In vitro regeneration of double haploid line of African marigold (Tagetes erecta) derived from ovule culture using non-axillary explants
An efficient protocol has been developed for in vitro regeneration of double haploid line of African marigold variety Local Orange derived through ovule culture. Maximum survival (85%) of leaf explant was found in the treatment comprising of Bavistin (0.2%), Ridomil (0.2%) and 8-HQC (200 ppm). The s...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2019-06-01
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| Series: | The Indian Journal of Agricultural Sciences |
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| Online Access: | https://epubs.icar.org.in/index.php/IJAgS/article/view/90784 |
| _version_ | 1797896982817144832 |
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| author | UZMA MEHRAJ SAPNA PANWAR KANWAR PAL SINGH NAMITA Namita RENU PANDEY AMOLKUMAR U SOLANKE NIHARIKA MALLICK SURENDRA KUMAR |
| author_facet | UZMA MEHRAJ SAPNA PANWAR KANWAR PAL SINGH NAMITA Namita RENU PANDEY AMOLKUMAR U SOLANKE NIHARIKA MALLICK SURENDRA KUMAR |
| author_sort | UZMA MEHRAJ |
| collection | DOAJ |
| description | An efficient protocol has been developed for in vitro regeneration of double haploid line of African marigold variety Local Orange derived through ovule culture. Maximum survival (85%) of leaf explant was found in the treatment comprising of Bavistin (0.2%), Ridomil (0.2%) and 8-HQC (200 ppm). The surface sterilization treatment of explants with mercuric chloride (0.1%) for 3 minutes resulted in maximum survival (88.33%) and minimum contamination (18.33%). Three different portions of leaf explant (Tip, middle and basal) were used for regeneration. The treatment comprising MS medium supplemented with BAP (0.5 mg/l) and NAA (0.25 mg/l) resulted in maximum percent regeneration (86.25), lowest number of days taken for callus initiation (3.05), minimum number of days for shoot regeneration (13.25), maximum number of micro shoots per explant (3.50), minimum number of days to reach the stage of sub culturing (5.75) with basal portion of leaf explant. Among the different explants, basal portion of the leaf was found as the best explant for maximum regeneration. The basal portion of the leaf explant was further treated with best regeneration treatment, i. e. BAP (0.5 mg/l) and NAA (0.25 mg/l) along with different concentrations of putrescine and it was observed that maximum percent regeneration (89.83%), minimum number of days for shoot regeneration (13.50), maximum number of micro shoots per explant (6.25) and minimum number of days to reach the stage of subculturing (5.75) were found in the treatment combination of BAP (0.5 mg/l), NAA (0.25 mg/l) and Putrescine (50 mg/l). |
| first_indexed | 2024-04-10T07:51:28Z |
| format | Article |
| id | doaj.art-de6163a27e9e4155b81e55a7bba0290f |
| institution | Directory Open Access Journal |
| issn | 0019-5022 2394-3319 |
| language | English |
| last_indexed | 2024-04-10T07:51:28Z |
| publishDate | 2019-06-01 |
| publisher | Indian Council of Agricultural Research |
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| series | The Indian Journal of Agricultural Sciences |
| spelling | doaj.art-de6163a27e9e4155b81e55a7bba0290f2023-02-23T10:14:25ZengIndian Council of Agricultural ResearchThe Indian Journal of Agricultural Sciences0019-50222394-33192019-06-0189610.56093/ijas.v89i6.90784In vitro regeneration of double haploid line of African marigold (Tagetes erecta) derived from ovule culture using non-axillary explantsUZMA MEHRAJ0SAPNA PANWAR1KANWAR PAL SINGH2NAMITA Namita3RENU PANDEY4AMOLKUMAR U SOLANKE5NIHARIKA MALLICK6SURENDRA KUMAR7Research Scholar, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, IndiaScientist, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, IndiaPrincipal Scientist, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, IndiaScientist, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, IndiaScientist, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, IndiaScientist, National Research Centre on Plant Biotechnology, Pusa Campus, New Delhi.Scientist, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, IndiaAssistant Chief Technical Officer, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, IndiaAn efficient protocol has been developed for in vitro regeneration of double haploid line of African marigold variety Local Orange derived through ovule culture. Maximum survival (85%) of leaf explant was found in the treatment comprising of Bavistin (0.2%), Ridomil (0.2%) and 8-HQC (200 ppm). The surface sterilization treatment of explants with mercuric chloride (0.1%) for 3 minutes resulted in maximum survival (88.33%) and minimum contamination (18.33%). Three different portions of leaf explant (Tip, middle and basal) were used for regeneration. The treatment comprising MS medium supplemented with BAP (0.5 mg/l) and NAA (0.25 mg/l) resulted in maximum percent regeneration (86.25), lowest number of days taken for callus initiation (3.05), minimum number of days for shoot regeneration (13.25), maximum number of micro shoots per explant (3.50), minimum number of days to reach the stage of sub culturing (5.75) with basal portion of leaf explant. Among the different explants, basal portion of the leaf was found as the best explant for maximum regeneration. The basal portion of the leaf explant was further treated with best regeneration treatment, i. e. BAP (0.5 mg/l) and NAA (0.25 mg/l) along with different concentrations of putrescine and it was observed that maximum percent regeneration (89.83%), minimum number of days for shoot regeneration (13.50), maximum number of micro shoots per explant (6.25) and minimum number of days to reach the stage of subculturing (5.75) were found in the treatment combination of BAP (0.5 mg/l), NAA (0.25 mg/l) and Putrescine (50 mg/l).https://epubs.icar.org.in/index.php/IJAgS/article/view/90784Doubled haploid lineIn vitro regenerationNon axillary explantTagetes erecta L. |
| spellingShingle | UZMA MEHRAJ SAPNA PANWAR KANWAR PAL SINGH NAMITA Namita RENU PANDEY AMOLKUMAR U SOLANKE NIHARIKA MALLICK SURENDRA KUMAR In vitro regeneration of double haploid line of African marigold (Tagetes erecta) derived from ovule culture using non-axillary explants The Indian Journal of Agricultural Sciences Doubled haploid line In vitro regeneration Non axillary explant Tagetes erecta L. |
| title | In vitro regeneration of double haploid line of African marigold (Tagetes erecta) derived from ovule culture using non-axillary explants |
| title_full | In vitro regeneration of double haploid line of African marigold (Tagetes erecta) derived from ovule culture using non-axillary explants |
| title_fullStr | In vitro regeneration of double haploid line of African marigold (Tagetes erecta) derived from ovule culture using non-axillary explants |
| title_full_unstemmed | In vitro regeneration of double haploid line of African marigold (Tagetes erecta) derived from ovule culture using non-axillary explants |
| title_short | In vitro regeneration of double haploid line of African marigold (Tagetes erecta) derived from ovule culture using non-axillary explants |
| title_sort | in vitro regeneration of double haploid line of african marigold tagetes erecta derived from ovule culture using non axillary explants |
| topic | Doubled haploid line In vitro regeneration Non axillary explant Tagetes erecta L. |
| url | https://epubs.icar.org.in/index.php/IJAgS/article/view/90784 |
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