Dopaminergic signaling within the primary cilia in the renovascular system
Activation of dopamine receptor type-5 (DR5) has been known to reduce systemic blood pressure, most likely by increasing renal vasodilation and enhancing natriuresis in the kidney. However, the mechanism of DR5 in natriuresis and vasodilation was not clearly known. We have previously shown that DR...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-04-01
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| Series: | Frontiers in Physiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fphys.2015.00103/full |
| _version_ | 1818621561435848704 |
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| author | Kimberly Fisher Atkinson Sarmed eKathem Xingjian eJin Brian S Muntean Wissam eAbou-Alaiwi Andromeda eNauli Surya eNauli |
| author_facet | Kimberly Fisher Atkinson Sarmed eKathem Xingjian eJin Brian S Muntean Wissam eAbou-Alaiwi Andromeda eNauli Surya eNauli |
| author_sort | Kimberly Fisher Atkinson |
| collection | DOAJ |
| description | Activation of dopamine receptor type-5 (DR5) has been known to reduce systemic blood pressure, most likely by increasing renal vasodilation and enhancing natriuresis in the kidney. However, the mechanism of DR5 in natriuresis and vasodilation was not clearly known. We have previously shown that DR5 is localized to primary cilia of proximal renal epithelial and vascular endothelial cells. We here show that selective activation of DR5 specifically induces calcium influx only in the primary cilia, whereas non-selective activation of dopamine receptor induces calcium fluxes in both cilioplasm and cytoplasm. Cilia-independent signaling induced by thrombin only shows calcium signaling within cytoplasm. Furthermore, calcium activation in the cilioplasm by DR5 increases length and mechanosensory function of primary cilia, leading to a greater response to fluid-shear stress. We therefore propose a new mechanism by which DR5 induces vasodilation via chemical and mechanical properties that are specific to primary cilia. |
| first_indexed | 2024-12-16T18:11:14Z |
| format | Article |
| id | doaj.art-de5b3e760ced4512b6343a5b3437c0db |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-16T18:11:14Z |
| publishDate | 2015-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-de5b3e760ced4512b6343a5b3437c0db2022-12-21T22:21:47ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2015-04-01610.3389/fphys.2015.00103134747Dopaminergic signaling within the primary cilia in the renovascular systemKimberly Fisher Atkinson0Sarmed eKathem1Xingjian eJin2Brian S Muntean3Wissam eAbou-Alaiwi4Andromeda eNauli5Surya eNauli6Chapman UniversityChapman UniversityUniversity of ToledoUniversity of ToledoUniversity of ToledoCalifornia Northstate UniversityChapman UniversityActivation of dopamine receptor type-5 (DR5) has been known to reduce systemic blood pressure, most likely by increasing renal vasodilation and enhancing natriuresis in the kidney. However, the mechanism of DR5 in natriuresis and vasodilation was not clearly known. We have previously shown that DR5 is localized to primary cilia of proximal renal epithelial and vascular endothelial cells. We here show that selective activation of DR5 specifically induces calcium influx only in the primary cilia, whereas non-selective activation of dopamine receptor induces calcium fluxes in both cilioplasm and cytoplasm. Cilia-independent signaling induced by thrombin only shows calcium signaling within cytoplasm. Furthermore, calcium activation in the cilioplasm by DR5 increases length and mechanosensory function of primary cilia, leading to a greater response to fluid-shear stress. We therefore propose a new mechanism by which DR5 induces vasodilation via chemical and mechanical properties that are specific to primary cilia.http://journal.frontiersin.org/Journal/10.3389/fphys.2015.00103/fullBlood PressurePrimary Ciliumpolycystic kidney diseaseciliopathyCiliumciliotherapy |
| spellingShingle | Kimberly Fisher Atkinson Sarmed eKathem Xingjian eJin Brian S Muntean Wissam eAbou-Alaiwi Andromeda eNauli Surya eNauli Dopaminergic signaling within the primary cilia in the renovascular system Frontiers in Physiology Blood Pressure Primary Cilium polycystic kidney disease ciliopathy Cilium ciliotherapy |
| title | Dopaminergic signaling within the primary cilia in the renovascular system |
| title_full | Dopaminergic signaling within the primary cilia in the renovascular system |
| title_fullStr | Dopaminergic signaling within the primary cilia in the renovascular system |
| title_full_unstemmed | Dopaminergic signaling within the primary cilia in the renovascular system |
| title_short | Dopaminergic signaling within the primary cilia in the renovascular system |
| title_sort | dopaminergic signaling within the primary cilia in the renovascular system |
| topic | Blood Pressure Primary Cilium polycystic kidney disease ciliopathy Cilium ciliotherapy |
| url | http://journal.frontiersin.org/Journal/10.3389/fphys.2015.00103/full |
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