Evaluation of in vitro antiplasmodial, antiproliferative activities, and in vivo oral acute toxicity of Spathodea campanulata flowers
Spathodea campanulata is used in traditional medicine to treat various ailments such as malaria, human immunodeficiency virus (HIV), cancer, fever and urethral inflammation. The aim of this study was to investigate the antiplasmodial, and antiproliferative activities of the extract and resulted frac...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Scientific African |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468227623003265 |
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| author | Jean Emmanuel Mbosso Teinkela Hassan Oumarou Xavier Siwe Noundou Franck Meyer Véronique Megalizzi Heinrich C. Hoppe Rui Werner Macedo Krause René Wintjens |
| author_facet | Jean Emmanuel Mbosso Teinkela Hassan Oumarou Xavier Siwe Noundou Franck Meyer Véronique Megalizzi Heinrich C. Hoppe Rui Werner Macedo Krause René Wintjens |
| author_sort | Jean Emmanuel Mbosso Teinkela |
| collection | DOAJ |
| description | Spathodea campanulata is used in traditional medicine to treat various ailments such as malaria, human immunodeficiency virus (HIV), cancer, fever and urethral inflammation. The aim of this study was to investigate the antiplasmodial, and antiproliferative activities of the extract and resulted fractions from S. campanulata flowers, as well as assessing the acute toxicity of its aqueous fraction. The in vitro cell-growth inhibition activities were assessed against Plasmodium falciparum strain 3D7 for antimalarial activity and three cancer cell lines: Hs683 (human oligodendroglioma), MCF7 (human breast carcinoma), and murine B16F10 (mouse melanoma) for antiproliferative activity while the in vivo acute oral toxicity was determined according to the modified organisation for Economic Co-operation and Development (OECD) guidelines 423 at a fixed dose on Female Wistar strain laboratory rats. The dichloromethane, ethyl acetate and hexane fractions at a concentration of 25 µg/mL each significantly reduced the viability of 3D7 Plasmodium cells with viability percentages of 19.0%, 14.1% and 31.9%, respectively, and IC50 of 28.1, 30.2 and 29.7 µg/mL, respectively. The ethyl acetate fraction showed a moderate antiproliferative activity on mouse melanoma with an I50 value of 54.6 µg/mL. Only the dichloromethane fraction was able to inhibit the 3 cell lines tested with IC50 values less than 15 µg/mL. An oral administration of the aqueous fraction did not induce an abnormal variation of the physiological parameters in female Wistar laboratory rats, at non-toxic doses up to 5000 mg/kg body weight for 14 days. These results confirm the use of this plant in traditional medicine for its antimalarial and anticancer potential. |
| first_indexed | 2024-03-11T22:26:19Z |
| format | Article |
| id | doaj.art-de73d7a93e5a4c52a0f83625f719b8ac |
| institution | Directory Open Access Journal |
| issn | 2468-2276 |
| language | English |
| last_indexed | 2024-03-11T22:26:19Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Scientific African |
| spelling | doaj.art-de73d7a93e5a4c52a0f83625f719b8ac2023-09-24T05:16:27ZengElsevierScientific African2468-22762023-09-0121e01871Evaluation of in vitro antiplasmodial, antiproliferative activities, and in vivo oral acute toxicity of Spathodea campanulata flowersJean Emmanuel Mbosso Teinkela0Hassan Oumarou1Xavier Siwe Noundou2Franck Meyer3Véronique Megalizzi4Heinrich C. Hoppe5Rui Werner Macedo Krause6René Wintjens7Microbiology, Bioorganic and Macromolecular Chemistry unit, RD3 Department, Faculté de Pharmacie, Université Libre de Bruxelles (ULB), Belgium; Department of Biological Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, P. O. Box 2701 Douala, Cameroon; Corresponding author at: Faculty of Medicine and Pharmaceutical Sciences, University of Douala, University of Douala, P. O. Box 2701, Douala, Cameroon.Department of Biological Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, P. O. Box 2701 Douala, CameroonDepartment of Pharmaceutical Sciences, School of Pharmacy, Sefako Makgatho Health Sciences University, P.O. Box 218 MEDUNSA, Pretoria 0204, South AfricaMicrobiology, Bioorganic and Macromolecular Chemistry unit, RD3 Department, Faculté de Pharmacie, Université Libre de Bruxelles (ULB), BelgiumMicrobiology, Bioorganic and Macromolecular Chemistry unit, RD3 Department, Faculté de Pharmacie, Université Libre de Bruxelles (ULB), BelgiumDepartment of Biochemistry and Microbiology, Rhodes University, South AfricaNanomaterials and Medicinal Organic Chemistry Laboratory, Department of Chemistry, Faculty of Science, Rhodes University, South AfricaMicrobiology, Bioorganic and Macromolecular Chemistry unit, RD3 Department, Faculté de Pharmacie, Université Libre de Bruxelles (ULB), BelgiumSpathodea campanulata is used in traditional medicine to treat various ailments such as malaria, human immunodeficiency virus (HIV), cancer, fever and urethral inflammation. The aim of this study was to investigate the antiplasmodial, and antiproliferative activities of the extract and resulted fractions from S. campanulata flowers, as well as assessing the acute toxicity of its aqueous fraction. The in vitro cell-growth inhibition activities were assessed against Plasmodium falciparum strain 3D7 for antimalarial activity and three cancer cell lines: Hs683 (human oligodendroglioma), MCF7 (human breast carcinoma), and murine B16F10 (mouse melanoma) for antiproliferative activity while the in vivo acute oral toxicity was determined according to the modified organisation for Economic Co-operation and Development (OECD) guidelines 423 at a fixed dose on Female Wistar strain laboratory rats. The dichloromethane, ethyl acetate and hexane fractions at a concentration of 25 µg/mL each significantly reduced the viability of 3D7 Plasmodium cells with viability percentages of 19.0%, 14.1% and 31.9%, respectively, and IC50 of 28.1, 30.2 and 29.7 µg/mL, respectively. The ethyl acetate fraction showed a moderate antiproliferative activity on mouse melanoma with an I50 value of 54.6 µg/mL. Only the dichloromethane fraction was able to inhibit the 3 cell lines tested with IC50 values less than 15 µg/mL. An oral administration of the aqueous fraction did not induce an abnormal variation of the physiological parameters in female Wistar laboratory rats, at non-toxic doses up to 5000 mg/kg body weight for 14 days. These results confirm the use of this plant in traditional medicine for its antimalarial and anticancer potential.http://www.sciencedirect.com/science/article/pii/S2468227623003265Spathodea campanulataPhytochemical analysisAntiplasmodial activityAntiproliferative activityAcute toxicity |
| spellingShingle | Jean Emmanuel Mbosso Teinkela Hassan Oumarou Xavier Siwe Noundou Franck Meyer Véronique Megalizzi Heinrich C. Hoppe Rui Werner Macedo Krause René Wintjens Evaluation of in vitro antiplasmodial, antiproliferative activities, and in vivo oral acute toxicity of Spathodea campanulata flowers Scientific African Spathodea campanulata Phytochemical analysis Antiplasmodial activity Antiproliferative activity Acute toxicity |
| title | Evaluation of in vitro antiplasmodial, antiproliferative activities, and in vivo oral acute toxicity of Spathodea campanulata flowers |
| title_full | Evaluation of in vitro antiplasmodial, antiproliferative activities, and in vivo oral acute toxicity of Spathodea campanulata flowers |
| title_fullStr | Evaluation of in vitro antiplasmodial, antiproliferative activities, and in vivo oral acute toxicity of Spathodea campanulata flowers |
| title_full_unstemmed | Evaluation of in vitro antiplasmodial, antiproliferative activities, and in vivo oral acute toxicity of Spathodea campanulata flowers |
| title_short | Evaluation of in vitro antiplasmodial, antiproliferative activities, and in vivo oral acute toxicity of Spathodea campanulata flowers |
| title_sort | evaluation of in vitro antiplasmodial antiproliferative activities and in vivo oral acute toxicity of spathodea campanulata flowers |
| topic | Spathodea campanulata Phytochemical analysis Antiplasmodial activity Antiproliferative activity Acute toxicity |
| url | http://www.sciencedirect.com/science/article/pii/S2468227623003265 |
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