Development and Validation of an m6A RNA Methylation Regulator-Based Signature for Prognostic Prediction in Cervical Squamous Cell Carcinoma
Background: Cervical squamous cell carcinoma (CESC) is one of the most common causes of cancer-related death worldwide. N6-methyladenosine (m6A) plays an important role in various cellular responses by regulating mRNA biology. This study aimed to develop and validate an m6A RNA methylation regulator...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-08-01
|
| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fonc.2020.01444/full |
| _version_ | 1818204723064340480 |
|---|---|
| author | Jingxin Pan Lichao Xu Lichao Xu Hongda Pan |
| author_facet | Jingxin Pan Lichao Xu Lichao Xu Hongda Pan |
| author_sort | Jingxin Pan |
| collection | DOAJ |
| description | Background: Cervical squamous cell carcinoma (CESC) is one of the most common causes of cancer-related death worldwide. N6-methyladenosine (m6A) plays an important role in various cellular responses by regulating mRNA biology. This study aimed to develop and validate an m6A RNA methylation regulator-based signature for prognostic prediction in CESC.Methods: Clinical and survival data as well as RNA sequencing data of 13 m6A RNA methylation regulators were obtained from The Cancer Genome Atlas (TCGA) CESC database. Consensus clustering was performed to identify different CESC clusters based on the differential expression of the regulators. LASSO Cox regression analysis was used to generate a prognostic signature based on m6A RNA methylation regulator expression. The effect of the signature was further explored by univariate and multivariate Cox analyses.Results: Four regulators (RBM15, METTL3, FTO, and YTHDF2) were identified to be aberrantly expressed in CESC tissues. A prognostic signature that includes ZC3H13, YTHDC1, and YTHDF1 was developed, which can act as an independent prognostic indicator. Significant differences of survival rate and clinicopathological features were found between the high- and low-risk groups. The results of bioinformatics analysis were then validated in the clinical CESC cohort by qRT-PCR and immunohistochemistry staining.Conclusion: In the present study, we developed and validated an m6A RNA methylation regulator-based prognostic signature, which might provide useful insights regarding the development and prognosis of CESC. |
| first_indexed | 2024-12-12T03:45:46Z |
| format | Article |
| id | doaj.art-de6fb7b4598a4fd190ccc5db474797ce |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-12T03:45:46Z |
| publishDate | 2020-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-de6fb7b4598a4fd190ccc5db474797ce2022-12-22T00:39:34ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2020-08-011010.3389/fonc.2020.01444544982Development and Validation of an m6A RNA Methylation Regulator-Based Signature for Prognostic Prediction in Cervical Squamous Cell CarcinomaJingxin Pan0Lichao Xu1Lichao Xu2Hongda Pan3Department of Internal Medicine, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, ChinaDepartment of Interventional Radiology, Fudan University Shanghai Cancer Center, Shanghai, ChinaDepartment of Oncology, Shanghai Medical College, Fudan University, Shanghai, ChinaDepartment of Oncology, Shanghai Medical College, Fudan University, Shanghai, ChinaBackground: Cervical squamous cell carcinoma (CESC) is one of the most common causes of cancer-related death worldwide. N6-methyladenosine (m6A) plays an important role in various cellular responses by regulating mRNA biology. This study aimed to develop and validate an m6A RNA methylation regulator-based signature for prognostic prediction in CESC.Methods: Clinical and survival data as well as RNA sequencing data of 13 m6A RNA methylation regulators were obtained from The Cancer Genome Atlas (TCGA) CESC database. Consensus clustering was performed to identify different CESC clusters based on the differential expression of the regulators. LASSO Cox regression analysis was used to generate a prognostic signature based on m6A RNA methylation regulator expression. The effect of the signature was further explored by univariate and multivariate Cox analyses.Results: Four regulators (RBM15, METTL3, FTO, and YTHDF2) were identified to be aberrantly expressed in CESC tissues. A prognostic signature that includes ZC3H13, YTHDC1, and YTHDF1 was developed, which can act as an independent prognostic indicator. Significant differences of survival rate and clinicopathological features were found between the high- and low-risk groups. The results of bioinformatics analysis were then validated in the clinical CESC cohort by qRT-PCR and immunohistochemistry staining.Conclusion: In the present study, we developed and validated an m6A RNA methylation regulator-based prognostic signature, which might provide useful insights regarding the development and prognosis of CESC.https://www.frontiersin.org/article/10.3389/fonc.2020.01444/fullm6ARNA methylationcervical squamous cell carcinomaprognosisexperimental validation |
| spellingShingle | Jingxin Pan Lichao Xu Lichao Xu Hongda Pan Development and Validation of an m6A RNA Methylation Regulator-Based Signature for Prognostic Prediction in Cervical Squamous Cell Carcinoma Frontiers in Oncology m6A RNA methylation cervical squamous cell carcinoma prognosis experimental validation |
| title | Development and Validation of an m6A RNA Methylation Regulator-Based Signature for Prognostic Prediction in Cervical Squamous Cell Carcinoma |
| title_full | Development and Validation of an m6A RNA Methylation Regulator-Based Signature for Prognostic Prediction in Cervical Squamous Cell Carcinoma |
| title_fullStr | Development and Validation of an m6A RNA Methylation Regulator-Based Signature for Prognostic Prediction in Cervical Squamous Cell Carcinoma |
| title_full_unstemmed | Development and Validation of an m6A RNA Methylation Regulator-Based Signature for Prognostic Prediction in Cervical Squamous Cell Carcinoma |
| title_short | Development and Validation of an m6A RNA Methylation Regulator-Based Signature for Prognostic Prediction in Cervical Squamous Cell Carcinoma |
| title_sort | development and validation of an m6a rna methylation regulator based signature for prognostic prediction in cervical squamous cell carcinoma |
| topic | m6A RNA methylation cervical squamous cell carcinoma prognosis experimental validation |
| url | https://www.frontiersin.org/article/10.3389/fonc.2020.01444/full |
| work_keys_str_mv | AT jingxinpan developmentandvalidationofanm6arnamethylationregulatorbasedsignatureforprognosticpredictionincervicalsquamouscellcarcinoma AT lichaoxu developmentandvalidationofanm6arnamethylationregulatorbasedsignatureforprognosticpredictionincervicalsquamouscellcarcinoma AT lichaoxu developmentandvalidationofanm6arnamethylationregulatorbasedsignatureforprognosticpredictionincervicalsquamouscellcarcinoma AT hongdapan developmentandvalidationofanm6arnamethylationregulatorbasedsignatureforprognosticpredictionincervicalsquamouscellcarcinoma |