Characterization of <it>Pseudomonas aeruginosa </it>isolated from chronically infected children with cystic fibrosis in India
<p>Abstract</p> <p>Background</p> <p><it>Pseudomonas aeruginosa </it>is the leading cause of morbidity and mortality in patients with cystic fibrosis (CF). With chronicity of infection, the organism resides as a biofilm, shows multi-drug resistance, diversif...
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| Format: | Article |
| Language: | English |
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BMC
2005-07-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/5/43 |
| _version_ | 1828421368779112448 |
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| author | Das Bimal Kabra Susheel Kapil Arti Agarwal Gunjan Dwivedi Sada |
| author_facet | Das Bimal Kabra Susheel Kapil Arti Agarwal Gunjan Dwivedi Sada |
| author_sort | Das Bimal |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Pseudomonas aeruginosa </it>is the leading cause of morbidity and mortality in patients with cystic fibrosis (CF). With chronicity of infection, the organism resides as a biofilm, shows multi-drug resistance, diversifies its colony morphology and becomes auxotrophic. The patients have been found to be colonized with multiple genotypes. The present work was carried out to characterize <it>P. aeruginosa </it>isolated from children with cystic fibrosis using phenotypic and genotypic methods.</p> <p>Results</p> <p>We studied 56 patients with CF attending the Pediatric Chest clinic at All India Institute of Medical Sciences, New Delhi, India during August 1998-August 2001. These patients were regularly followed up at the clinic. Out of 56 patients, 27 were culture positive for <it>P. aeruginosa </it>where 8 were chronically infected (Group1) and 19 were intermittently colonized with the organism (Group2). Patients under Group1 had significantly higher rates of hospitalization, death and colonization with different colony morphotypes (p < 0.05). The isolates from Group1 patients were the positive producers of extended spectrum beta lactamase. A total of 5 auxotrophs were recovered from 2 patients where one was chronically infected with <it>P. aeruginosa </it>and the other was a recently enrolled patient. The auxotrophs had the specific requirement for methionine and arginine. Molecular typing revealed 33 ERIC-PCR (E1-E33) and 5 PCR-ribotyping (P1-P5) patterns. By ERIC-PCR, 4 patients were colonized with 2–4 genotypes and the remaining 23 patients were colonized with the single genotype.</p> <p>Conclusion</p> <p>With chronicity of infection, <it>P. aeruginosa </it>becomes multidrug resistant, diversifies its colony morphology, acquires mucoidity and shows auxotrophy for amino acids. The chronically infected patients can be colonized with multiple genotypes. Thus in a particular clinical set up, high index of suspicion should be there for diagnosis of CF patients so as to prevent the delay in diagnosis and management of CF patients.</p> |
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| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-10T15:29:22Z |
| publishDate | 2005-07-01 |
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| series | BMC Microbiology |
| spelling | doaj.art-de7987d5e7824dfc86e57a229f67c72e2022-12-22T01:43:26ZengBMCBMC Microbiology1471-21802005-07-01514310.1186/1471-2180-5-43Characterization of <it>Pseudomonas aeruginosa </it>isolated from chronically infected children with cystic fibrosis in IndiaDas BimalKabra SusheelKapil ArtiAgarwal GunjanDwivedi Sada<p>Abstract</p> <p>Background</p> <p><it>Pseudomonas aeruginosa </it>is the leading cause of morbidity and mortality in patients with cystic fibrosis (CF). With chronicity of infection, the organism resides as a biofilm, shows multi-drug resistance, diversifies its colony morphology and becomes auxotrophic. The patients have been found to be colonized with multiple genotypes. The present work was carried out to characterize <it>P. aeruginosa </it>isolated from children with cystic fibrosis using phenotypic and genotypic methods.</p> <p>Results</p> <p>We studied 56 patients with CF attending the Pediatric Chest clinic at All India Institute of Medical Sciences, New Delhi, India during August 1998-August 2001. These patients were regularly followed up at the clinic. Out of 56 patients, 27 were culture positive for <it>P. aeruginosa </it>where 8 were chronically infected (Group1) and 19 were intermittently colonized with the organism (Group2). Patients under Group1 had significantly higher rates of hospitalization, death and colonization with different colony morphotypes (p < 0.05). The isolates from Group1 patients were the positive producers of extended spectrum beta lactamase. A total of 5 auxotrophs were recovered from 2 patients where one was chronically infected with <it>P. aeruginosa </it>and the other was a recently enrolled patient. The auxotrophs had the specific requirement for methionine and arginine. Molecular typing revealed 33 ERIC-PCR (E1-E33) and 5 PCR-ribotyping (P1-P5) patterns. By ERIC-PCR, 4 patients were colonized with 2–4 genotypes and the remaining 23 patients were colonized with the single genotype.</p> <p>Conclusion</p> <p>With chronicity of infection, <it>P. aeruginosa </it>becomes multidrug resistant, diversifies its colony morphology, acquires mucoidity and shows auxotrophy for amino acids. The chronically infected patients can be colonized with multiple genotypes. Thus in a particular clinical set up, high index of suspicion should be there for diagnosis of CF patients so as to prevent the delay in diagnosis and management of CF patients.</p>http://www.biomedcentral.com/1471-2180/5/43 |
| spellingShingle | Das Bimal Kabra Susheel Kapil Arti Agarwal Gunjan Dwivedi Sada Characterization of <it>Pseudomonas aeruginosa </it>isolated from chronically infected children with cystic fibrosis in India BMC Microbiology |
| title | Characterization of <it>Pseudomonas aeruginosa </it>isolated from chronically infected children with cystic fibrosis in India |
| title_full | Characterization of <it>Pseudomonas aeruginosa </it>isolated from chronically infected children with cystic fibrosis in India |
| title_fullStr | Characterization of <it>Pseudomonas aeruginosa </it>isolated from chronically infected children with cystic fibrosis in India |
| title_full_unstemmed | Characterization of <it>Pseudomonas aeruginosa </it>isolated from chronically infected children with cystic fibrosis in India |
| title_short | Characterization of <it>Pseudomonas aeruginosa </it>isolated from chronically infected children with cystic fibrosis in India |
| title_sort | characterization of it pseudomonas aeruginosa it isolated from chronically infected children with cystic fibrosis in india |
| url | http://www.biomedcentral.com/1471-2180/5/43 |
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