Penapisan Kapang Asal Lahan Sulfat Masam Kalimantan Selatan Sebagai Penghasil Enzim Ekstraseluler
Kalimantan acid sulphate land has the potential to be developed into productive land, with good land optimization. Utilization of rhizosphere microorganism diversity, especially mold can potentially provide a solution in optimizing agricultural land, namely the ability to produce extracellular enzy...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | Indonesian |
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Universitas Kristen Satya Wacana
2020-10-01
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| Series: | Agric |
| Subjects: | |
| Online Access: | https://ejournal.uksw.edu/agric/article/view/3739 |
| _version_ | 1797741089691533312 |
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| author | Dwi Ningsih Susilowati Indah Sofiana Kristina Dwi Atmini Erny Yuniarti |
| author_facet | Dwi Ningsih Susilowati Indah Sofiana Kristina Dwi Atmini Erny Yuniarti |
| author_sort | Dwi Ningsih Susilowati |
| collection | DOAJ |
| description |
Kalimantan acid sulphate land has the potential to be developed into productive land, with good land optimization. Utilization of rhizosphere microorganism diversity, especially mold can potentially provide a solution in optimizing agricultural land, namely the ability to produce extracellular enzymes. This study aims to determine the potential of mold originating from acid sulphate fields in producing extracellular enzymes (pectinase, chitinase, glucanase, cellulase, and phosphatase). The study was conducted in June-July 2019 at the Microbiology Laboratory, Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development. Screening of extracellular enzyme-producing fungi was carried out on selection media. The results obtained by some isolates have the ability to produce extracellular enzymes. Indications of the ability of mold to produce extracellular enzymes are the presence of clear zones in the selection medium. In pectinase, chitinase and glucanase testing all isolates showed negative results. Potential isolates in producing extracellular enzymes include Penicillium sp. Paddy 4.1 (cellulolytic index 2.43), Clonostachys sp. KRMT 17.9 and Penicillium singorense KLK 13.7 (proteolytic indices 3.97 and 3,00, respectively). The difference in index values indicates the variation in the level of enzyme activity.
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| first_indexed | 2024-03-12T14:21:52Z |
| format | Article |
| id | doaj.art-de95075143ef444f87c34916bea2756f |
| institution | Directory Open Access Journal |
| issn | 0854-9028 2549-9343 |
| language | Indonesian |
| last_indexed | 2024-03-12T14:21:52Z |
| publishDate | 2020-10-01 |
| publisher | Universitas Kristen Satya Wacana |
| record_format | Article |
| series | Agric |
| spelling | doaj.art-de95075143ef444f87c34916bea2756f2023-08-19T02:44:08ZindUniversitas Kristen Satya WacanaAgric0854-90282549-93432020-10-0132110.24246/agric.2020.v32.i1.p65-82Penapisan Kapang Asal Lahan Sulfat Masam Kalimantan Selatan Sebagai Penghasil Enzim EkstraselulerDwi Ningsih Susilowati0Indah Sofiana1Kristina Dwi Atmini2Erny Yuniarti3BB. BiogenDepartemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri JakartaBalai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik PertanianBalai Penelitian Tanah Kalimantan acid sulphate land has the potential to be developed into productive land, with good land optimization. Utilization of rhizosphere microorganism diversity, especially mold can potentially provide a solution in optimizing agricultural land, namely the ability to produce extracellular enzymes. This study aims to determine the potential of mold originating from acid sulphate fields in producing extracellular enzymes (pectinase, chitinase, glucanase, cellulase, and phosphatase). The study was conducted in June-July 2019 at the Microbiology Laboratory, Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development. Screening of extracellular enzyme-producing fungi was carried out on selection media. The results obtained by some isolates have the ability to produce extracellular enzymes. Indications of the ability of mold to produce extracellular enzymes are the presence of clear zones in the selection medium. In pectinase, chitinase and glucanase testing all isolates showed negative results. Potential isolates in producing extracellular enzymes include Penicillium sp. Paddy 4.1 (cellulolytic index 2.43), Clonostachys sp. KRMT 17.9 and Penicillium singorense KLK 13.7 (proteolytic indices 3.97 and 3,00, respectively). The difference in index values indicates the variation in the level of enzyme activity. https://ejournal.uksw.edu/agric/article/view/3739acid sulfate fieldmoldextracellular enzymes |
| spellingShingle | Dwi Ningsih Susilowati Indah Sofiana Kristina Dwi Atmini Erny Yuniarti Penapisan Kapang Asal Lahan Sulfat Masam Kalimantan Selatan Sebagai Penghasil Enzim Ekstraseluler Agric acid sulfate field mold extracellular enzymes |
| title | Penapisan Kapang Asal Lahan Sulfat Masam Kalimantan Selatan Sebagai Penghasil Enzim Ekstraseluler |
| title_full | Penapisan Kapang Asal Lahan Sulfat Masam Kalimantan Selatan Sebagai Penghasil Enzim Ekstraseluler |
| title_fullStr | Penapisan Kapang Asal Lahan Sulfat Masam Kalimantan Selatan Sebagai Penghasil Enzim Ekstraseluler |
| title_full_unstemmed | Penapisan Kapang Asal Lahan Sulfat Masam Kalimantan Selatan Sebagai Penghasil Enzim Ekstraseluler |
| title_short | Penapisan Kapang Asal Lahan Sulfat Masam Kalimantan Selatan Sebagai Penghasil Enzim Ekstraseluler |
| title_sort | penapisan kapang asal lahan sulfat masam kalimantan selatan sebagai penghasil enzim ekstraseluler |
| topic | acid sulfate field mold extracellular enzymes |
| url | https://ejournal.uksw.edu/agric/article/view/3739 |
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