Design of a Sensitive Extracellular Vesicle Detection Method Utilizing a Surface-Functionalized Power-Free Microchip
Extracellular vesicles (EVs), which are small membrane vesicles secreted from cells into bodily fluids, are promising candidates as biomarkers for various diseases. We propose a simple, highly sensitive method for detecting EVs using a microchip. The limit of detection (LOD) for EVs was improved 29-...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Membranes |
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| Online Access: | https://www.mdpi.com/2077-0375/12/7/679 |
| _version_ | 1827605114120044544 |
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| author | Ryo Ishihara Asuka Katagiri Tadaaki Nakajima Ryo Matsui Kazuo Hosokawa Mizuo Maeda Yasuhiro Tomooka Akihiko Kikuchi |
| author_facet | Ryo Ishihara Asuka Katagiri Tadaaki Nakajima Ryo Matsui Kazuo Hosokawa Mizuo Maeda Yasuhiro Tomooka Akihiko Kikuchi |
| author_sort | Ryo Ishihara |
| collection | DOAJ |
| description | Extracellular vesicles (EVs), which are small membrane vesicles secreted from cells into bodily fluids, are promising candidates as biomarkers for various diseases. We propose a simple, highly sensitive method for detecting EVs using a microchip. The limit of detection (LOD) for EVs was improved 29-fold by changing the microchannel structure of the microchip and by optimizing the EV detection protocols. The height of the microchannel was changed from 25 to 8 µm only at the detection region, and the time for EV capture was extended from 5 to 10 min. The LOD was 6.3 × 10<sup>10</sup> particles/mL, which is lower than the concentration of EVs in the blood. The detection time was 19 min, and the volume of EV solution used was 2.0 µL. These results indicate that an efficient supply of EVs to the detection region is effective in improving the sensitivity of EV detection. The proposed EV detection method is expected to contribute to the establishment of EV-based cancer point-of-care testing. |
| first_indexed | 2024-03-09T06:14:07Z |
| format | Article |
| id | doaj.art-de8f4e82c05b41898d01196ea5cf60de |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-09T06:14:07Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-de8f4e82c05b41898d01196ea5cf60de2023-12-03T11:55:22ZengMDPI AGMembranes2077-03752022-06-0112767910.3390/membranes12070679Design of a Sensitive Extracellular Vesicle Detection Method Utilizing a Surface-Functionalized Power-Free MicrochipRyo Ishihara0Asuka Katagiri1Tadaaki Nakajima2Ryo Matsui3Kazuo Hosokawa4Mizuo Maeda5Yasuhiro Tomooka6Akihiko Kikuchi7Faculty of Medicine, Juntendo University, Chiba 270-1695, JapanDepartment of Materials Science and Technology, Tokyo University of Science, Tokyo 125-8585, JapanDepartment of Bioengineering and Technology, Tokyo University of Science, Tokyo 125-8585, JapanDepartment of Materials Science and Technology, Tokyo University of Science, Tokyo 125-8585, JapanBioengineering Laboratory, RIKEN Cluster for Pioneering Research, RIKEN, Saitama 351-0198, JapanBioengineering Laboratory, RIKEN Cluster for Pioneering Research, RIKEN, Saitama 351-0198, JapanDepartment of Bioengineering and Technology, Tokyo University of Science, Tokyo 125-8585, JapanDepartment of Materials Science and Technology, Tokyo University of Science, Tokyo 125-8585, JapanExtracellular vesicles (EVs), which are small membrane vesicles secreted from cells into bodily fluids, are promising candidates as biomarkers for various diseases. We propose a simple, highly sensitive method for detecting EVs using a microchip. The limit of detection (LOD) for EVs was improved 29-fold by changing the microchannel structure of the microchip and by optimizing the EV detection protocols. The height of the microchannel was changed from 25 to 8 µm only at the detection region, and the time for EV capture was extended from 5 to 10 min. The LOD was 6.3 × 10<sup>10</sup> particles/mL, which is lower than the concentration of EVs in the blood. The detection time was 19 min, and the volume of EV solution used was 2.0 µL. These results indicate that an efficient supply of EVs to the detection region is effective in improving the sensitivity of EV detection. The proposed EV detection method is expected to contribute to the establishment of EV-based cancer point-of-care testing.https://www.mdpi.com/2077-0375/12/7/679surface-functionalized power-free microchipextracellular vesiclesdetection sensitivitypoint-of-care testingcancers |
| spellingShingle | Ryo Ishihara Asuka Katagiri Tadaaki Nakajima Ryo Matsui Kazuo Hosokawa Mizuo Maeda Yasuhiro Tomooka Akihiko Kikuchi Design of a Sensitive Extracellular Vesicle Detection Method Utilizing a Surface-Functionalized Power-Free Microchip Membranes surface-functionalized power-free microchip extracellular vesicles detection sensitivity point-of-care testing cancers |
| title | Design of a Sensitive Extracellular Vesicle Detection Method Utilizing a Surface-Functionalized Power-Free Microchip |
| title_full | Design of a Sensitive Extracellular Vesicle Detection Method Utilizing a Surface-Functionalized Power-Free Microchip |
| title_fullStr | Design of a Sensitive Extracellular Vesicle Detection Method Utilizing a Surface-Functionalized Power-Free Microchip |
| title_full_unstemmed | Design of a Sensitive Extracellular Vesicle Detection Method Utilizing a Surface-Functionalized Power-Free Microchip |
| title_short | Design of a Sensitive Extracellular Vesicle Detection Method Utilizing a Surface-Functionalized Power-Free Microchip |
| title_sort | design of a sensitive extracellular vesicle detection method utilizing a surface functionalized power free microchip |
| topic | surface-functionalized power-free microchip extracellular vesicles detection sensitivity point-of-care testing cancers |
| url | https://www.mdpi.com/2077-0375/12/7/679 |
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