Microbial nitrogen cycling in Arctic snowpacks
Arctic snowpacks are often considered as chemical reactors for a variety of chemicals deposited through wet and dry events, but are overlooked as potential sites for microbial metabolism of reactive nitrogen species. The fate of deposited species is critical since warming leads to the transfer of co...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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IOP Publishing
2013-01-01
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| Series: | Environmental Research Letters |
| Subjects: | |
| Online Access: | https://doi.org/10.1088/1748-9326/8/3/035004 |
| _version_ | 1797748168680538112 |
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| author | Catherine Larose Aurélien Dommergue Timothy M Vogel |
| author_facet | Catherine Larose Aurélien Dommergue Timothy M Vogel |
| author_sort | Catherine Larose |
| collection | DOAJ |
| description | Arctic snowpacks are often considered as chemical reactors for a variety of chemicals deposited through wet and dry events, but are overlooked as potential sites for microbial metabolism of reactive nitrogen species. The fate of deposited species is critical since warming leads to the transfer of contaminants to snowmelt-fed ecosystems. Here, we examined the role of microorganisms and the potential pathways involved in nitrogen cycling in the snow. Next generation sequencing data were used to follow functional gene abundances and a 16S rRNA (ribosomal ribonucleic acid) gene microarray was used to follow shifts in microbial community structure during a two-month spring-time field study at a high Arctic site, Svalbard, Norway (79° N). We showed that despite the low temperatures and limited water supply, microbial communities inhabiting the snow cover demonstrated dynamic shifts in their functional potential to follow several different pathways of the nitrogen cycle. In addition, microbial specific phylogenetic probes tracked different nitrogen species over time. For example, probes for Roseomonas tracked nitrate concentrations closely and probes for Caulobacter tracked ammonium concentrations after a delay of one week. Nitrogen cycling was also shown to be a dominant process at the base of the snowpack. |
| first_indexed | 2024-03-12T16:02:04Z |
| format | Article |
| id | doaj.art-de91073bb9e14778ab2f99be8fdba65b |
| institution | Directory Open Access Journal |
| issn | 1748-9326 |
| language | English |
| last_indexed | 2024-03-12T16:02:04Z |
| publishDate | 2013-01-01 |
| publisher | IOP Publishing |
| record_format | Article |
| series | Environmental Research Letters |
| spelling | doaj.art-de91073bb9e14778ab2f99be8fdba65b2023-08-09T14:37:13ZengIOP PublishingEnvironmental Research Letters1748-93262013-01-018303500410.1088/1748-9326/8/3/035004Microbial nitrogen cycling in Arctic snowpacksCatherine Larose0Aurélien Dommergue1Timothy M Vogel2Environmental Microbial Genomics, Ecole Centrale de Lyon, Université de Lyon , 36 avenue Guy de Collongue, F-69134 Ecully, FranceUniversité Joseph Fourier—Grenoble 1/CNRS , LGGE, 54 rue Molière BP56, F-38402 Saint Martin d’Hères, FranceEnvironmental Microbial Genomics, Ecole Centrale de Lyon, Université de Lyon , 36 avenue Guy de Collongue, F-69134 Ecully, FranceArctic snowpacks are often considered as chemical reactors for a variety of chemicals deposited through wet and dry events, but are overlooked as potential sites for microbial metabolism of reactive nitrogen species. The fate of deposited species is critical since warming leads to the transfer of contaminants to snowmelt-fed ecosystems. Here, we examined the role of microorganisms and the potential pathways involved in nitrogen cycling in the snow. Next generation sequencing data were used to follow functional gene abundances and a 16S rRNA (ribosomal ribonucleic acid) gene microarray was used to follow shifts in microbial community structure during a two-month spring-time field study at a high Arctic site, Svalbard, Norway (79° N). We showed that despite the low temperatures and limited water supply, microbial communities inhabiting the snow cover demonstrated dynamic shifts in their functional potential to follow several different pathways of the nitrogen cycle. In addition, microbial specific phylogenetic probes tracked different nitrogen species over time. For example, probes for Roseomonas tracked nitrate concentrations closely and probes for Caulobacter tracked ammonium concentrations after a delay of one week. Nitrogen cycling was also shown to be a dominant process at the base of the snowpack.https://doi.org/10.1088/1748-9326/8/3/035004nitrogen cyclemetagenomicsmicrobial ecology |
| spellingShingle | Catherine Larose Aurélien Dommergue Timothy M Vogel Microbial nitrogen cycling in Arctic snowpacks Environmental Research Letters nitrogen cycle metagenomics microbial ecology |
| title | Microbial nitrogen cycling in Arctic snowpacks |
| title_full | Microbial nitrogen cycling in Arctic snowpacks |
| title_fullStr | Microbial nitrogen cycling in Arctic snowpacks |
| title_full_unstemmed | Microbial nitrogen cycling in Arctic snowpacks |
| title_short | Microbial nitrogen cycling in Arctic snowpacks |
| title_sort | microbial nitrogen cycling in arctic snowpacks |
| topic | nitrogen cycle metagenomics microbial ecology |
| url | https://doi.org/10.1088/1748-9326/8/3/035004 |
| work_keys_str_mv | AT catherinelarose microbialnitrogencyclinginarcticsnowpacks AT aureliendommergue microbialnitrogencyclinginarcticsnowpacks AT timothymvogel microbialnitrogencyclinginarcticsnowpacks |