemical Ecology 190t, Institute of Biology University of Hohenheim Stuttgart GermanyDepartment of Chemical Ecology 190t, Institute of Biology University of Hohenheim Stuttgart GermanyDepartment of Chemical Ecology 190t, Institute of Biology University of Hohenheim Stuttgart GermanyDepartment of Chemical Ecology 190t, Institute of Biology University of Hohenheim Stuttgart GermanyPlant Evolutionary Biology 190b, Institute of Biology University of Hohenheim Stuttgart GermanyDepartment of Chemical Ecology 190t, Institute of Biology University of Hohenheim Stuttgart GermanyRobert H. Smith Faculty of Agriculture, Food and Environment, Koret School of Veterinary Medicine Hebrew University of Jerusalem Rehovot IsraelDepartment of Chemical Ecology 190t, Institute of Biology University of Hohenheim Stuttgart GermanyAbstract The pteromalid parasitoid Lariophagus distinguendus (Foerster) belongs to the Hymenoptera, a megadiverse insect order with high cryptic diversity. It attacks stored product pest beetles in human storage facilities. Recently, it has been shown to consist of two separate species. To further study its cryptic diversity, strains were collected to compare their relatedness using barcoding and nuclear genes. Nuclear genes identified two clusters which agree with the known two species, whereas the barcode fragment determined an additional third Clade. Total reproductive isolation (RI) according to the biological species concept (BSC) was investigated in crossing experiments within and between clusters using representative strains. Sexual isolation exists between all studied pairs, increasing from slight to strong with genetic distance. Postzygotic barriers mostly affected hybrid males, pointing to Haldane's rule. Hybrid females were only affected by unidirectional Spiroplasma‐induced cytoplasmic incompatibility and behavioural sterility, each in one specific strain combination. RI was virtually absent between strains separated by up to 2.8% COI difference, but strong or complete in three pairs from one Clade each, separated by at least 7.2%. Apparently, each of these clusters represents one separate species according to the BSC, highlighting cryptic diversity in direct vicinity to humans. In addition, these results challenge the recent ‘turbo‐taxonomy’ practice of using 2% COI differences to delimitate species, especially within parasitic Hymenoptera. The gradual increase in number and strength of reproductive barriers between strains with increasing genetic distance also sheds light on the emergence of barriers during the speciation process in L. distinguendus.https://doi.org/10.1002/ece3.10524cryptic diversityparasitoid waspsreproductive isolationspecies delimitationturbo‐taxonomy |
| spellingShingle | Marie Pollmann Denise Kuhn Christian König Irmela Homolka Sina Paschke Ronja Reinisch Anna Schmidt Noa Schwabe Justus Weber Yuval Gottlieb Johannes Luitpold Maria Steidle New species based on the biological species concept within the complex of Lariophagus distinguendus (Hymenoptera, Chalcidoidea, Pteromalidae), a parasitoid of household pests Ecology and Evolution cryptic diversity parasitoid wasps reproductive isolation species delimitation turbo‐taxonomy |
| title | New species based on the biological species concept within the complex of Lariophagus distinguendus (Hymenoptera, Chalcidoidea, Pteromalidae), a parasitoid of household pests |
| title_full | New species based on the biological species concept within the complex of Lariophagus distinguendus (Hymenoptera, Chalcidoidea, Pteromalidae), a parasitoid of household pests |
| title_fullStr | New species based on the biological species concept within the complex of Lariophagus distinguendus (Hymenoptera, Chalcidoidea, Pteromalidae), a parasitoid of household pests |
| title_full_unstemmed | New species based on the biological species concept within the complex of Lariophagus distinguendus (Hymenoptera, Chalcidoidea, Pteromalidae), a parasitoid of household pests |
| title_short | New species based on the biological species concept within the complex of Lariophagus distinguendus (Hymenoptera, Chalcidoidea, Pteromalidae), a parasitoid of household pests |
| title_sort | new species based on the biological species concept within the complex of lariophagus distinguendus hymenoptera chalcidoidea pteromalidae a parasitoid of household pests |
| topic | cryptic diversity parasitoid wasps reproductive isolation species delimitation turbo‐taxonomy |
| url | https://doi.org/10.1002/ece3.10524 |
| work_keys_str_mv | AT mariepollmann newspeciesbasedonthebiologicalspeciesconceptwithinthecomplexoflariophagusdistinguendushymenopterachalcidoideapteromalidaeaparasitoidofhouseholdpests AT denisekuhn newspeciesbasedonthebiologicalspeciesconceptwithinthecomplexoflariophagusdistinguendushymenopterachalcidoideapteromalidaeaparasitoidofhouseholdpests AT christiankonig newspeciesbasedonthebiologicalspeciesconceptwithinthecomplexoflariophagusdistinguendushymenopterachalcidoideapteromalidaeaparasitoidofhouseholdpests AT irmelahomolka newspeciesbasedonthebiologicalspeciesconceptwithinthecomplexoflariophagusdistinguendushymenopterachalcidoideapteromalidaeaparasitoidofhouseholdpests AT sinapaschke newspeciesbasedonthebiologicalspeciesconceptwithinthecomplexoflariophagusdistinguendushymenopterachalcidoideapteromalidaeaparasitoidofhouseholdpests AT ronjareinisch newspeciesbasedonthebiologicalspeciesconceptwithinthecomplexoflariophagusdistinguendushymenopterachalcidoideapteromalidaeaparasitoidofhouseholdpests AT annaschmidt newspeciesbasedonthebiologicalspeciesconceptwithinthecomplexoflariophagusdistinguendushymenopterachalcidoideapteromalidaeaparasitoidofhouseholdpests AT noaschwabe newspeciesbasedonthebiologicalspeciesconceptwithinthecomplexoflariophagusdistinguendushymenopterachalcidoideapteromalidaeaparasitoidofhouseholdpests AT justusweber newspeciesbasedonthebiologicalspeciesconceptwithinthecomplexoflariophagusdistinguendushymenopterachalcidoideapteromalidaeaparasitoidofhouseholdpests AT yuvalgottlieb newspeciesbasedonthebiologicalspeciesconceptwithinthecomplexoflariophagusdistinguendushymenopterachalcidoideapteromalidaeaparasitoidofhouseholdpests AT johannesluitpoldmariasteidle newspeciesbasedonthebiologicalspeciesconceptwithinthecomplexoflariophagusdistinguendushymenopterachalcidoideapteromalidaeaparasitoidofhouseholdpests |