Generation of human induced pluripotent stem cell line encoding for a genetically encoded voltage indicator Arclight A242
Genetically encoded voltage indicators (GEVIs) allow for monitoring membrane potential changes in neurons and cardiomyocytes (CMs) as an alternative to patch-clamp techniques. GEVIs facilitate non-invasive, high throughput screening of electrophysiological properties of human induced pluripotent ste...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-02-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506122003300 |
| _version_ | 1797956269923893248 |
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| author | Rajesh Vivekanandan Monika Szepes Maria Elena Ricci Signorini Denys Kravchenko Johanna Kiefer Steffen Berger Veronika Fricke Gudrun Göhring Ina Gruh |
| author_facet | Rajesh Vivekanandan Monika Szepes Maria Elena Ricci Signorini Denys Kravchenko Johanna Kiefer Steffen Berger Veronika Fricke Gudrun Göhring Ina Gruh |
| author_sort | Rajesh Vivekanandan |
| collection | DOAJ |
| description | Genetically encoded voltage indicators (GEVIs) allow for monitoring membrane potential changes in neurons and cardiomyocytes (CMs) as an alternative to patch-clamp techniques. GEVIs facilitate non-invasive, high throughput screening of electrophysiological properties of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). A dual transgenic hiPSC line with Arclight A242 (GEVI) and an antibiotic resistance cardiac selection cassette was successfully generated from an earlier established hiPSC line MHHi001-A. After cardiac differentiation and selection, purified populations of CMs with constitutive GEVI expression can be utilized for studying cardiac development, disease modeling, and drug testing. |
| first_indexed | 2024-04-10T23:46:23Z |
| format | Article |
| id | doaj.art-de88aff6d4d646b58d41063209e86f21 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-04-10T23:46:23Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-de88aff6d4d646b58d41063209e86f212023-01-11T04:28:24ZengElsevierStem Cell Research1873-50612023-02-0166102981Generation of human induced pluripotent stem cell line encoding for a genetically encoded voltage indicator Arclight A242Rajesh Vivekanandan0Monika Szepes1Maria Elena Ricci Signorini2Denys Kravchenko3Johanna Kiefer4Steffen Berger5Veronika Fricke6Gudrun Göhring7Ina Gruh8Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, GermanyLeibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, GermanyLeibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, GermanyLeibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, GermanyLeibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, GermanyLeibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, GermanyLeibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, GermanyDepartment of Human Genetics, Hannover Medical School, GermanyLeibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, Germany; Corresponding author.Genetically encoded voltage indicators (GEVIs) allow for monitoring membrane potential changes in neurons and cardiomyocytes (CMs) as an alternative to patch-clamp techniques. GEVIs facilitate non-invasive, high throughput screening of electrophysiological properties of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). A dual transgenic hiPSC line with Arclight A242 (GEVI) and an antibiotic resistance cardiac selection cassette was successfully generated from an earlier established hiPSC line MHHi001-A. After cardiac differentiation and selection, purified populations of CMs with constitutive GEVI expression can be utilized for studying cardiac development, disease modeling, and drug testing.http://www.sciencedirect.com/science/article/pii/S1873506122003300 |
| spellingShingle | Rajesh Vivekanandan Monika Szepes Maria Elena Ricci Signorini Denys Kravchenko Johanna Kiefer Steffen Berger Veronika Fricke Gudrun Göhring Ina Gruh Generation of human induced pluripotent stem cell line encoding for a genetically encoded voltage indicator Arclight A242 Stem Cell Research |
| title | Generation of human induced pluripotent stem cell line encoding for a genetically encoded voltage indicator Arclight A242 |
| title_full | Generation of human induced pluripotent stem cell line encoding for a genetically encoded voltage indicator Arclight A242 |
| title_fullStr | Generation of human induced pluripotent stem cell line encoding for a genetically encoded voltage indicator Arclight A242 |
| title_full_unstemmed | Generation of human induced pluripotent stem cell line encoding for a genetically encoded voltage indicator Arclight A242 |
| title_short | Generation of human induced pluripotent stem cell line encoding for a genetically encoded voltage indicator Arclight A242 |
| title_sort | generation of human induced pluripotent stem cell line encoding for a genetically encoded voltage indicator arclight a242 |
| url | http://www.sciencedirect.com/science/article/pii/S1873506122003300 |
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