Geographically Diverse Clusters of Nontoxigenic Corynebacterium diphtheriae Infection, Germany, 2016–2017
From 2016 through the middle of 2017, the German Consiliary Laboratory on Diphtheria noted an increase in nontoxigenic Corynebacterium diphtheriae isolates submitted from cities in northern Germany. Many patients for whom epidemiologic data were available were homeless, alcohol or drug abusers, or b...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Centers for Disease Control and Prevention
2018-07-01
|
| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/24/7/17-2026_article |
| _version_ | 1818848260413980672 |
|---|---|
| author | Alexandra Dangel Anja Berger Regina Konrad Heribert Bischoff Andreas Sing |
| author_facet | Alexandra Dangel Anja Berger Regina Konrad Heribert Bischoff Andreas Sing |
| author_sort | Alexandra Dangel |
| collection | DOAJ |
| description | From 2016 through the middle of 2017, the German Consiliary Laboratory on Diphtheria noted an increase in nontoxigenic Corynebacterium diphtheriae isolates submitted from cities in northern Germany. Many patients for whom epidemiologic data were available were homeless, alcohol or drug abusers, or both. After performing routine diagnostics and multilocus sequence typing (MLST), we analyzed isolates of sequence type (ST) 8 and previously submitted isolates by whole-genome sequencing. Results were analyzed for phylogenetic relationship by core genome MLST (cg-MLST) and whole-genome single-nucleotide polymorphism profiles. Next-generation sequencing–based cg-MLST revealed several outbreak clusters caused by ST8; the geographic focus was in the metropolitan areas of Hamburg and Berlin. To achieve enhanced analytical depth, we used additional cg-MLST target genes and genome-wide single-nucleotide polymorphisms. We identified patient characteristics and detected transmission events, providing evidence that nontoxigenic C. diphtheriae infection is a potential public health threat in industrialized countries. |
| first_indexed | 2024-12-19T06:14:31Z |
| format | Article |
| id | doaj.art-de7882640f004548860b3fdf1d9a9eb1 |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-12-19T06:14:31Z |
| publishDate | 2018-07-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-de7882640f004548860b3fdf1d9a9eb12022-12-21T20:32:54ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592018-07-012471239124510.3201/eid2407.172026Geographically Diverse Clusters of Nontoxigenic Corynebacterium diphtheriae Infection, Germany, 2016–2017Alexandra DangelAnja BergerRegina KonradHeribert BischoffAndreas SingFrom 2016 through the middle of 2017, the German Consiliary Laboratory on Diphtheria noted an increase in nontoxigenic Corynebacterium diphtheriae isolates submitted from cities in northern Germany. Many patients for whom epidemiologic data were available were homeless, alcohol or drug abusers, or both. After performing routine diagnostics and multilocus sequence typing (MLST), we analyzed isolates of sequence type (ST) 8 and previously submitted isolates by whole-genome sequencing. Results were analyzed for phylogenetic relationship by core genome MLST (cg-MLST) and whole-genome single-nucleotide polymorphism profiles. Next-generation sequencing–based cg-MLST revealed several outbreak clusters caused by ST8; the geographic focus was in the metropolitan areas of Hamburg and Berlin. To achieve enhanced analytical depth, we used additional cg-MLST target genes and genome-wide single-nucleotide polymorphisms. We identified patient characteristics and detected transmission events, providing evidence that nontoxigenic C. diphtheriae infection is a potential public health threat in industrialized countries.https://wwwnc.cdc.gov/eid/article/24/7/17-2026_articlebacterial infectionsbacteriadiphtheriadrug usersdisease outbreaksmolecular typing |
| spellingShingle | Alexandra Dangel Anja Berger Regina Konrad Heribert Bischoff Andreas Sing Geographically Diverse Clusters of Nontoxigenic Corynebacterium diphtheriae Infection, Germany, 2016–2017 Emerging Infectious Diseases bacterial infections bacteria diphtheria drug users disease outbreaks molecular typing |
| title | Geographically Diverse Clusters of Nontoxigenic Corynebacterium diphtheriae Infection, Germany, 2016–2017 |
| title_full | Geographically Diverse Clusters of Nontoxigenic Corynebacterium diphtheriae Infection, Germany, 2016–2017 |
| title_fullStr | Geographically Diverse Clusters of Nontoxigenic Corynebacterium diphtheriae Infection, Germany, 2016–2017 |
| title_full_unstemmed | Geographically Diverse Clusters of Nontoxigenic Corynebacterium diphtheriae Infection, Germany, 2016–2017 |
| title_short | Geographically Diverse Clusters of Nontoxigenic Corynebacterium diphtheriae Infection, Germany, 2016–2017 |
| title_sort | geographically diverse clusters of nontoxigenic corynebacterium diphtheriae infection germany 2016 2017 |
| topic | bacterial infections bacteria diphtheria drug users disease outbreaks molecular typing |
| url | https://wwwnc.cdc.gov/eid/article/24/7/17-2026_article |
| work_keys_str_mv | AT alexandradangel geographicallydiverseclustersofnontoxigeniccorynebacteriumdiphtheriaeinfectiongermany20162017 AT anjaberger geographicallydiverseclustersofnontoxigeniccorynebacteriumdiphtheriaeinfectiongermany20162017 AT reginakonrad geographicallydiverseclustersofnontoxigeniccorynebacteriumdiphtheriaeinfectiongermany20162017 AT heribertbischoff geographicallydiverseclustersofnontoxigeniccorynebacteriumdiphtheriaeinfectiongermany20162017 AT andreassing geographicallydiverseclustersofnontoxigeniccorynebacteriumdiphtheriaeinfectiongermany20162017 |