Molecular analysis of Annexin expression in cancer
Abstract Background Uptake of apoptotic cells induces a tolerogenic phenotype in phagocytes and promotes peripheral tolerance. The highly conserved Annexin core domain, present in all members of the Annexin family, becomes exposed on the apoptotic cell-surface and triggers tolerogenic signalling in...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-09-01
|
| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12885-022-10075-8 |
| _version_ | 1818016627736707072 |
|---|---|
| author | Tobias Hein Peter H. Krammer Heiko Weyd |
| author_facet | Tobias Hein Peter H. Krammer Heiko Weyd |
| author_sort | Tobias Hein |
| collection | DOAJ |
| description | Abstract Background Uptake of apoptotic cells induces a tolerogenic phenotype in phagocytes and promotes peripheral tolerance. The highly conserved Annexin core domain, present in all members of the Annexin family, becomes exposed on the apoptotic cell-surface and triggers tolerogenic signalling in phagocytes via the Dectin-1 receptor. Consequently, Annexins exposed on tumour cells upon cell death are expected to induce tolerance towards tumour antigens, inhibiting tumour rejection. Methods Expression analysis for all Annexin family members was conducted in cancer cell lines of diverse origins. Presentation of Annexins on the cell surface during apoptosis of cancer cell lines was investigated using surface washes and immunoblotting. Expression data from the GEO database was analysed to compare Annexin levels between malignant and healthy tissue. Results Six Annexins at least were consistently detected on mRNA and protein level for each investigated cell line. AnxA1, AnxA2 and AnxA5 constituted the major part of total Annexin expression. All expressed Annexins translocated to the cell surface upon apoptosis induction in all cell lines. Human expression data indicate a correlation between immune infiltration and overall Annexin expression in malignant compared to healthy tissue. Conclusions This study is the first comprehensive analysis of expression, distribution and presentation of Annexins in cancer. |
| first_indexed | 2024-04-14T07:16:23Z |
| format | Article |
| id | doaj.art-de913e4683014135a2e3a6212974b092 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-04-14T07:16:23Z |
| publishDate | 2022-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-de913e4683014135a2e3a6212974b0922022-12-22T02:06:18ZengBMCBMC Cancer1471-24072022-09-0122111410.1186/s12885-022-10075-8Molecular analysis of Annexin expression in cancerTobias Hein0Peter H. Krammer1Heiko Weyd2Division of Immunogenetics, Tumour Immunology Program, German Cancer Research CentreDivision of Immunogenetics, Tumour Immunology Program, German Cancer Research CentreDivision of Immunogenetics, Tumour Immunology Program, German Cancer Research CentreAbstract Background Uptake of apoptotic cells induces a tolerogenic phenotype in phagocytes and promotes peripheral tolerance. The highly conserved Annexin core domain, present in all members of the Annexin family, becomes exposed on the apoptotic cell-surface and triggers tolerogenic signalling in phagocytes via the Dectin-1 receptor. Consequently, Annexins exposed on tumour cells upon cell death are expected to induce tolerance towards tumour antigens, inhibiting tumour rejection. Methods Expression analysis for all Annexin family members was conducted in cancer cell lines of diverse origins. Presentation of Annexins on the cell surface during apoptosis of cancer cell lines was investigated using surface washes and immunoblotting. Expression data from the GEO database was analysed to compare Annexin levels between malignant and healthy tissue. Results Six Annexins at least were consistently detected on mRNA and protein level for each investigated cell line. AnxA1, AnxA2 and AnxA5 constituted the major part of total Annexin expression. All expressed Annexins translocated to the cell surface upon apoptosis induction in all cell lines. Human expression data indicate a correlation between immune infiltration and overall Annexin expression in malignant compared to healthy tissue. Conclusions This study is the first comprehensive analysis of expression, distribution and presentation of Annexins in cancer.https://doi.org/10.1186/s12885-022-10075-8AnnexinsApoptotic cellsTumour microenvironmentPeripheral toleranceImmunosuppression |
| spellingShingle | Tobias Hein Peter H. Krammer Heiko Weyd Molecular analysis of Annexin expression in cancer BMC Cancer Annexins Apoptotic cells Tumour microenvironment Peripheral tolerance Immunosuppression |
| title | Molecular analysis of Annexin expression in cancer |
| title_full | Molecular analysis of Annexin expression in cancer |
| title_fullStr | Molecular analysis of Annexin expression in cancer |
| title_full_unstemmed | Molecular analysis of Annexin expression in cancer |
| title_short | Molecular analysis of Annexin expression in cancer |
| title_sort | molecular analysis of annexin expression in cancer |
| topic | Annexins Apoptotic cells Tumour microenvironment Peripheral tolerance Immunosuppression |
| url | https://doi.org/10.1186/s12885-022-10075-8 |
| work_keys_str_mv | AT tobiashein molecularanalysisofannexinexpressionincancer AT peterhkrammer molecularanalysisofannexinexpressionincancer AT heikoweyd molecularanalysisofannexinexpressionincancer |