THE CHARACTERISATION OF FUNGI Passalora fulva (Cooke) U.Braun&Crous PATHOTYPES, CAUSER OF TOMATO LEAF MOLD IN CROATIA
Tomato is considered one of the most widely grown vegetable crops in the world and production of it is rising every year in Croatia as well as in the world. This research paper shows results on tomato leaf mould caused by Passalora fulva (Cooke) U.Braun & Crous (syn. Cladosporium fulvum (Co...
| Main Author: | |
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| Format: | Article |
| Language: | English |
| Published: |
Faculty of Agrobitechnical Sciences Osijek
2012-12-01
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| Series: | Poljoprivreda |
| Subjects: | |
| Online Access: | http://161.53.194.251/~poljo/upload/publications/poljoprivreda-18-2-15.pdf |
| _version_ | 1831728828613918720 |
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| author | Adrijana Novak |
| author_facet | Adrijana Novak |
| author_sort | Adrijana Novak |
| collection | DOAJ |
| description | Tomato is considered one of the most widely
grown vegetable crops in the world and production of
it is rising every year in Croatia as well as in the world.
This research paper shows results on tomato leaf mould
caused by Passalora fulva (Cooke) U.Braun & Crous
(syn. Cladosporium fulvum (Cooke)), which can cause
significant reduction of yield and quality of tomato.
Other than economic loss, tomato leaf mould can be the
cause of allergies because it produces lots of spores.
During research, the following materials and methods
have been used: collecting samples of plant material
infected with tomato leaf mould and assessments of
disease intensity, isolation and determination of the
pathogen, detecting pathotypes using biassay, susceptibility
testing of different tomato genotypes infected
with P. fulva, molecular identification and testing the
effects of essential oils in vitro on conidia germination.
Visual controls of greenhouse and outdoor growing
tomato were conducted during the period from 2006
to 2011. Samples (17) from 14 localities in 10 counties
(Istra County, Koprivnica-Križevci County, KrapinaZagorje
County, Međimurje County, Osijek-BaranjaCounty, Primorje-Gorski Kotar County, Varaždin County,
Virovitica-Podravina County, Zadar County and Zagreb
County) were collected. Symptoms were detected on
6 different tomato genotypes: Belle, Jeremy, Minaret,
Amaneta, Gravitet and test genotype. Outdoors, P. fulva
was not found. For isolation and determination of P.
fulva 4 different growing media were used: PDA (potato
– dextrose agar), tomato – agar, carrot – agar and V8 –
agar. By measuring the size of conidia on carrot – agar
it was determined that they were the closest in size to
the conidia from infected tomato leaf. Bioassay for the
first time in Croatia confirmed presence of two different
physiological pathotypes of P. fulva, Cf – 0 and Cf – 5.
Other pathotypes obtained by bioassay were not confirmed
by sequencing because the sequences obtained
were not satisfactory for the results interpretation.
Primary PCR confirmed the fungus P. fulva in all samples
tested. Susceptibility testing of tomato genotypes on
P. fulva infection was done for the first time in Croatia.
Susceptible genotypes were Saint Pierre, Volovsko srce,
Marglobe and S. Marzano. For the first time in Croatia,
and according to the literature available probably in the
world as well, the effects of essential oil on conidial
germination of P. fulva in vitro were tested. The most
effective oil was clove oil in the concentration of 10 µl/
ml where the percentage of germinated conidia ranged
between 9 and 21%. |
| first_indexed | 2024-12-21T06:56:23Z |
| format | Article |
| id | doaj.art-de802d0a845f4900a2d70d94b2ea1294 |
| institution | Directory Open Access Journal |
| issn | 1330-7142 1848-8080 |
| language | English |
| last_indexed | 2024-12-21T06:56:23Z |
| publishDate | 2012-12-01 |
| publisher | Faculty of Agrobitechnical Sciences Osijek |
| record_format | Article |
| series | Poljoprivreda |
| spelling | doaj.art-de802d0a845f4900a2d70d94b2ea12942022-12-21T19:12:19ZengFaculty of Agrobitechnical Sciences OsijekPoljoprivreda1330-71421848-80802012-12-011826566THE CHARACTERISATION OF FUNGI Passalora fulva (Cooke) U.Braun&Crous PATHOTYPES, CAUSER OF TOMATO LEAF MOLD IN CROATIAAdrijana Novak0Croatian Centre for Agriculture, Food and Rural Affairs - Institute for Plant Protection, Zagreb, CroatiaTomato is considered one of the most widely grown vegetable crops in the world and production of it is rising every year in Croatia as well as in the world. This research paper shows results on tomato leaf mould caused by Passalora fulva (Cooke) U.Braun & Crous (syn. Cladosporium fulvum (Cooke)), which can cause significant reduction of yield and quality of tomato. Other than economic loss, tomato leaf mould can be the cause of allergies because it produces lots of spores. During research, the following materials and methods have been used: collecting samples of plant material infected with tomato leaf mould and assessments of disease intensity, isolation and determination of the pathogen, detecting pathotypes using biassay, susceptibility testing of different tomato genotypes infected with P. fulva, molecular identification and testing the effects of essential oils in vitro on conidia germination. Visual controls of greenhouse and outdoor growing tomato were conducted during the period from 2006 to 2011. Samples (17) from 14 localities in 10 counties (Istra County, Koprivnica-Križevci County, KrapinaZagorje County, Međimurje County, Osijek-BaranjaCounty, Primorje-Gorski Kotar County, Varaždin County, Virovitica-Podravina County, Zadar County and Zagreb County) were collected. Symptoms were detected on 6 different tomato genotypes: Belle, Jeremy, Minaret, Amaneta, Gravitet and test genotype. Outdoors, P. fulva was not found. For isolation and determination of P. fulva 4 different growing media were used: PDA (potato – dextrose agar), tomato – agar, carrot – agar and V8 – agar. By measuring the size of conidia on carrot – agar it was determined that they were the closest in size to the conidia from infected tomato leaf. Bioassay for the first time in Croatia confirmed presence of two different physiological pathotypes of P. fulva, Cf – 0 and Cf – 5. Other pathotypes obtained by bioassay were not confirmed by sequencing because the sequences obtained were not satisfactory for the results interpretation. Primary PCR confirmed the fungus P. fulva in all samples tested. Susceptibility testing of tomato genotypes on P. fulva infection was done for the first time in Croatia. Susceptible genotypes were Saint Pierre, Volovsko srce, Marglobe and S. Marzano. For the first time in Croatia, and according to the literature available probably in the world as well, the effects of essential oil on conidial germination of P. fulva in vitro were tested. The most effective oil was clove oil in the concentration of 10 µl/ ml where the percentage of germinated conidia ranged between 9 and 21%.http://161.53.194.251/~poljo/upload/publications/poljoprivreda-18-2-15.pdftomatotomato leaf mouldPassalora fulvagrowing mediapathotypesPCRsusceptibility testing of tomato genotypeseffects of essential oilsCroatia |
| spellingShingle | Adrijana Novak THE CHARACTERISATION OF FUNGI Passalora fulva (Cooke) U.Braun&Crous PATHOTYPES, CAUSER OF TOMATO LEAF MOLD IN CROATIA Poljoprivreda tomato tomato leaf mould Passalora fulva growing media pathotypes PCR susceptibility testing of tomato genotypes effects of essential oils Croatia |
| title | THE CHARACTERISATION OF FUNGI Passalora fulva (Cooke) U.Braun&Crous PATHOTYPES, CAUSER OF TOMATO LEAF MOLD IN CROATIA |
| title_full | THE CHARACTERISATION OF FUNGI Passalora fulva (Cooke) U.Braun&Crous PATHOTYPES, CAUSER OF TOMATO LEAF MOLD IN CROATIA |
| title_fullStr | THE CHARACTERISATION OF FUNGI Passalora fulva (Cooke) U.Braun&Crous PATHOTYPES, CAUSER OF TOMATO LEAF MOLD IN CROATIA |
| title_full_unstemmed | THE CHARACTERISATION OF FUNGI Passalora fulva (Cooke) U.Braun&Crous PATHOTYPES, CAUSER OF TOMATO LEAF MOLD IN CROATIA |
| title_short | THE CHARACTERISATION OF FUNGI Passalora fulva (Cooke) U.Braun&Crous PATHOTYPES, CAUSER OF TOMATO LEAF MOLD IN CROATIA |
| title_sort | characterisation of fungi passalora fulva cooke u braun crous pathotypes causer of tomato leaf mold in croatia |
| topic | tomato tomato leaf mould Passalora fulva growing media pathotypes PCR susceptibility testing of tomato genotypes effects of essential oils Croatia |
| url | http://161.53.194.251/~poljo/upload/publications/poljoprivreda-18-2-15.pdf |
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