Metagenomic Analysis Reveals Variations in Gut Microbiomes of the <i>Schistosoma mansoni</i>-Transmitting Snails <i>Biomphalaria straminea</i> and <i>Biomphalaria glabrata</i>
<i>Biomphalaria</i> snails play a crucial role in the transmission of the human blood fluke <i>Schistosoma mansoni</i>. The gut microbiota of intermediate hosts is known to influence their physiological functions, but little is known about its composition and role in <i>...
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MDPI AG
2023-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/10/2419 |
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| author | Peipei Li Jinni Hong Mingrou Wu Zhanhong Yuan Dinghao Li Zhongdao Wu Xi Sun Datao Lin |
| author_facet | Peipei Li Jinni Hong Mingrou Wu Zhanhong Yuan Dinghao Li Zhongdao Wu Xi Sun Datao Lin |
| author_sort | Peipei Li |
| collection | DOAJ |
| description | <i>Biomphalaria</i> snails play a crucial role in the transmission of the human blood fluke <i>Schistosoma mansoni</i>. The gut microbiota of intermediate hosts is known to influence their physiological functions, but little is known about its composition and role in <i>Biomphalaria</i> snails. To gain insights into the biological characteristics of these freshwater intermediate hosts, we conducted metagenomic sequencing on <i>Biomphalaria straminea</i> and <i>B. glabrata</i> to investigate variations in their gut microbiota. This study revealed that the dominant members of the gut microbiota in <i>B. glabrata</i> belong to the phyla Bacteroidetes and Proteobacteria, which were also found to be the top two most abundant gut bacteria in <i>B. straminea</i>. We identified Firmicutes, <i>Acidovorax</i> and <i>Bosea</i> as distinctive gut microbes in <i>B. straminea</i>, while <i>Aeromonas</i>, <i>Cloacibacterium</i> and <i>Chryseobacterium</i> were found to be dependent features of the <i>B. glabrata</i> gut microbiota. We observed significant differences in the community structures and bacterial functions of the gut microbiota between the two host species. Notably, we found a distinctive richness of antibiotic resistance genes (ARGs) associated with various classes of antibiotics, including bacitracin, chloramphenicol, tetracycline, sulfonamide, penicillin, cephalosporin_ii and cephalosporin_i, fluoroquinolone, aminoglycoside, beta-lactam, multidrug and trimethoprim, in the digestive tracts of the snails. Furthermore, this study revealed the potential correlations between snail gut microbiota and the infection rate of <i>S. mansoni</i> using Spearman correlation analysis. Through metagenomic analysis, our study provided new insights into the gut microbiota of <i>Biomphalaria</i> snails and how it is influenced by host species, thereby enhancing our understanding of variant patterns of gut microbial communities in intermediate hosts. Our findings may contribute to future studies on gastropod–microbe interactions and may provide valuable knowledge for developing snail control strategies to combat schistosomiasis in the future. |
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| institution | Directory Open Access Journal |
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| publishDate | 2023-09-01 |
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| spelling | doaj.art-de7a03cac74d45ff8f199ecc1842955a2023-11-19T17:26:27ZengMDPI AGMicroorganisms2076-26072023-09-011110241910.3390/microorganisms11102419Metagenomic Analysis Reveals Variations in Gut Microbiomes of the <i>Schistosoma mansoni</i>-Transmitting Snails <i>Biomphalaria straminea</i> and <i>Biomphalaria glabrata</i>Peipei Li0Jinni Hong1Mingrou Wu2Zhanhong Yuan3Dinghao Li4Zhongdao Wu5Xi Sun6Datao Lin7Department of Parasitology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, ChinaDepartment of Traditional Chinese Medicine, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Southern Medical University, Guangzhou 510180, ChinaDepartment of Parasitology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, ChinaDepartment of Parasitology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, ChinaDepartment of Parasitology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, ChinaDepartment of Parasitology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, ChinaDepartment of Parasitology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, ChinaDepartment of Parasitology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, China<i>Biomphalaria</i> snails play a crucial role in the transmission of the human blood fluke <i>Schistosoma mansoni</i>. The gut microbiota of intermediate hosts is known to influence their physiological functions, but little is known about its composition and role in <i>Biomphalaria</i> snails. To gain insights into the biological characteristics of these freshwater intermediate hosts, we conducted metagenomic sequencing on <i>Biomphalaria straminea</i> and <i>B. glabrata</i> to investigate variations in their gut microbiota. This study revealed that the dominant members of the gut microbiota in <i>B. glabrata</i> belong to the phyla Bacteroidetes and Proteobacteria, which were also found to be the top two most abundant gut bacteria in <i>B. straminea</i>. We identified Firmicutes, <i>Acidovorax</i> and <i>Bosea</i> as distinctive gut microbes in <i>B. straminea</i>, while <i>Aeromonas</i>, <i>Cloacibacterium</i> and <i>Chryseobacterium</i> were found to be dependent features of the <i>B. glabrata</i> gut microbiota. We observed significant differences in the community structures and bacterial functions of the gut microbiota between the two host species. Notably, we found a distinctive richness of antibiotic resistance genes (ARGs) associated with various classes of antibiotics, including bacitracin, chloramphenicol, tetracycline, sulfonamide, penicillin, cephalosporin_ii and cephalosporin_i, fluoroquinolone, aminoglycoside, beta-lactam, multidrug and trimethoprim, in the digestive tracts of the snails. Furthermore, this study revealed the potential correlations between snail gut microbiota and the infection rate of <i>S. mansoni</i> using Spearman correlation analysis. Through metagenomic analysis, our study provided new insights into the gut microbiota of <i>Biomphalaria</i> snails and how it is influenced by host species, thereby enhancing our understanding of variant patterns of gut microbial communities in intermediate hosts. Our findings may contribute to future studies on gastropod–microbe interactions and may provide valuable knowledge for developing snail control strategies to combat schistosomiasis in the future.https://www.mdpi.com/2076-2607/11/10/2419<i>Schistosoma mansoni</i>intermediate hostmetagenomicsgut microbiotavariation pattern |
| spellingShingle | Peipei Li Jinni Hong Mingrou Wu Zhanhong Yuan Dinghao Li Zhongdao Wu Xi Sun Datao Lin Metagenomic Analysis Reveals Variations in Gut Microbiomes of the <i>Schistosoma mansoni</i>-Transmitting Snails <i>Biomphalaria straminea</i> and <i>Biomphalaria glabrata</i> Microorganisms <i>Schistosoma mansoni</i> intermediate host metagenomics gut microbiota variation pattern |
| title | Metagenomic Analysis Reveals Variations in Gut Microbiomes of the <i>Schistosoma mansoni</i>-Transmitting Snails <i>Biomphalaria straminea</i> and <i>Biomphalaria glabrata</i> |
| title_full | Metagenomic Analysis Reveals Variations in Gut Microbiomes of the <i>Schistosoma mansoni</i>-Transmitting Snails <i>Biomphalaria straminea</i> and <i>Biomphalaria glabrata</i> |
| title_fullStr | Metagenomic Analysis Reveals Variations in Gut Microbiomes of the <i>Schistosoma mansoni</i>-Transmitting Snails <i>Biomphalaria straminea</i> and <i>Biomphalaria glabrata</i> |
| title_full_unstemmed | Metagenomic Analysis Reveals Variations in Gut Microbiomes of the <i>Schistosoma mansoni</i>-Transmitting Snails <i>Biomphalaria straminea</i> and <i>Biomphalaria glabrata</i> |
| title_short | Metagenomic Analysis Reveals Variations in Gut Microbiomes of the <i>Schistosoma mansoni</i>-Transmitting Snails <i>Biomphalaria straminea</i> and <i>Biomphalaria glabrata</i> |
| title_sort | metagenomic analysis reveals variations in gut microbiomes of the i schistosoma mansoni i transmitting snails i biomphalaria straminea i and i biomphalaria glabrata i |
| topic | <i>Schistosoma mansoni</i> intermediate host metagenomics gut microbiota variation pattern |
| url | https://www.mdpi.com/2076-2607/11/10/2419 |
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