Exploring effects of betaine-homocysteine methyltransferase (BHMT) gene polymorphisms on fatty acid traits and cholesterol in sheep
This study was aimed to explore the effects of Betaine-Homocysteine Methyltransferase (BHMT) gene polymorphisms on fatty acid traits and cholesterol in lambs. This study used a total of 147 blood samples for genotyping including 19 Javanese Fat-Tailed (JFT), 16 Javanese Thin-Tailed (JTT), 41 Composi...
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| Format: | Article |
| Language: | English |
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Diponegoro University, Faculty of Animal and Agricultural Sciences
2019-09-01
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| Series: | Journal of the Indonesian Tropical Animal Agriculture |
| Subjects: | |
| Online Access: | https://ejournal.undip.ac.id/index.php/jitaa/article/view/22900 |
| _version_ | 1818149693398450176 |
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| author | J. P. Munyaneza A. Gunawan R. R. Noor |
| author_facet | J. P. Munyaneza A. Gunawan R. R. Noor |
| author_sort | J. P. Munyaneza |
| collection | DOAJ |
| description | This study was aimed to explore the effects of Betaine-Homocysteine Methyltransferase (BHMT) gene polymorphisms on fatty acid traits and cholesterol in lambs. This study used a total of 147 blood samples for genotyping including 19 Javanese Fat-Tailed (JFT), 16 Javanese Thin-Tailed (JTT), 41 Composite Garut (CG), 35 Compass Agrinak (CA) and 36 Barbados Black Belly Cross (BC). A total of 61 rams as representative from five breed of sheep were selected for association study. Identification of BHMT single nucleotide polymorphisms was analyzed by PCR-RFLP method. Association of BHMT genotypes with fatty acid traits and cholesterol was performed by T-TEST. BHMT genotyping resulted into three genotypes (CC, CT and TT). Gene frequency of BHMT (g. 9947372 C>T) was in Hardy-Weinberg Equilibrium, excluding Javanese Fat-Tailed sheep. Association of BHMT genotypes with fatty acid traits resulted into a significant association (P<0.05) with C14:1, C17:1 and C18:0 fatty acids but not with cholesterol in sheep. SNP g. 9947372 (C>T) of BHMT gene might be a useful marker for selecting and producing sheep meat with desirable fatty acids. |
| first_indexed | 2024-12-11T13:11:05Z |
| format | Article |
| id | doaj.art-de7592f51cc342248345cb5fdf0affc8 |
| institution | Directory Open Access Journal |
| issn | 2087-8273 2460-6278 |
| language | English |
| last_indexed | 2024-12-11T13:11:05Z |
| publishDate | 2019-09-01 |
| publisher | Diponegoro University, Faculty of Animal and Agricultural Sciences |
| record_format | Article |
| series | Journal of the Indonesian Tropical Animal Agriculture |
| spelling | doaj.art-de7592f51cc342248345cb5fdf0affc82022-12-22T01:06:10ZengDiponegoro University, Faculty of Animal and Agricultural SciencesJournal of the Indonesian Tropical Animal Agriculture2087-82732460-62782019-09-0144324325110.14710/jitaa.44.3.243-25115471Exploring effects of betaine-homocysteine methyltransferase (BHMT) gene polymorphisms on fatty acid traits and cholesterol in sheepJ. P. Munyaneza0A. Gunawan1R. R. Noor2Graduate School of IPB University, IPB University Jl.Agatis, Dramaga Campus, Bogor 16680Department of Animal Production and Technology, Faculty of Animal Science, IPB University Jl.Agatis, Dramaga Campus, Bogor 16680Department of Animal Production and Technology, Faculty of Animal Science, IPB University Jl.Agatis, Dramaga Campus, Bogor 16680This study was aimed to explore the effects of Betaine-Homocysteine Methyltransferase (BHMT) gene polymorphisms on fatty acid traits and cholesterol in lambs. This study used a total of 147 blood samples for genotyping including 19 Javanese Fat-Tailed (JFT), 16 Javanese Thin-Tailed (JTT), 41 Composite Garut (CG), 35 Compass Agrinak (CA) and 36 Barbados Black Belly Cross (BC). A total of 61 rams as representative from five breed of sheep were selected for association study. Identification of BHMT single nucleotide polymorphisms was analyzed by PCR-RFLP method. Association of BHMT genotypes with fatty acid traits and cholesterol was performed by T-TEST. BHMT genotyping resulted into three genotypes (CC, CT and TT). Gene frequency of BHMT (g. 9947372 C>T) was in Hardy-Weinberg Equilibrium, excluding Javanese Fat-Tailed sheep. Association of BHMT genotypes with fatty acid traits resulted into a significant association (P<0.05) with C14:1, C17:1 and C18:0 fatty acids but not with cholesterol in sheep. SNP g. 9947372 (C>T) of BHMT gene might be a useful marker for selecting and producing sheep meat with desirable fatty acids.https://ejournal.undip.ac.id/index.php/jitaa/article/view/22900BHMTFatty acidSheepSNP |
| spellingShingle | J. P. Munyaneza A. Gunawan R. R. Noor Exploring effects of betaine-homocysteine methyltransferase (BHMT) gene polymorphisms on fatty acid traits and cholesterol in sheep Journal of the Indonesian Tropical Animal Agriculture BHMT Fatty acid Sheep SNP |
| title | Exploring effects of betaine-homocysteine methyltransferase (BHMT) gene polymorphisms on fatty acid traits and cholesterol in sheep |
| title_full | Exploring effects of betaine-homocysteine methyltransferase (BHMT) gene polymorphisms on fatty acid traits and cholesterol in sheep |
| title_fullStr | Exploring effects of betaine-homocysteine methyltransferase (BHMT) gene polymorphisms on fatty acid traits and cholesterol in sheep |
| title_full_unstemmed | Exploring effects of betaine-homocysteine methyltransferase (BHMT) gene polymorphisms on fatty acid traits and cholesterol in sheep |
| title_short | Exploring effects of betaine-homocysteine methyltransferase (BHMT) gene polymorphisms on fatty acid traits and cholesterol in sheep |
| title_sort | exploring effects of betaine homocysteine methyltransferase bhmt gene polymorphisms on fatty acid traits and cholesterol in sheep |
| topic | BHMT Fatty acid Sheep SNP |
| url | https://ejournal.undip.ac.id/index.php/jitaa/article/view/22900 |
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