Mass cytometry reveals the corneal immune cell changes at single cell level in diabetic mice
IntroductionDiabetic ocular complications include sight-threatening consequences and decreased corneal sensitivity, characterized by decreased tear production, corneal sensitivity and delayed corneal epithelial wound healing. The pathogenesis of diabetic corneal disorders remains largely unknown. Gr...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2023.1253188/full |
| _version_ | 1797692761559793664 |
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| author | Limin Qin Limin Qin Limin Qin Qian Li Qian Li Qian Li Liqiang Wang Yifei Huang |
| author_facet | Limin Qin Limin Qin Limin Qin Qian Li Qian Li Qian Li Liqiang Wang Yifei Huang |
| author_sort | Limin Qin |
| collection | DOAJ |
| description | IntroductionDiabetic ocular complications include sight-threatening consequences and decreased corneal sensitivity, characterized by decreased tear production, corneal sensitivity and delayed corneal epithelial wound healing. The pathogenesis of diabetic corneal disorders remains largely unknown. Growing evidence implies the participation of immune cells in the development of diabetic corneal diseases. Nonetheless, the immunological changes that result in diabetic corneal problems are largely unknown.MethodsMass cytometry by time of flight (CyTOF) was used to investigate immune cell cluster alterations associated with diabetic corneal disorders. CyTOF test was performed on corneal cells at a single level from 21-week-old diabetic (db/db) and non-diabetic (db/m) mice. A panel of 41 immune-related markers monitored different immune cell types in diabetic corneas. To investigate the proportion of each immune cell subpopulation, an unsupervised clustering method was employed, and T-distributed stochastic neighbor embedding was used to visualize the distinctions between different immune cell subsets.ResultsThrough CyTOF test, we identified 10 immune cell subsets in the corneal tissues. In a novel way, we discovered significant immune alterations in diabetic corneas, including pronounced alterations in T cells and myeloid cell subgroups in diabetic corneas linked to potential biomarkers, including CD103, CCR2, SiglecF, Ly6G, and CD172a. Comprehensive immunological profiling indicated remarkable changes in the immune microenvironment in diabetic corneas, characterized by a notable decrease in CD103+CD8+ tissue-resident memory T (TRM) cells and Tregs, as well as a dramatic increase of γδT cells and subsets of CD11b+Ly6G+ myeloid-derived suppressor cells (MDSCs).ConclusionCyTOF analysis revealed significant alterations in the immune microenvironment during the development of diabetic corneal complications. This study mapped the immune microenvironment landscape of type 2 diabetic corneas, providing a fundamental understanding of immune-driven diabetic corneal disorders. |
| first_indexed | 2024-03-12T02:33:16Z |
| format | Article |
| id | doaj.art-de936c6d9ee849baab42515e1be34c4d |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-03-12T02:33:16Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-de936c6d9ee849baab42515e1be34c4d2023-09-05T06:54:04ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922023-09-011410.3389/fendo.2023.12531881253188Mass cytometry reveals the corneal immune cell changes at single cell level in diabetic miceLimin Qin0Limin Qin1Limin Qin2Qian Li3Qian Li4Qian Li5Liqiang Wang6Yifei Huang7Department of Ophthalmology, The Third Medical Center, Chinese People's Liberation Army of China General Hospital, Beijing, ChinaDepartment of Ophthalmology, The First Medical Center, Chinese People's Liberation Army of China General Hospital, Beijing, ChinaDepartment of Ophthalmology, Medical School of Chinese People’s Liberation Army (PLA), Beijing, ChinaDepartment of Ophthalmology, The Third Medical Center, Chinese People's Liberation Army of China General Hospital, Beijing, ChinaDepartment of Ophthalmology, The First Medical Center, Chinese People's Liberation Army of China General Hospital, Beijing, ChinaDepartment of Ophthalmology, Medical School of Chinese People’s Liberation Army (PLA), Beijing, ChinaDepartment of Ophthalmology, The Third Medical Center, Chinese People's Liberation Army of China General Hospital, Beijing, ChinaDepartment of Ophthalmology, The Third Medical Center, Chinese People's Liberation Army of China General Hospital, Beijing, ChinaIntroductionDiabetic ocular complications include sight-threatening consequences and decreased corneal sensitivity, characterized by decreased tear production, corneal sensitivity and delayed corneal epithelial wound healing. The pathogenesis of diabetic corneal disorders remains largely unknown. Growing evidence implies the participation of immune cells in the development of diabetic corneal diseases. Nonetheless, the immunological changes that result in diabetic corneal problems are largely unknown.MethodsMass cytometry by time of flight (CyTOF) was used to investigate immune cell cluster alterations associated with diabetic corneal disorders. CyTOF test was performed on corneal cells at a single level from 21-week-old diabetic (db/db) and non-diabetic (db/m) mice. A panel of 41 immune-related markers monitored different immune cell types in diabetic corneas. To investigate the proportion of each immune cell subpopulation, an unsupervised clustering method was employed, and T-distributed stochastic neighbor embedding was used to visualize the distinctions between different immune cell subsets.ResultsThrough CyTOF test, we identified 10 immune cell subsets in the corneal tissues. In a novel way, we discovered significant immune alterations in diabetic corneas, including pronounced alterations in T cells and myeloid cell subgroups in diabetic corneas linked to potential biomarkers, including CD103, CCR2, SiglecF, Ly6G, and CD172a. Comprehensive immunological profiling indicated remarkable changes in the immune microenvironment in diabetic corneas, characterized by a notable decrease in CD103+CD8+ tissue-resident memory T (TRM) cells and Tregs, as well as a dramatic increase of γδT cells and subsets of CD11b+Ly6G+ myeloid-derived suppressor cells (MDSCs).ConclusionCyTOF analysis revealed significant alterations in the immune microenvironment during the development of diabetic corneal complications. This study mapped the immune microenvironment landscape of type 2 diabetic corneas, providing a fundamental understanding of immune-driven diabetic corneal disorders.https://www.frontiersin.org/articles/10.3389/fendo.2023.1253188/fulltissue-resident memory Tmyeloid-derived suppressor cellsγδ T cellsimmune microenvironmenthyperglycemia |
| spellingShingle | Limin Qin Limin Qin Limin Qin Qian Li Qian Li Qian Li Liqiang Wang Yifei Huang Mass cytometry reveals the corneal immune cell changes at single cell level in diabetic mice Frontiers in Endocrinology tissue-resident memory T myeloid-derived suppressor cells γδ T cells immune microenvironment hyperglycemia |
| title | Mass cytometry reveals the corneal immune cell changes at single cell level in diabetic mice |
| title_full | Mass cytometry reveals the corneal immune cell changes at single cell level in diabetic mice |
| title_fullStr | Mass cytometry reveals the corneal immune cell changes at single cell level in diabetic mice |
| title_full_unstemmed | Mass cytometry reveals the corneal immune cell changes at single cell level in diabetic mice |
| title_short | Mass cytometry reveals the corneal immune cell changes at single cell level in diabetic mice |
| title_sort | mass cytometry reveals the corneal immune cell changes at single cell level in diabetic mice |
| topic | tissue-resident memory T myeloid-derived suppressor cells γδ T cells immune microenvironment hyperglycemia |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2023.1253188/full |
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