Phytochemical Screening by LC-ESI-MS/MS and Effect of the Ethyl Acetate Fraction from Leaves and Stems of <i>Jatropha macrantha</i> Müll Arg. on Ketamine-Induced Erectile Dysfunction in Rats
<i>Jatropha macrantha</i> Müll Arg. L is also known as “huanarpo macho” and used in the Peruvian traditional medicine as an aphrodisiac and erectile dysfunction (ED). The aim of this study was to determine the phytochemical constituents in leaves and stems ethyl acetate fraction (LEAF an...
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2021-12-01
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| author | Johnny Aldo Tinco-Jayo Enrique Javier Aguilar-Felices Edwin Carlos Enciso-Roca Jorge Luis Arroyo-Acevedo Oscar Herrera-Calderon |
| author_facet | Johnny Aldo Tinco-Jayo Enrique Javier Aguilar-Felices Edwin Carlos Enciso-Roca Jorge Luis Arroyo-Acevedo Oscar Herrera-Calderon |
| author_sort | Johnny Aldo Tinco-Jayo |
| collection | DOAJ |
| description | <i>Jatropha macrantha</i> Müll Arg. L is also known as “huanarpo macho” and used in the Peruvian traditional medicine as an aphrodisiac and erectile dysfunction (ED). The aim of this study was to determine the phytochemical constituents in leaves and stems ethyl acetate fraction (LEAF and SEAF) of <i>J. macrantha</i> and to compare the antioxidant activity and the ameliorative effect on ketamine-induced erectile dysfunction in rats. The phytochemical constituents were determined by LC-ESI-MS/MS, the total phenolic compounds and total flavonoids (TPC and TF) by Folin-Ciocalteu and aluminum chloride, respectively. The antioxidant activity was determined by DPPH, ABTS, and FRAP assays. Experimental groups were divided as follows: I: negative control; II: positive control (ketamine at 50 mg/ kg/d); III: sildenafil 5 mg/kg; IV, V, VI: LEAF at 25, 50 and 100 mg/kg, respectively, and VII, VIII, IX: SEAF at 25, 50, and 100 mg/kg, respectively. The phytochemical analysis revealed the presence mainly of coumarins, flavonoids, phenolic acids, and terpenes. TPC of LEAF and SEAF were 359 ± 5.21 mg GAE/g and 306 ± 1.93 mg GAE/g, respectively; TF in LEAF and SEAF were 23.7 ± 0.80 mg EQ/g, and 101 ± 1.42 mg EQ/g, respectively. The DPPH, ABTS, FRAP in SEAF were 647 ± 3.27; 668 ± 2.30; and 575 ± 2.86 μmol TE/g, respectively, whilst LEAF showed 796 ± 3.15; 679 ± 0.85; and 806 ± 3.42 μmol TE/g, respectively. Regarding sexual behavior, LEAF showed a better effect in mount frequency, intromission frequency, ejaculation frequency, mount latency, intromission latency, ejaculatory latency, and post ejaculatory latency than SEAF. As conclusion, LEAF of <i>J. macrantha</i> at 50 mg/kg showed a better effect on sexual behavior in male rats with erectile dysfunction than SEAF but not higher than sildenafil. |
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| spelling | doaj.art-de721dddb15d448fa133c83eed6aae8a2023-11-23T11:56:56ZengMDPI AGMolecules1420-30492021-12-0127111510.3390/molecules27010115Phytochemical Screening by LC-ESI-MS/MS and Effect of the Ethyl Acetate Fraction from Leaves and Stems of <i>Jatropha macrantha</i> Müll Arg. on Ketamine-Induced Erectile Dysfunction in RatsJohnny Aldo Tinco-Jayo0Enrique Javier Aguilar-Felices1Edwin Carlos Enciso-Roca2Jorge Luis Arroyo-Acevedo3Oscar Herrera-Calderon4Department of Human Medicine, Faculty of Health Sciences, Universidad Nacional de San Cristobal de Huamanga, Portal Independencia 57, Ayacucho 05003, PeruDepartment of Human Medicine, Faculty of Health Sciences, Universidad Nacional de San Cristobal de Huamanga, Portal Independencia 57, Ayacucho 05003, PeruDepartment of Human Medicine, Faculty of Health Sciences, Universidad Nacional de San Cristobal de Huamanga, Portal Independencia 57, Ayacucho 05003, PeruDepartment of Dynamic Sciences, Faculty of Medicine, Universidad Nacional Mayor de San Marcos, Av. Miguel Grau 755, Lima 15001, PeruDepartment of Pharmacology, Bromatology and Toxicology, Faculty of Pharmacy and Biochemistry, Universidad Nacional Mayor de San Marcos, Jr. Puno 1002, Lima 15001, Peru<i>Jatropha macrantha</i> Müll Arg. L is also known as “huanarpo macho” and used in the Peruvian traditional medicine as an aphrodisiac and erectile dysfunction (ED). The aim of this study was to determine the phytochemical constituents in leaves and stems ethyl acetate fraction (LEAF and SEAF) of <i>J. macrantha</i> and to compare the antioxidant activity and the ameliorative effect on ketamine-induced erectile dysfunction in rats. The phytochemical constituents were determined by LC-ESI-MS/MS, the total phenolic compounds and total flavonoids (TPC and TF) by Folin-Ciocalteu and aluminum chloride, respectively. The antioxidant activity was determined by DPPH, ABTS, and FRAP assays. Experimental groups were divided as follows: I: negative control; II: positive control (ketamine at 50 mg/ kg/d); III: sildenafil 5 mg/kg; IV, V, VI: LEAF at 25, 50 and 100 mg/kg, respectively, and VII, VIII, IX: SEAF at 25, 50, and 100 mg/kg, respectively. The phytochemical analysis revealed the presence mainly of coumarins, flavonoids, phenolic acids, and terpenes. TPC of LEAF and SEAF were 359 ± 5.21 mg GAE/g and 306 ± 1.93 mg GAE/g, respectively; TF in LEAF and SEAF were 23.7 ± 0.80 mg EQ/g, and 101 ± 1.42 mg EQ/g, respectively. The DPPH, ABTS, FRAP in SEAF were 647 ± 3.27; 668 ± 2.30; and 575 ± 2.86 μmol TE/g, respectively, whilst LEAF showed 796 ± 3.15; 679 ± 0.85; and 806 ± 3.42 μmol TE/g, respectively. Regarding sexual behavior, LEAF showed a better effect in mount frequency, intromission frequency, ejaculation frequency, mount latency, intromission latency, ejaculatory latency, and post ejaculatory latency than SEAF. As conclusion, LEAF of <i>J. macrantha</i> at 50 mg/kg showed a better effect on sexual behavior in male rats with erectile dysfunction than SEAF but not higher than sildenafil.https://www.mdpi.com/1420-3049/27/1/115antioxidant agentaphrodisiacliquid chromatographyhuanarposexual behaviorexperimental model |
| spellingShingle | Johnny Aldo Tinco-Jayo Enrique Javier Aguilar-Felices Edwin Carlos Enciso-Roca Jorge Luis Arroyo-Acevedo Oscar Herrera-Calderon Phytochemical Screening by LC-ESI-MS/MS and Effect of the Ethyl Acetate Fraction from Leaves and Stems of <i>Jatropha macrantha</i> Müll Arg. on Ketamine-Induced Erectile Dysfunction in Rats Molecules antioxidant agent aphrodisiac liquid chromatography huanarpo sexual behavior experimental model |
| title | Phytochemical Screening by LC-ESI-MS/MS and Effect of the Ethyl Acetate Fraction from Leaves and Stems of <i>Jatropha macrantha</i> Müll Arg. on Ketamine-Induced Erectile Dysfunction in Rats |
| title_full | Phytochemical Screening by LC-ESI-MS/MS and Effect of the Ethyl Acetate Fraction from Leaves and Stems of <i>Jatropha macrantha</i> Müll Arg. on Ketamine-Induced Erectile Dysfunction in Rats |
| title_fullStr | Phytochemical Screening by LC-ESI-MS/MS and Effect of the Ethyl Acetate Fraction from Leaves and Stems of <i>Jatropha macrantha</i> Müll Arg. on Ketamine-Induced Erectile Dysfunction in Rats |
| title_full_unstemmed | Phytochemical Screening by LC-ESI-MS/MS and Effect of the Ethyl Acetate Fraction from Leaves and Stems of <i>Jatropha macrantha</i> Müll Arg. on Ketamine-Induced Erectile Dysfunction in Rats |
| title_short | Phytochemical Screening by LC-ESI-MS/MS and Effect of the Ethyl Acetate Fraction from Leaves and Stems of <i>Jatropha macrantha</i> Müll Arg. on Ketamine-Induced Erectile Dysfunction in Rats |
| title_sort | phytochemical screening by lc esi ms ms and effect of the ethyl acetate fraction from leaves and stems of i jatropha macrantha i mull arg on ketamine induced erectile dysfunction in rats |
| topic | antioxidant agent aphrodisiac liquid chromatography huanarpo sexual behavior experimental model |
| url | https://www.mdpi.com/1420-3049/27/1/115 |
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