Effect of grape seed extract on the oxidative and proliferative status of porcine intestinal epithelial cells
The aim of this study was to investigate the oxidative and proliferative effects of grape seed extract (GSE). Piglet intestinal epithelial cells (IPEC1) were selected as an unstressed cell model, or they were exposed to 400 μM H2O2 to establish a H2O2-stimulated cell model. The glutathione (GSH) and...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2018-05-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=2369 |
| _version_ | 1797800672344670208 |
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| author | Jinhe KANG Tan YANG Zhaoliang WU Jing YANG Min XIONG Zhiliang TAN Zhihong SUN Wenjun XIAO |
| author_facet | Jinhe KANG Tan YANG Zhaoliang WU Jing YANG Min XIONG Zhiliang TAN Zhihong SUN Wenjun XIAO |
| author_sort | Jinhe KANG |
| collection | DOAJ |
| description | The aim of this study was to investigate the oxidative and proliferative effects of grape seed extract (GSE). Piglet intestinal epithelial cells (IPEC1)
were selected as an unstressed cell model, or they were exposed to 400 μM H2O2 to establish a H2O2-stimulated cell model. The glutathione
(GSH) and total antioxidant capacity in response to GSE addition were tested in the unstressed and H2O2-stimulated cell models. The relative
mRNA levels of antioxidant or antioxidant enzymes and apoptosis-related genes were measured by Real-Time RT-PCR. In the unstressed status,
the cell survival ratio and GSH increased with the addition of GSE at 1 and 10 μg/mL but diminished at 60 μg GSE/mL. The addition of 1 μg/mL
GSE upregulated the mRNA expression levels of B-Cell Lymphoma protein-2 (Bcl-2), cysteine aspartases-3 (Caspase-3), cysteine aspartases-8
(Caspase-8) and glutathione peroxidase-1(GPx-1), while it downregulated that of Bcl2-associated X protein (Bax), copper-zinc superoxide
dismutase, glutathione S-transferase (GST), thioredoxin, thioltransferase and thioredoxin reductase. As GSE reached 60 μg/mL, the tumor protein
p53 (p53) and caspase-8 gene expressions were upregulated. In stressed status, 1 and 10 μg GSE/mL promoted the increase of GSH. H2O2-
induced increases in Bax, p53, and Caspase-3 mRNA expressions were attenuated by the subsequent addition of 1 μg GSE/mL and promoted the
gene expression of tumor necrosis factor-α, GPx-1 and thioltransferase (Ttas). Treatment with 60 μg GSE/ml resulted in a significant reduction in
Bax, p53, manganese superoxide dismutase and GST mRNA expressions. These results indicate that GSE exhibits antioxidative and proliferative
functions on unstressed IPEC1 cells at low and medium levels and oxidative and antiproliferative functions at high levels. |
| first_indexed | 2024-03-13T04:37:45Z |
| format | Article |
| id | doaj.art-de697f954b1e4bb2b20ca749088e9e0a |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:37:45Z |
| publishDate | 2018-05-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-de697f954b1e4bb2b20ca749088e9e0a2023-06-19T06:41:24ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512018-05-0124451151710.9775/kvfd.2018.194572369Effect of grape seed extract on the oxidative and proliferative status of porcine intestinal epithelial cellsJinhe KANG0Tan YANG1Zhaoliang WU2Jing YANG3Min XIONG4Zhiliang TAN5Zhihong SUN6Wenjun XIAO7Key Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Research Center of Livestock & Poultry, Sciences, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, P. R. CHINAKey Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Research Center of Livestock & Poultry, Sciences, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, P. R. CHINAKey Laboratory for Bio-feed and Molecular Nutrition, Southwest University, Chongqing 400715, P. R. CHINAKey Laboratory for Bio-feed and Molecular Nutrition, Southwest University, Chongqing 400715, P. R. CHINAKey Laboratory for Bio-feed and Molecular Nutrition, Southwest University, Chongqing 400715, P. R. CHINAKey Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Research Center of Livestock & Poultry, Sciences, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, P. R. CHINAKey Laboratory for Bio-feed and Molecular Nutrition, Southwest University, Chongqing 400715, P. R. CHINANational Research Center of Engineering & Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, Hunan 410128, P. R. CHINAThe aim of this study was to investigate the oxidative and proliferative effects of grape seed extract (GSE). Piglet intestinal epithelial cells (IPEC1) were selected as an unstressed cell model, or they were exposed to 400 μM H2O2 to establish a H2O2-stimulated cell model. The glutathione (GSH) and total antioxidant capacity in response to GSE addition were tested in the unstressed and H2O2-stimulated cell models. The relative mRNA levels of antioxidant or antioxidant enzymes and apoptosis-related genes were measured by Real-Time RT-PCR. In the unstressed status, the cell survival ratio and GSH increased with the addition of GSE at 1 and 10 μg/mL but diminished at 60 μg GSE/mL. The addition of 1 μg/mL GSE upregulated the mRNA expression levels of B-Cell Lymphoma protein-2 (Bcl-2), cysteine aspartases-3 (Caspase-3), cysteine aspartases-8 (Caspase-8) and glutathione peroxidase-1(GPx-1), while it downregulated that of Bcl2-associated X protein (Bax), copper-zinc superoxide dismutase, glutathione S-transferase (GST), thioredoxin, thioltransferase and thioredoxin reductase. As GSE reached 60 μg/mL, the tumor protein p53 (p53) and caspase-8 gene expressions were upregulated. In stressed status, 1 and 10 μg GSE/mL promoted the increase of GSH. H2O2- induced increases in Bax, p53, and Caspase-3 mRNA expressions were attenuated by the subsequent addition of 1 μg GSE/mL and promoted the gene expression of tumor necrosis factor-α, GPx-1 and thioltransferase (Ttas). Treatment with 60 μg GSE/ml resulted in a significant reduction in Bax, p53, manganese superoxide dismutase and GST mRNA expressions. These results indicate that GSE exhibits antioxidative and proliferative functions on unstressed IPEC1 cells at low and medium levels and oxidative and antiproliferative functions at high levels.https://vetdergikafkas.org/pdf.php?id=2369grape seed extractantioxidationproliferationintestinal epithelial cells |
| spellingShingle | Jinhe KANG Tan YANG Zhaoliang WU Jing YANG Min XIONG Zhiliang TAN Zhihong SUN Wenjun XIAO Effect of grape seed extract on the oxidative and proliferative status of porcine intestinal epithelial cells Kafkas Universitesi Veteriner Fakültesi Dergisi grape seed extract antioxidation proliferation intestinal epithelial cells |
| title | Effect of grape seed extract on the oxidative and proliferative status of porcine intestinal epithelial cells |
| title_full | Effect of grape seed extract on the oxidative and proliferative status of porcine intestinal epithelial cells |
| title_fullStr | Effect of grape seed extract on the oxidative and proliferative status of porcine intestinal epithelial cells |
| title_full_unstemmed | Effect of grape seed extract on the oxidative and proliferative status of porcine intestinal epithelial cells |
| title_short | Effect of grape seed extract on the oxidative and proliferative status of porcine intestinal epithelial cells |
| title_sort | effect of grape seed extract on the oxidative and proliferative status of porcine intestinal epithelial cells |
| topic | grape seed extract antioxidation proliferation intestinal epithelial cells |
| url | https://vetdergikafkas.org/pdf.php?id=2369 |
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