A 24-bp Indel (Insertion-Deletion) Polymorphism in Promoter Prolactin Gene
Prolactin in chicken (cPRL) is a polypeptide hormone that plays a crucial role in incubation and brooding behavior. This hormone is coded by a gene that located at chromosome 2. The objective of this study was to investigate 24-bp indel polymorphism in promoter prolactin gene of Papua local chickens...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Universitas Jenderal Soedirman (UNSOED), Faculty of Animal Science
2016-01-01
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| Series: | Animal Production: Indonesian Journal of Animal Production |
| Online Access: | http://animalproduction.net/index.php/JAP/article/view/526 |
| Summary: | Prolactin in chicken (cPRL) is a polypeptide hormone that plays a crucial role in incubation and brooding behavior. This hormone is coded by a gene that located at chromosome 2. The objective of this study was to investigate 24-bp indel polymorphism in promoter prolactin gene of Papua local chickens. A number of 60 DNA samples came from 60 Papua local chickens (25 males and 35 females) were used in this study. PCR (Polymerase Chain Reaction) method with a pair of specific primer was applied and a specific DNA fragment with 130 and/or 154 bp contained 24-bp indel was successfully amplified. Results of electrophoresis run on 1.5 % agarose gel showed three genotypes: II, ID and DD with the frequency of 6,7%, 48.3% and 45% respectively. The frequency of I allele was 0.31 and D allele was 0.69. Results indicated that the I allele frequency was medium in Papua local chickens population. Results therefore suggest that incubation behavior in hen population of Papua local chickens is easy to eliminate by increasing the I allele frequency in the population. |
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| ISSN: | 2541-5875 2541-5875 |