Single step biotransformation of corn oil phytosterols to boldenone by a newly isolated Pseudomonas aeruginosa
A new potent Pseudomonas aeruginosa isolate capable for biotransformation of corn oil phytosterol (PS) to 4-androstene-3, 17-dione (AD), testosterone (T) and boldenone (BOL) was identified by phenotypic analysis and 16S rRNA gene sequencing. Sequential statistical strategy was used to optimize the b...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016-09-01
|
| Series: | Biotechnology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X16300212 |
| _version_ | 1817981081124601856 |
|---|---|
| author | Mohamed Eisa Heba El-Refai Magdy Amin |
| author_facet | Mohamed Eisa Heba El-Refai Magdy Amin |
| author_sort | Mohamed Eisa |
| collection | DOAJ |
| description | A new potent Pseudomonas aeruginosa isolate capable for biotransformation of corn oil phytosterol (PS) to 4-androstene-3, 17-dione (AD), testosterone (T) and boldenone (BOL) was identified by phenotypic analysis and 16S rRNA gene sequencing. Sequential statistical strategy was used to optimize the biotransformation process mainly concerning BOL using Factorial design and response surface methodology (RSM). The production of BOL in single step microbial biotransformation from corn oil phytosterols by P. aeruginosa was not previously reported. Results showed that the pH concentration of the medium, (NH4)2SO4 and KH2PO4 were the most significant factors affecting BOL production. By analyzing the statistical model of three-dimensional surface plot, BOL production increased from 36.8% to 42.4% after the first step of optimization, and the overall biotransformation increased to 51.9%. After applying the second step of the sequential statistical strategy BOL production increased to 53.6%, and the overall biotransformation increased to 91.9% using the following optimized medium composition (g/l distilled water) (NH4)2SO4, 2; KH2PO4, 4; Na2HPO4. 1; MgSO4·7H2O, 0.3; NaCl, 0.1; CaCl2·2H2O, 0.1; FeSO4·7H2O, 0.001; ammonium acetate 0.001; Tween 80, 0.05%; corn oil 0.5%; 8-hydroxyquinoline 0.016; pH 8; 200 rpm agitation speed and incubation time 36 h at 30 °C. Validation experiments proved the adequacy and accuracy of model, and the results showed the predicted value agreed well with the experimental values. |
| first_indexed | 2024-04-13T23:01:15Z |
| format | Article |
| id | doaj.art-de5c55583a1248759bb34264e185e270 |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-04-13T23:01:15Z |
| publishDate | 2016-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-de5c55583a1248759bb34264e185e2702022-12-22T02:25:50ZengElsevierBiotechnology Reports2215-017X2016-09-0111C364310.1016/j.btre.2016.05.002Single step biotransformation of corn oil phytosterols to boldenone by a newly isolated Pseudomonas aeruginosaMohamed Eisa0Heba El-Refai1Magdy Amin2Department of Microbiology and Immunology, Faculty of Pharmacy, Al Azhar University, Assiut, EgyptChemistry of Natural and Microbial Products Department, National Research Center, Dokki, Giza, EgyptMicrobiology and Immunology Department, Faculty of Pharmacy, Cairo University, EgyptA new potent Pseudomonas aeruginosa isolate capable for biotransformation of corn oil phytosterol (PS) to 4-androstene-3, 17-dione (AD), testosterone (T) and boldenone (BOL) was identified by phenotypic analysis and 16S rRNA gene sequencing. Sequential statistical strategy was used to optimize the biotransformation process mainly concerning BOL using Factorial design and response surface methodology (RSM). The production of BOL in single step microbial biotransformation from corn oil phytosterols by P. aeruginosa was not previously reported. Results showed that the pH concentration of the medium, (NH4)2SO4 and KH2PO4 were the most significant factors affecting BOL production. By analyzing the statistical model of three-dimensional surface plot, BOL production increased from 36.8% to 42.4% after the first step of optimization, and the overall biotransformation increased to 51.9%. After applying the second step of the sequential statistical strategy BOL production increased to 53.6%, and the overall biotransformation increased to 91.9% using the following optimized medium composition (g/l distilled water) (NH4)2SO4, 2; KH2PO4, 4; Na2HPO4. 1; MgSO4·7H2O, 0.3; NaCl, 0.1; CaCl2·2H2O, 0.1; FeSO4·7H2O, 0.001; ammonium acetate 0.001; Tween 80, 0.05%; corn oil 0.5%; 8-hydroxyquinoline 0.016; pH 8; 200 rpm agitation speed and incubation time 36 h at 30 °C. Validation experiments proved the adequacy and accuracy of model, and the results showed the predicted value agreed well with the experimental values.http://www.sciencedirect.com/science/article/pii/S2215017X16300212AndrostenedioneBoldenoneCorn oil phytosterolsFactorial designPseudomonads aeruginosa |
| spellingShingle | Mohamed Eisa Heba El-Refai Magdy Amin Single step biotransformation of corn oil phytosterols to boldenone by a newly isolated Pseudomonas aeruginosa Biotechnology Reports Androstenedione Boldenone Corn oil phytosterols Factorial design Pseudomonads aeruginosa |
| title | Single step biotransformation of corn oil phytosterols to boldenone by a newly isolated Pseudomonas aeruginosa |
| title_full | Single step biotransformation of corn oil phytosterols to boldenone by a newly isolated Pseudomonas aeruginosa |
| title_fullStr | Single step biotransformation of corn oil phytosterols to boldenone by a newly isolated Pseudomonas aeruginosa |
| title_full_unstemmed | Single step biotransformation of corn oil phytosterols to boldenone by a newly isolated Pseudomonas aeruginosa |
| title_short | Single step biotransformation of corn oil phytosterols to boldenone by a newly isolated Pseudomonas aeruginosa |
| title_sort | single step biotransformation of corn oil phytosterols to boldenone by a newly isolated pseudomonas aeruginosa |
| topic | Androstenedione Boldenone Corn oil phytosterols Factorial design Pseudomonads aeruginosa |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X16300212 |
| work_keys_str_mv | AT mohamedeisa singlestepbiotransformationofcornoilphytosterolstoboldenonebyanewlyisolatedpseudomonasaeruginosa AT hebaelrefai singlestepbiotransformationofcornoilphytosterolstoboldenonebyanewlyisolatedpseudomonasaeruginosa AT magdyamin singlestepbiotransformationofcornoilphytosterolstoboldenonebyanewlyisolatedpseudomonasaeruginosa |