Shatavarin-IV saponin adjuvant elicits IgG and IgG2b responses against Staphylococcus aureus bacterin in a murine model
Asparagus adscendens Roxb. also known as “safed musli” or “shatavari” is a medicinal plant commonly found in South Asian countries. Shatavari is effective for the treatment of gastric ulcers, renal stones, bronchitis, diabetes, diabetic neuropathy, irritable bowel syndrome, alcohol withdrawal and ha...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S240584402302546X |
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| author | Arivukarasu Palanisamy Rinku Sharma Prithvi Pal Singh Upendra Sharma Rajendra Damu Patil Gorakh Mal Birbal Singh |
| author_facet | Arivukarasu Palanisamy Rinku Sharma Prithvi Pal Singh Upendra Sharma Rajendra Damu Patil Gorakh Mal Birbal Singh |
| author_sort | Arivukarasu Palanisamy |
| collection | DOAJ |
| description | Asparagus adscendens Roxb. also known as “safed musli” or “shatavari” is a medicinal plant commonly found in South Asian countries. Shatavari is effective for the treatment of gastric ulcers, renal stones, bronchitis, diabetes, diabetic neuropathy, irritable bowel syndrome, alcohol withdrawal and has reported immunostimulatory effects. In this study, the adjuvant potential of Shatavarin-IV saponin against Staphylococcus aureus bacterin in mice was investigated. Shatavarin-IV was evaluated for its toxicity and immunomodulatory potential against S. aureus bacterin in mice. Cellular and humoral immune responses were assessed. Shatavarin-IV was isolated from the fruit extract of Asparagus adscendens. The confirmation of the isolated molecule as Shatavarin-IV was done via TLC-based comparison with the standard molecule. Further, the structure was confirmed by using extensive spectroscopic analyses and comparing the observed data with literature reports. It was found safe up to the dose of 0.1 mg in the mice model. Shatavarin-IV adjuvant elicited IgG and IgG2b responses at the dose of 40 μg against S. aureus bacterin. However, the cell-mediated immune response was lesser as compared with the commercial Quil-A saponin . We demonstrated that Shatavarin-IV saponin adjuvant produced an optimum humoral immune response against S. aureus bacterin. These results highlight the potential of Shatavarin-IV as an adjuvant in a combination adjuvant in vaccine formulations for induction of potent immune response. |
| first_indexed | 2024-04-09T15:17:39Z |
| format | Article |
| id | doaj.art-de6433a48df04c36b8460c2ef9a23c19 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-09T15:17:39Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-de6433a48df04c36b8460c2ef9a23c192023-04-29T14:56:19ZengElsevierHeliyon2405-84402023-04-0194e15339Shatavarin-IV saponin adjuvant elicits IgG and IgG2b responses against Staphylococcus aureus bacterin in a murine modelArivukarasu Palanisamy0Rinku Sharma1Prithvi Pal Singh2Upendra Sharma3Rajendra Damu Patil4Gorakh Mal5Birbal Singh6Disease Investigation Laboratory, ICAR-Indian Veterinary Research Institute, Regional Station, Palampur 176061, Himachal Pradesh, IndiaDisease Investigation Laboratory, ICAR-Indian Veterinary Research Institute, Regional Station, Palampur 176061, Himachal Pradesh, India; Corresponding author. ICAR-Indian Veterinary Research Institute, Regional Station, Palampur 176 061, Himachal Pradesh, India.Chemical Technology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176 062, Himachal Pradesh, IndiaChemical Technology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176 062, Himachal Pradesh, IndiaDepartment of Veterinary Pathology, DGCN COVAS, CSK HPKV, Palampur 176 062, Himachal Pradesh, IndiaDisease Investigation Laboratory, ICAR-Indian Veterinary Research Institute, Regional Station, Palampur 176061, Himachal Pradesh, IndiaDisease Investigation Laboratory, ICAR-Indian Veterinary Research Institute, Regional Station, Palampur 176061, Himachal Pradesh, IndiaAsparagus adscendens Roxb. also known as “safed musli” or “shatavari” is a medicinal plant commonly found in South Asian countries. Shatavari is effective for the treatment of gastric ulcers, renal stones, bronchitis, diabetes, diabetic neuropathy, irritable bowel syndrome, alcohol withdrawal and has reported immunostimulatory effects. In this study, the adjuvant potential of Shatavarin-IV saponin against Staphylococcus aureus bacterin in mice was investigated. Shatavarin-IV was evaluated for its toxicity and immunomodulatory potential against S. aureus bacterin in mice. Cellular and humoral immune responses were assessed. Shatavarin-IV was isolated from the fruit extract of Asparagus adscendens. The confirmation of the isolated molecule as Shatavarin-IV was done via TLC-based comparison with the standard molecule. Further, the structure was confirmed by using extensive spectroscopic analyses and comparing the observed data with literature reports. It was found safe up to the dose of 0.1 mg in the mice model. Shatavarin-IV adjuvant elicited IgG and IgG2b responses at the dose of 40 μg against S. aureus bacterin. However, the cell-mediated immune response was lesser as compared with the commercial Quil-A saponin . We demonstrated that Shatavarin-IV saponin adjuvant produced an optimum humoral immune response against S. aureus bacterin. These results highlight the potential of Shatavarin-IV as an adjuvant in a combination adjuvant in vaccine formulations for induction of potent immune response.http://www.sciencedirect.com/science/article/pii/S240584402302546XShatavarin-IV (S-IV) isolation and characterizationAsparagus adscendens fruitInactivated S. aureus (Bacterin)Shatavarin-IV adjuvantImmune response |
| spellingShingle | Arivukarasu Palanisamy Rinku Sharma Prithvi Pal Singh Upendra Sharma Rajendra Damu Patil Gorakh Mal Birbal Singh Shatavarin-IV saponin adjuvant elicits IgG and IgG2b responses against Staphylococcus aureus bacterin in a murine model Heliyon Shatavarin-IV (S-IV) isolation and characterization Asparagus adscendens fruit Inactivated S. aureus (Bacterin) Shatavarin-IV adjuvant Immune response |
| title | Shatavarin-IV saponin adjuvant elicits IgG and IgG2b responses against Staphylococcus aureus bacterin in a murine model |
| title_full | Shatavarin-IV saponin adjuvant elicits IgG and IgG2b responses against Staphylococcus aureus bacterin in a murine model |
| title_fullStr | Shatavarin-IV saponin adjuvant elicits IgG and IgG2b responses against Staphylococcus aureus bacterin in a murine model |
| title_full_unstemmed | Shatavarin-IV saponin adjuvant elicits IgG and IgG2b responses against Staphylococcus aureus bacterin in a murine model |
| title_short | Shatavarin-IV saponin adjuvant elicits IgG and IgG2b responses against Staphylococcus aureus bacterin in a murine model |
| title_sort | shatavarin iv saponin adjuvant elicits igg and igg2b responses against staphylococcus aureus bacterin in a murine model |
| topic | Shatavarin-IV (S-IV) isolation and characterization Asparagus adscendens fruit Inactivated S. aureus (Bacterin) Shatavarin-IV adjuvant Immune response |
| url | http://www.sciencedirect.com/science/article/pii/S240584402302546X |
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