Lipid droplets characterization in adipocyte differentiated 3T3-L1 cells: size and optical density distribution
<p>The 3T3-L1 cell line, derived from 3T3 cells, is widely used in biological research on adipose tissue. 3T3-L1 cells have a fibroblast-like morphology, but, under appropriate conditions, they differentiate into an adipocyte-like phenotype. During the differentiation process, 3T3-L1 cells inc...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PAGEPress Publications
2013-08-01
|
| Series: | European Journal of Histochemistry |
| Subjects: | |
| Online Access: | http://www.ejh.it/index.php/ejh/article/view/2207 |
| _version_ | 1811285644560826368 |
|---|---|
| author | V. Rizzatti F. Boschi M. Pedrotti E. Zoico A. Sbarbati M. Zamboni |
| author_facet | V. Rizzatti F. Boschi M. Pedrotti E. Zoico A. Sbarbati M. Zamboni |
| author_sort | V. Rizzatti |
| collection | DOAJ |
| description | <p>The 3T3-L1 cell line, derived from 3T3 cells, is widely used in biological research on adipose tissue. 3T3-L1 cells have a fibroblast-like morphology, but, under appropriate conditions, they differentiate into an adipocyte-like phenotype. During the differentiation process, 3T3-L1 cells increase the synthesis of triglycerides and acquire the behavior of adipose cells. In particular, triglycerides accumulate in lipid droplets (LDs) embedded in the cytoplasm. The number and the size distribution of the LDs is often correlated with obesity and many other pathologies linked with fat accumulation. The integrated optical density (IOD) of the LDs is related with the amount of triglycerides in the droplets. The aim of this study is the attempt to characterize the size distribution and the IOD of the LDs in 3T3-L1 differentiated cells. The cells were differentiated into adipocytes for 5 days with a standard procedure, stained with Oil Red O and observed with an optical microscope. The diameter, area, optical density of the LDs were measured. We found an asymmetry of the kernel density distribution of the maximum Feret’s diameter of the LDs with a tail due to very large LDs. More information regarding the birth of the LDs could help in finding the best mathematical model in order to analyze fat accumulation in adipocytes.</p> |
| first_indexed | 2024-04-13T02:47:09Z |
| format | Article |
| id | doaj.art-de5d12da1a274aa49f11ed0aa120babe |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-04-13T02:47:09Z |
| publishDate | 2013-08-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-de5d12da1a274aa49f11ed0aa120babe2022-12-22T03:05:58ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062013-08-01573e24e2410.4081/ejh.2013.e241345Lipid droplets characterization in adipocyte differentiated 3T3-L1 cells: size and optical density distributionV. RizzattiF. BoschiM. PedrottiE. ZoicoA. SbarbatiM. Zamboni<p>The 3T3-L1 cell line, derived from 3T3 cells, is widely used in biological research on adipose tissue. 3T3-L1 cells have a fibroblast-like morphology, but, under appropriate conditions, they differentiate into an adipocyte-like phenotype. During the differentiation process, 3T3-L1 cells increase the synthesis of triglycerides and acquire the behavior of adipose cells. In particular, triglycerides accumulate in lipid droplets (LDs) embedded in the cytoplasm. The number and the size distribution of the LDs is often correlated with obesity and many other pathologies linked with fat accumulation. The integrated optical density (IOD) of the LDs is related with the amount of triglycerides in the droplets. The aim of this study is the attempt to characterize the size distribution and the IOD of the LDs in 3T3-L1 differentiated cells. The cells were differentiated into adipocytes for 5 days with a standard procedure, stained with Oil Red O and observed with an optical microscope. The diameter, area, optical density of the LDs were measured. We found an asymmetry of the kernel density distribution of the maximum Feret’s diameter of the LDs with a tail due to very large LDs. More information regarding the birth of the LDs could help in finding the best mathematical model in order to analyze fat accumulation in adipocytes.</p>http://www.ejh.it/index.php/ejh/article/view/2207Lipid droplet, 3T3-L1, adipocyte, fat, triglyceride accumulation, integrated optical density |
| spellingShingle | V. Rizzatti F. Boschi M. Pedrotti E. Zoico A. Sbarbati M. Zamboni Lipid droplets characterization in adipocyte differentiated 3T3-L1 cells: size and optical density distribution European Journal of Histochemistry Lipid droplet, 3T3-L1, adipocyte, fat, triglyceride accumulation, integrated optical density |
| title | Lipid droplets characterization in adipocyte differentiated 3T3-L1 cells: size and optical density distribution |
| title_full | Lipid droplets characterization in adipocyte differentiated 3T3-L1 cells: size and optical density distribution |
| title_fullStr | Lipid droplets characterization in adipocyte differentiated 3T3-L1 cells: size and optical density distribution |
| title_full_unstemmed | Lipid droplets characterization in adipocyte differentiated 3T3-L1 cells: size and optical density distribution |
| title_short | Lipid droplets characterization in adipocyte differentiated 3T3-L1 cells: size and optical density distribution |
| title_sort | lipid droplets characterization in adipocyte differentiated 3t3 l1 cells size and optical density distribution |
| topic | Lipid droplet, 3T3-L1, adipocyte, fat, triglyceride accumulation, integrated optical density |
| url | http://www.ejh.it/index.php/ejh/article/view/2207 |
| work_keys_str_mv | AT vrizzatti lipiddropletscharacterizationinadipocytedifferentiated3t3l1cellssizeandopticaldensitydistribution AT fboschi lipiddropletscharacterizationinadipocytedifferentiated3t3l1cellssizeandopticaldensitydistribution AT mpedrotti lipiddropletscharacterizationinadipocytedifferentiated3t3l1cellssizeandopticaldensitydistribution AT ezoico lipiddropletscharacterizationinadipocytedifferentiated3t3l1cellssizeandopticaldensitydistribution AT asbarbati lipiddropletscharacterizationinadipocytedifferentiated3t3l1cellssizeandopticaldensitydistribution AT mzamboni lipiddropletscharacterizationinadipocytedifferentiated3t3l1cellssizeandopticaldensitydistribution |