Diversity of Mycobacterium tuberculosis lineages in French Polynesia
Background/Purpose: French Polynesia is an overseas territory located in the South Pacific. The incidence of tuberculosis in French Polynesia has been stable since 2000 with an average of 20 cases/y/100,000 inhabitants. Molecular epidemiology of Mycobacterium tuberculosis in French Polynesia is unkn...
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| Format: | Article |
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Elsevier
2017-04-01
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| Series: | Journal of Microbiology, Immunology and Infection |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1684118215007744 |
| _version_ | 1819231872413073408 |
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| author | Djaltou Aboubaker Osman Michael Phelippeau Michel Drancourt Didier Musso |
| author_facet | Djaltou Aboubaker Osman Michael Phelippeau Michel Drancourt Didier Musso |
| author_sort | Djaltou Aboubaker Osman |
| collection | DOAJ |
| description | Background/Purpose: French Polynesia is an overseas territory located in the South Pacific. The incidence of tuberculosis in French Polynesia has been stable since 2000 with an average of 20 cases/y/100,000 inhabitants. Molecular epidemiology of Mycobacterium tuberculosis in French Polynesia is unknown because M. tuberculosis isolates have not been routinely genotyped.
Methods: From 2009 to 2012, 34 isolates collected from 32 French Polynesian patients were identified as M. tuberculosis by probe hybridization. These isolates were genotyped using spoligotyping and 24-loci mycobacterial interspersed repetitive units (MIRUs)-variable number of tandem repeat (VNTR). Spoligotype patterns obtained using commercial kits were compared with the online international database SITVIT. MIRU-VNTR genotyping was performed using an in-house protocol based on capillary electrophoresis sizing for 24-loci MIRU-VNTR genotyping.
Results: The results of the spoligotyping method revealed that 25 isolates grouped into six previously described spoligotypes [H1, H3, U likely (S), T1, Manu, and Beijing] and nine isolates grouped into six new spoligotypes. Comparison with the international database MIRU-VNTRplus distributed 30 isolates into five lineages (Haarlem, Latin American Mediterranean, S, X, and Beijing) and four as unassigned isolates.
Conclusion: Genotyping identified four phylogenetic lineages belonging to the modern Euro–American subgroup, one Beijing genotype responsible for worldwide pandemics, including remote islands in the South Pacific, and one Manu genotype of the ancestral lineage of M. tuberculosis. |
| first_indexed | 2024-12-23T11:51:52Z |
| format | Article |
| id | doaj.art-de6b08386fa849ba9b3928927c57c8f8 |
| institution | Directory Open Access Journal |
| issn | 1684-1182 |
| language | English |
| last_indexed | 2024-12-23T11:51:52Z |
| publishDate | 2017-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Microbiology, Immunology and Infection |
| spelling | doaj.art-de6b08386fa849ba9b3928927c57c8f82022-12-21T17:48:11ZengElsevierJournal of Microbiology, Immunology and Infection1684-11822017-04-0150219920610.1016/j.jmii.2015.05.018Diversity of Mycobacterium tuberculosis lineages in French PolynesiaDjaltou Aboubaker Osman0Michael Phelippeau1Michel Drancourt2Didier Musso3Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UMR CNRS 7278, IRD 198, INSERM 1095, Faculté de Médecine, Marseille, FranceUnité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UMR CNRS 7278, IRD 198, INSERM 1095, Faculté de Médecine, Marseille, FranceUnité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UMR CNRS 7278, IRD 198, INSERM 1095, Faculté de Médecine, Marseille, FrancePôle de recherche et de veille sur les maladies infectieuses émergente, Institut Louis Malardé, Tahiti, French PolynesiaBackground/Purpose: French Polynesia is an overseas territory located in the South Pacific. The incidence of tuberculosis in French Polynesia has been stable since 2000 with an average of 20 cases/y/100,000 inhabitants. Molecular epidemiology of Mycobacterium tuberculosis in French Polynesia is unknown because M. tuberculosis isolates have not been routinely genotyped. Methods: From 2009 to 2012, 34 isolates collected from 32 French Polynesian patients were identified as M. tuberculosis by probe hybridization. These isolates were genotyped using spoligotyping and 24-loci mycobacterial interspersed repetitive units (MIRUs)-variable number of tandem repeat (VNTR). Spoligotype patterns obtained using commercial kits were compared with the online international database SITVIT. MIRU-VNTR genotyping was performed using an in-house protocol based on capillary electrophoresis sizing for 24-loci MIRU-VNTR genotyping. Results: The results of the spoligotyping method revealed that 25 isolates grouped into six previously described spoligotypes [H1, H3, U likely (S), T1, Manu, and Beijing] and nine isolates grouped into six new spoligotypes. Comparison with the international database MIRU-VNTRplus distributed 30 isolates into five lineages (Haarlem, Latin American Mediterranean, S, X, and Beijing) and four as unassigned isolates. Conclusion: Genotyping identified four phylogenetic lineages belonging to the modern Euro–American subgroup, one Beijing genotype responsible for worldwide pandemics, including remote islands in the South Pacific, and one Manu genotype of the ancestral lineage of M. tuberculosis.http://www.sciencedirect.com/science/article/pii/S1684118215007744French PolynesiaGenotypingMycobacterial interspersed repetitive unitMycobacterium tuberculosisSpoligotypingtuberculosis |
| spellingShingle | Djaltou Aboubaker Osman Michael Phelippeau Michel Drancourt Didier Musso Diversity of Mycobacterium tuberculosis lineages in French Polynesia Journal of Microbiology, Immunology and Infection French Polynesia Genotyping Mycobacterial interspersed repetitive unit Mycobacterium tuberculosis Spoligotyping tuberculosis |
| title | Diversity of Mycobacterium tuberculosis lineages in French Polynesia |
| title_full | Diversity of Mycobacterium tuberculosis lineages in French Polynesia |
| title_fullStr | Diversity of Mycobacterium tuberculosis lineages in French Polynesia |
| title_full_unstemmed | Diversity of Mycobacterium tuberculosis lineages in French Polynesia |
| title_short | Diversity of Mycobacterium tuberculosis lineages in French Polynesia |
| title_sort | diversity of mycobacterium tuberculosis lineages in french polynesia |
| topic | French Polynesia Genotyping Mycobacterial interspersed repetitive unit Mycobacterium tuberculosis Spoligotyping tuberculosis |
| url | http://www.sciencedirect.com/science/article/pii/S1684118215007744 |
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