A Cas9 with PAM recognition for adenine dinucleotides
Protospacer adjacent motif (PAM) requirements limit the target range of CRISPR endonucleases. Here, the authors graft the 5$$^{\prime}$$ ′ -NAAN-3$$^{\prime}$$ ′ PAM-interacting domain of SmacCas9 onto SpyCas9 to create adenine dinucleotide targeting chimeras.
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2020-05-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-16117-8 |
| _version_ | 1818344666992476160 |
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| author | Pranam Chatterjee Jooyoung Lee Lisa Nip Sabrina R. T. Koseki Emma Tysinger Erik J. Sontheimer Joseph M. Jacobson Noah Jakimo |
| author_facet | Pranam Chatterjee Jooyoung Lee Lisa Nip Sabrina R. T. Koseki Emma Tysinger Erik J. Sontheimer Joseph M. Jacobson Noah Jakimo |
| author_sort | Pranam Chatterjee |
| collection | DOAJ |
| description | Protospacer adjacent motif (PAM) requirements limit the target range of CRISPR endonucleases. Here, the authors graft the 5$$^{\prime}$$ ′ -NAAN-3$$^{\prime}$$ ′ PAM-interacting domain of SmacCas9 onto SpyCas9 to create adenine dinucleotide targeting chimeras. |
| first_indexed | 2024-12-13T16:50:07Z |
| format | Article |
| id | doaj.art-de6c445336ca4aacb34b61cb8daeb358 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-13T16:50:07Z |
| publishDate | 2020-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-de6c445336ca4aacb34b61cb8daeb3582022-12-21T23:38:04ZengNature PortfolioNature Communications2041-17232020-05-011111610.1038/s41467-020-16117-8A Cas9 with PAM recognition for adenine dinucleotidesPranam Chatterjee0Jooyoung Lee1Lisa Nip2Sabrina R. T. Koseki3Emma Tysinger4Erik J. Sontheimer5Joseph M. Jacobson6Noah Jakimo7Center for Bits and AtomsRNA Therapeutics Institute, University of Massachusetts Medical SchoolCenter for Bits and AtomsCenter for Bits and AtomsCenter for Bits and AtomsRNA Therapeutics Institute, University of Massachusetts Medical SchoolCenter for Bits and AtomsCenter for Bits and AtomsProtospacer adjacent motif (PAM) requirements limit the target range of CRISPR endonucleases. Here, the authors graft the 5$$^{\prime}$$ ′ -NAAN-3$$^{\prime}$$ ′ PAM-interacting domain of SmacCas9 onto SpyCas9 to create adenine dinucleotide targeting chimeras.https://doi.org/10.1038/s41467-020-16117-8 |
| spellingShingle | Pranam Chatterjee Jooyoung Lee Lisa Nip Sabrina R. T. Koseki Emma Tysinger Erik J. Sontheimer Joseph M. Jacobson Noah Jakimo A Cas9 with PAM recognition for adenine dinucleotides Nature Communications |
| title | A Cas9 with PAM recognition for adenine dinucleotides |
| title_full | A Cas9 with PAM recognition for adenine dinucleotides |
| title_fullStr | A Cas9 with PAM recognition for adenine dinucleotides |
| title_full_unstemmed | A Cas9 with PAM recognition for adenine dinucleotides |
| title_short | A Cas9 with PAM recognition for adenine dinucleotides |
| title_sort | cas9 with pam recognition for adenine dinucleotides |
| url | https://doi.org/10.1038/s41467-020-16117-8 |
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