Identification and Evolutionary Relationship of <i>Corynebacterium striatum</i> Clinical Isolates
<i>Corynebacterium striatum</i> has developed into a new community-acquired and hospital-acquired multi-drug resistance (MDR) bacterium, and is a potential target pathogen for infection control and antibacterial management projects. In this study, non-duplicate samples of inpatients were...
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MDPI AG
2022-09-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/9/1012 |
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| author | Jiao Wang Jiao Pei Mingming Liu Rui Huang Jiqiang Li Shiying Liao Jian Liang |
| author_facet | Jiao Wang Jiao Pei Mingming Liu Rui Huang Jiqiang Li Shiying Liao Jian Liang |
| author_sort | Jiao Wang |
| collection | DOAJ |
| description | <i>Corynebacterium striatum</i> has developed into a new community-acquired and hospital-acquired multi-drug resistance (MDR) bacterium, and is a potential target pathogen for infection control and antibacterial management projects. In this study, non-duplicate samples of inpatients were collected from a local central hospital. Mass spectrometry showed that 54 <i>C. striatum</i> isolates mainly appeared in secretion and sputum from 14 departments. Protein fingerprint cluster analysis showed that the isolates were divided into four groups, most of which appeared in summer. The drug resistance test showed that all strains had multi-drug resistance, with high resistance rates to lincosamides, quinolones and tetracycline detected. Further analysis of the phylogenetic tree of <i>C. striatum</i> was conducted by cloning the <i>16S rRNA</i> gene. It was found that isolates in the same department had high homology and tended to be located in the same branch or to be crossed in the same main branch. The strains in the same evolutionary branch group had the same drug resistance. Screening of site-specific recombinant elements revealed that 18 strains had <i>integrase</i> genes with the same sequence. This study shows that there may be mobile genetic elements in clinical isolates that drive gene exchange among strains, thus causing the cross-infection, spread and evolution of pathogenic bacteria in the hospital. |
| first_indexed | 2024-03-09T22:53:40Z |
| format | Article |
| id | doaj.art-de83598ce1cd486d8bc0a321cc51038b |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T22:53:40Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-de83598ce1cd486d8bc0a321cc51038b2023-11-23T18:15:57ZengMDPI AGPathogens2076-08172022-09-01119101210.3390/pathogens11091012Identification and Evolutionary Relationship of <i>Corynebacterium striatum</i> Clinical IsolatesJiao Wang0Jiao Pei1Mingming Liu2Rui Huang3Jiqiang Li4Shiying Liao5Jian Liang6School of Basic Medicine, Hubei University of Arts and Science, Xiangyang 441053, ChinaXiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang 441021, ChinaSchool of Basic Medicine, Hubei University of Arts and Science, Xiangyang 441053, ChinaSchool of Basic Medicine, Hubei University of Arts and Science, Xiangyang 441053, ChinaSchool of Basic Medicine, Hubei University of Arts and Science, Xiangyang 441053, ChinaSchool of Basic Medicine, Hubei University of Arts and Science, Xiangyang 441053, ChinaXiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang 441021, China<i>Corynebacterium striatum</i> has developed into a new community-acquired and hospital-acquired multi-drug resistance (MDR) bacterium, and is a potential target pathogen for infection control and antibacterial management projects. In this study, non-duplicate samples of inpatients were collected from a local central hospital. Mass spectrometry showed that 54 <i>C. striatum</i> isolates mainly appeared in secretion and sputum from 14 departments. Protein fingerprint cluster analysis showed that the isolates were divided into four groups, most of which appeared in summer. The drug resistance test showed that all strains had multi-drug resistance, with high resistance rates to lincosamides, quinolones and tetracycline detected. Further analysis of the phylogenetic tree of <i>C. striatum</i> was conducted by cloning the <i>16S rRNA</i> gene. It was found that isolates in the same department had high homology and tended to be located in the same branch or to be crossed in the same main branch. The strains in the same evolutionary branch group had the same drug resistance. Screening of site-specific recombinant elements revealed that 18 strains had <i>integrase</i> genes with the same sequence. This study shows that there may be mobile genetic elements in clinical isolates that drive gene exchange among strains, thus causing the cross-infection, spread and evolution of pathogenic bacteria in the hospital.https://www.mdpi.com/2076-0817/11/9/1012<i>Corynebacterium striatum</i>multi-drug resistance (MDR)MALDI-TOF MSPhylogenyrelationship between microbiota evolution and drug resistance |
| spellingShingle | Jiao Wang Jiao Pei Mingming Liu Rui Huang Jiqiang Li Shiying Liao Jian Liang Identification and Evolutionary Relationship of <i>Corynebacterium striatum</i> Clinical Isolates Pathogens <i>Corynebacterium striatum</i> multi-drug resistance (MDR) MALDI-TOF MS Phylogeny relationship between microbiota evolution and drug resistance |
| title | Identification and Evolutionary Relationship of <i>Corynebacterium striatum</i> Clinical Isolates |
| title_full | Identification and Evolutionary Relationship of <i>Corynebacterium striatum</i> Clinical Isolates |
| title_fullStr | Identification and Evolutionary Relationship of <i>Corynebacterium striatum</i> Clinical Isolates |
| title_full_unstemmed | Identification and Evolutionary Relationship of <i>Corynebacterium striatum</i> Clinical Isolates |
| title_short | Identification and Evolutionary Relationship of <i>Corynebacterium striatum</i> Clinical Isolates |
| title_sort | identification and evolutionary relationship of i corynebacterium striatum i clinical isolates |
| topic | <i>Corynebacterium striatum</i> multi-drug resistance (MDR) MALDI-TOF MS Phylogeny relationship between microbiota evolution and drug resistance |
| url | https://www.mdpi.com/2076-0817/11/9/1012 |
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