Engraftment, Fate, and Function of HoxB8-Conditional Neutrophil Progenitors in the Unconditioned Murine Host
The development and use of murine myeloid progenitor cell lines that are conditionally immortalized through expression of HoxB8 has provided a valuable tool for studies of neutrophil biology. Recent work has extended the utility of HoxB8-conditional progenitors to the in vivo setting via their trans...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2022.840894/full |
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| author | Joshua T. Cohen Michael Danise Kristina D. Hinman Kristina D. Hinman Brittany M. Neumann Renita Johnson Zachary S. Wilson Zachary S. Wilson Anna Chorzalska Patrycja M. Dubielecka Craig T. Lefort |
| author_facet | Joshua T. Cohen Michael Danise Kristina D. Hinman Kristina D. Hinman Brittany M. Neumann Renita Johnson Zachary S. Wilson Zachary S. Wilson Anna Chorzalska Patrycja M. Dubielecka Craig T. Lefort |
| author_sort | Joshua T. Cohen |
| collection | DOAJ |
| description | The development and use of murine myeloid progenitor cell lines that are conditionally immortalized through expression of HoxB8 has provided a valuable tool for studies of neutrophil biology. Recent work has extended the utility of HoxB8-conditional progenitors to the in vivo setting via their transplantation into irradiated mice. Here, we describe the isolation of HoxB8-conditional progenitor cell lines that are unique in their ability to engraft in the naïve host in the absence of conditioning of the hematopoietic niche. Our results indicate that HoxB8-conditional progenitors engraft in a β1 integrin-dependent manner and transiently generate donor-derived mature neutrophils. Furthermore, we show that neutrophils derived in vivo from transplanted HoxB8-conditional progenitors are mobilized to the periphery and recruited to sites of inflammation in a manner that depends on the C-X-C chemokine receptor 2 and β2 integrins, the same mechanisms that have been described for recruitment of endogenous primary neutrophils. Together, our studies advance the understanding of HoxB8-conditional neutrophil progenitors and describe an innovative tool that, by virtue of its ability to engraft in the naïve host, will facilitate mechanistic in vivo experimentation on neutrophils. |
| first_indexed | 2024-04-11T15:34:05Z |
| format | Article |
| id | doaj.art-de82f9ec28bc4ac29f9113161ed126b5 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-04-11T15:34:05Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-de82f9ec28bc4ac29f9113161ed126b52022-12-22T04:16:04ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2022-01-011010.3389/fcell.2022.840894840894Engraftment, Fate, and Function of HoxB8-Conditional Neutrophil Progenitors in the Unconditioned Murine HostJoshua T. Cohen0Michael Danise1Kristina D. Hinman2Kristina D. Hinman3Brittany M. Neumann4Renita Johnson5Zachary S. Wilson6Zachary S. Wilson7Anna Chorzalska8Patrycja M. Dubielecka9Craig T. Lefort10Division of Surgical Research, Department of Surgery, Rhode Island Hospital, Providence, RI, United StatesDivision of Surgical Research, Department of Surgery, Rhode Island Hospital, Providence, RI, United StatesDivision of Surgical Research, Department of Surgery, Rhode Island Hospital, Providence, RI, United StatesGraduate Program in Pathobiology, Brown University, Providence, RI, United StatesDivision of Surgical Research, Department of Surgery, Rhode Island Hospital, Providence, RI, United StatesDivision of Surgical Research, Department of Surgery, Rhode Island Hospital, Providence, RI, United StatesDivision of Surgical Research, Department of Surgery, Rhode Island Hospital, Providence, RI, United StatesGraduate Program in Pathobiology, Brown University, Providence, RI, United StatesDivision of Hematology/Oncology, Rhode Island Hospital, Providence, RI, United StatesDivision of Hematology/Oncology, Rhode Island Hospital, Providence, RI, United StatesDivision of Surgical Research, Department of Surgery, Rhode Island Hospital, Providence, RI, United StatesThe development and use of murine myeloid progenitor cell lines that are conditionally immortalized through expression of HoxB8 has provided a valuable tool for studies of neutrophil biology. Recent work has extended the utility of HoxB8-conditional progenitors to the in vivo setting via their transplantation into irradiated mice. Here, we describe the isolation of HoxB8-conditional progenitor cell lines that are unique in their ability to engraft in the naïve host in the absence of conditioning of the hematopoietic niche. Our results indicate that HoxB8-conditional progenitors engraft in a β1 integrin-dependent manner and transiently generate donor-derived mature neutrophils. Furthermore, we show that neutrophils derived in vivo from transplanted HoxB8-conditional progenitors are mobilized to the periphery and recruited to sites of inflammation in a manner that depends on the C-X-C chemokine receptor 2 and β2 integrins, the same mechanisms that have been described for recruitment of endogenous primary neutrophils. Together, our studies advance the understanding of HoxB8-conditional neutrophil progenitors and describe an innovative tool that, by virtue of its ability to engraft in the naïve host, will facilitate mechanistic in vivo experimentation on neutrophils.https://www.frontiersin.org/articles/10.3389/fcell.2022.840894/fullneutrophilshematopoietic stem and progenitor cell (HSPC)engraftmentgranulopoeisiscell therapeuticsleukocyte recruitment |
| spellingShingle | Joshua T. Cohen Michael Danise Kristina D. Hinman Kristina D. Hinman Brittany M. Neumann Renita Johnson Zachary S. Wilson Zachary S. Wilson Anna Chorzalska Patrycja M. Dubielecka Craig T. Lefort Engraftment, Fate, and Function of HoxB8-Conditional Neutrophil Progenitors in the Unconditioned Murine Host Frontiers in Cell and Developmental Biology neutrophils hematopoietic stem and progenitor cell (HSPC) engraftment granulopoeisis cell therapeutics leukocyte recruitment |
| title | Engraftment, Fate, and Function of HoxB8-Conditional Neutrophil Progenitors in the Unconditioned Murine Host |
| title_full | Engraftment, Fate, and Function of HoxB8-Conditional Neutrophil Progenitors in the Unconditioned Murine Host |
| title_fullStr | Engraftment, Fate, and Function of HoxB8-Conditional Neutrophil Progenitors in the Unconditioned Murine Host |
| title_full_unstemmed | Engraftment, Fate, and Function of HoxB8-Conditional Neutrophil Progenitors in the Unconditioned Murine Host |
| title_short | Engraftment, Fate, and Function of HoxB8-Conditional Neutrophil Progenitors in the Unconditioned Murine Host |
| title_sort | engraftment fate and function of hoxb8 conditional neutrophil progenitors in the unconditioned murine host |
| topic | neutrophils hematopoietic stem and progenitor cell (HSPC) engraftment granulopoeisis cell therapeutics leukocyte recruitment |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2022.840894/full |
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