A Bioinspired Astrocyte-Derived Coating Promotes the In Vitro Proliferation of Human Neural Stem Cells While Maintaining Their Stemness
The repair of neuronal tissue is a challenging process due to the limited proliferative capacity of neurons. Neural stem cells (NSCs) can aid in the regeneration process of neural tissue due to their high proliferation potential and capacity to differentiate into neurons. The therapeutic potential o...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Biomimetics |
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| Online Access: | https://www.mdpi.com/2313-7673/8/8/589 |
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| author | Andrea C. Jimenez-Vergara Jacob Avina Travis Jackson Block Anne Sheldrake Carson Koch Anna Gonzalez Jennifer Steele Ana M. Díaz-Lasprilla Dany J. Munoz-Pinto |
| author_facet | Andrea C. Jimenez-Vergara Jacob Avina Travis Jackson Block Anne Sheldrake Carson Koch Anna Gonzalez Jennifer Steele Ana M. Díaz-Lasprilla Dany J. Munoz-Pinto |
| author_sort | Andrea C. Jimenez-Vergara |
| collection | DOAJ |
| description | The repair of neuronal tissue is a challenging process due to the limited proliferative capacity of neurons. Neural stem cells (NSCs) can aid in the regeneration process of neural tissue due to their high proliferation potential and capacity to differentiate into neurons. The therapeutic potential of these cells can only be achieved if sufficient cells are obtained without losing their differentiation potential. Toward this end, an astrocyte-derived coating (HAc) was evaluated as a promising substrate to promote the proliferation of NSCs. Mass spectroscopy and scanning electron microscopy were used to characterize the HAc. The proliferation rate and the expression of stemness and differentiation markers in NSCs cultured on the HAc were evaluated and compared to the responses of these cells to commonly used coating materials including Poly-L-Ornithine (PLO), and a Human Induced Pluripotent Stem Cell (HiPSC)-based coating. The use of the HAc promotes the in vitro cell growth of NSCs. The expression of the stemness markers Sox2 and Nestin, and the differentiation marker DCX in the HAc group was akin to the expression of these markers in the controls. In summary, HAc supported the proliferation of NSCs while maintaining their stemness and neural differentiation potential. |
| first_indexed | 2024-03-08T20:58:11Z |
| format | Article |
| id | doaj.art-de7fd80828304ad5ad59a36c1296ac4b |
| institution | Directory Open Access Journal |
| issn | 2313-7673 |
| language | English |
| last_indexed | 2024-03-08T20:58:11Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomimetics |
| spelling | doaj.art-de7fd80828304ad5ad59a36c1296ac4b2023-12-22T13:55:35ZengMDPI AGBiomimetics2313-76732023-12-018858910.3390/biomimetics8080589A Bioinspired Astrocyte-Derived Coating Promotes the In Vitro Proliferation of Human Neural Stem Cells While Maintaining Their StemnessAndrea C. Jimenez-Vergara0Jacob Avina1Travis Jackson Block2Anne Sheldrake3Carson Koch4Anna Gonzalez5Jennifer Steele6Ana M. Díaz-Lasprilla7Dany J. Munoz-Pinto8Engineering Science Department, Trinity University, San Antonio, TX 78212, USAEngineering Science Department, Trinity University, San Antonio, TX 78212, USAStemBioSys, San Antonio, TX 78229, USAStemBioSys, San Antonio, TX 78229, USANeuroscience Program, Trinity University, San Antonio, TX 78212, USAEngineering Science Department, Trinity University, San Antonio, TX 78212, USAPhysics and Astronomy Department, Trinity University, San Antonio, TX 78212, USAEngineering Science Department, Trinity University, San Antonio, TX 78212, USAEngineering Science Department, Trinity University, San Antonio, TX 78212, USAThe repair of neuronal tissue is a challenging process due to the limited proliferative capacity of neurons. Neural stem cells (NSCs) can aid in the regeneration process of neural tissue due to their high proliferation potential and capacity to differentiate into neurons. The therapeutic potential of these cells can only be achieved if sufficient cells are obtained without losing their differentiation potential. Toward this end, an astrocyte-derived coating (HAc) was evaluated as a promising substrate to promote the proliferation of NSCs. Mass spectroscopy and scanning electron microscopy were used to characterize the HAc. The proliferation rate and the expression of stemness and differentiation markers in NSCs cultured on the HAc were evaluated and compared to the responses of these cells to commonly used coating materials including Poly-L-Ornithine (PLO), and a Human Induced Pluripotent Stem Cell (HiPSC)-based coating. The use of the HAc promotes the in vitro cell growth of NSCs. The expression of the stemness markers Sox2 and Nestin, and the differentiation marker DCX in the HAc group was akin to the expression of these markers in the controls. In summary, HAc supported the proliferation of NSCs while maintaining their stemness and neural differentiation potential.https://www.mdpi.com/2313-7673/8/8/589human neural stem cellsastrocyte-based coatingsproliferationstemness |
| spellingShingle | Andrea C. Jimenez-Vergara Jacob Avina Travis Jackson Block Anne Sheldrake Carson Koch Anna Gonzalez Jennifer Steele Ana M. Díaz-Lasprilla Dany J. Munoz-Pinto A Bioinspired Astrocyte-Derived Coating Promotes the In Vitro Proliferation of Human Neural Stem Cells While Maintaining Their Stemness Biomimetics human neural stem cells astrocyte-based coatings proliferation stemness |
| title | A Bioinspired Astrocyte-Derived Coating Promotes the In Vitro Proliferation of Human Neural Stem Cells While Maintaining Their Stemness |
| title_full | A Bioinspired Astrocyte-Derived Coating Promotes the In Vitro Proliferation of Human Neural Stem Cells While Maintaining Their Stemness |
| title_fullStr | A Bioinspired Astrocyte-Derived Coating Promotes the In Vitro Proliferation of Human Neural Stem Cells While Maintaining Their Stemness |
| title_full_unstemmed | A Bioinspired Astrocyte-Derived Coating Promotes the In Vitro Proliferation of Human Neural Stem Cells While Maintaining Their Stemness |
| title_short | A Bioinspired Astrocyte-Derived Coating Promotes the In Vitro Proliferation of Human Neural Stem Cells While Maintaining Their Stemness |
| title_sort | bioinspired astrocyte derived coating promotes the in vitro proliferation of human neural stem cells while maintaining their stemness |
| topic | human neural stem cells astrocyte-based coatings proliferation stemness |
| url | https://www.mdpi.com/2313-7673/8/8/589 |
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