Apiospora arundinis, a panoply of carbohydrate-active enzymes and secondary metabolites
Abstract The Apiospora genus comprises filamentous fungi with promising potential, though its full capabilities remain undiscovered. In this study, we present the first genome assembly of an Apiospora arundinis isolate, demonstrating a highly complete and contiguous assembly estimated to 48.8 Mb, wi...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-04-01
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| Series: | IMA Fungus |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43008-024-00141-0 |
| _version_ | 1826844118555295744 |
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| author | Trine Sørensen Celine Petersen Asmus T. Muurmann Johan V. Christiansen Mathias L. Brundtø Christina K. Overgaard Anders T. Boysen Rasmus D. Wollenberg Thomas O. Larsen Jens L. Sørensen Kåre L. Nielsen Teis E. Sondergaard |
| author_facet | Trine Sørensen Celine Petersen Asmus T. Muurmann Johan V. Christiansen Mathias L. Brundtø Christina K. Overgaard Anders T. Boysen Rasmus D. Wollenberg Thomas O. Larsen Jens L. Sørensen Kåre L. Nielsen Teis E. Sondergaard |
| author_sort | Trine Sørensen |
| collection | DOAJ |
| description | Abstract The Apiospora genus comprises filamentous fungi with promising potential, though its full capabilities remain undiscovered. In this study, we present the first genome assembly of an Apiospora arundinis isolate, demonstrating a highly complete and contiguous assembly estimated to 48.8 Mb, with an N99 of 3.0 Mb. Our analysis predicted a total of 15,725 genes, with functional annotations for 13,619 of them, revealing a fungus capable of producing very high amounts of carbohydrate-active enzymes (CAZymes) and secondary metabolites. Through transcriptomic analysis, we observed differential gene expression in response to varying growth media, with several genes related to carbohydrate metabolism showing significant upregulation when the fungus was cultivated on a hay-based medium. Finally, our metabolomic analysis unveiled a fungus capable of producing a diverse array of metabolites. |
| first_indexed | 2024-04-24T12:37:47Z |
| format | Article |
| id | doaj.art-de65fb63a310423aba25ec2ed13824a8 |
| institution | Directory Open Access Journal |
| issn | 2210-6359 |
| language | English |
| last_indexed | 2025-02-16T13:57:58Z |
| publishDate | 2024-04-01 |
| publisher | BMC |
| record_format | Article |
| series | IMA Fungus |
| spelling | doaj.art-de65fb63a310423aba25ec2ed13824a82025-02-02T05:27:49ZengBMCIMA Fungus2210-63592024-04-0115111710.1186/s43008-024-00141-0Apiospora arundinis, a panoply of carbohydrate-active enzymes and secondary metabolitesTrine Sørensen0Celine Petersen1Asmus T. Muurmann2Johan V. Christiansen3Mathias L. Brundtø4Christina K. Overgaard5Anders T. Boysen6Rasmus D. Wollenberg7Thomas O. Larsen8Jens L. Sørensen9Kåre L. Nielsen10Teis E. Sondergaard11Department of Chemistry and Bioscience, Aalborg UniversityDepartment of Chemistry and Bioscience, Aalborg UniversityDepartment of Chemistry and Bioscience, Aalborg UniversityDepartment of Biotechnology and Biomedicine, Technical University of DenmarkDepartment of Chemistry and Bioscience, Aalborg UniversityDepartment of Chemistry and Bioscience, Aalborg UniversityDepartment of Chemistry and Bioscience, Aalborg UniversityDepartment of Chemistry and Bioscience, Aalborg UniversityDepartment of Biotechnology and Biomedicine, Technical University of DenmarkDepartment of Chemistry and Bioscience, Aalborg UniversityDepartment of Chemistry and Bioscience, Aalborg UniversityDepartment of Chemistry and Bioscience, Aalborg UniversityAbstract The Apiospora genus comprises filamentous fungi with promising potential, though its full capabilities remain undiscovered. In this study, we present the first genome assembly of an Apiospora arundinis isolate, demonstrating a highly complete and contiguous assembly estimated to 48.8 Mb, with an N99 of 3.0 Mb. Our analysis predicted a total of 15,725 genes, with functional annotations for 13,619 of them, revealing a fungus capable of producing very high amounts of carbohydrate-active enzymes (CAZymes) and secondary metabolites. Through transcriptomic analysis, we observed differential gene expression in response to varying growth media, with several genes related to carbohydrate metabolism showing significant upregulation when the fungus was cultivated on a hay-based medium. Finally, our metabolomic analysis unveiled a fungus capable of producing a diverse array of metabolites.https://doi.org/10.1186/s43008-024-00141-0Oxford Nanopore sequencingApiosporaArthriniumCAZymesSecondary metabolites |
| spellingShingle | Trine Sørensen Celine Petersen Asmus T. Muurmann Johan V. Christiansen Mathias L. Brundtø Christina K. Overgaard Anders T. Boysen Rasmus D. Wollenberg Thomas O. Larsen Jens L. Sørensen Kåre L. Nielsen Teis E. Sondergaard Apiospora arundinis, a panoply of carbohydrate-active enzymes and secondary metabolites IMA Fungus Oxford Nanopore sequencing Apiospora Arthrinium CAZymes Secondary metabolites |
| title | Apiospora arundinis, a panoply of carbohydrate-active enzymes and secondary metabolites |
| title_full | Apiospora arundinis, a panoply of carbohydrate-active enzymes and secondary metabolites |
| title_fullStr | Apiospora arundinis, a panoply of carbohydrate-active enzymes and secondary metabolites |
| title_full_unstemmed | Apiospora arundinis, a panoply of carbohydrate-active enzymes and secondary metabolites |
| title_short | Apiospora arundinis, a panoply of carbohydrate-active enzymes and secondary metabolites |
| title_sort | apiospora arundinis a panoply of carbohydrate active enzymes and secondary metabolites |
| topic | Oxford Nanopore sequencing Apiospora Arthrinium CAZymes Secondary metabolites |
| url | https://doi.org/10.1186/s43008-024-00141-0 |
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