Metallo-β-lactamase and AmpC genes in Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa isolates from abattoir and poultry origin in Nigeria
Abstract Background Gram-negative bacteria (GNB) including Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae represent the most relevant reservoir of resistance genes such as metallo-β-lactamase (MBL) and AmpC genes that give them the undue advantage to resist antimicrobial onslaug...
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| Format: | Article |
| Language: | English |
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BMC
2021-04-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-021-02179-1 |
| _version_ | 1818961229678379008 |
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| author | Chika Ejikeugwu Okoro Nworie Morteza Saki Hussein O. M. Al-Dahmoshi Noor S. K. Al-Khafaji Chika Ezeador Emmanuel Nwakaeze Peter Eze Eniola Oni Chidiebere Obi Ifeanyichukwu Iroha Charles Esimone Michael U. Adikwu |
| author_facet | Chika Ejikeugwu Okoro Nworie Morteza Saki Hussein O. M. Al-Dahmoshi Noor S. K. Al-Khafaji Chika Ezeador Emmanuel Nwakaeze Peter Eze Eniola Oni Chidiebere Obi Ifeanyichukwu Iroha Charles Esimone Michael U. Adikwu |
| author_sort | Chika Ejikeugwu |
| collection | DOAJ |
| description | Abstract Background Gram-negative bacteria (GNB) including Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae represent the most relevant reservoir of resistance genes such as metallo-β-lactamase (MBL) and AmpC genes that give them the undue advantage to resist antimicrobial onslaught. This study aimed to investigate the occurrence of MBL (bla IMP-1, bla IMP-2, bla VIM-1, bla VIM-2) and AmpC (bla FOX, bla DHA, bla CMY, bla ACC) resistance genes in aforementioned GNB collected from abattoir and poultry sources in Nigeria. Results In total, 370 isolates were collected from abattoir tables (n = 130), anal region of cows (n = 120), and the cloacae of poultry birds (n = 120). The test isolates showed high rate of resistance to cephalosporins and carbapenems. The MBLs were phenotypically detected in 22 E. coli, 22 P. aeruginosa, and 18 K. pneumoniae isolates using combined disc test (CDT). However, only 11 E. coli, 24 P. aeruginosa, and 18 Klebsiella pneumoniae isolates were phenotypically confirmed to be AmpC producers using cefoxitin-cloxacillin double disk synergy test (CC-DDST). MBL encoding genes (particularly the bla IMP-1 genes and bla IMP-2 genes) were detected by polymerase chain reaction (PCR) in 12 (54.6%) E. coli, 15 (83.3%) K. pneumoniae, and 16 (72.7%) P. aeruginosa isolates. AmpC genes (particularly the bla CMY genes and bla FOX genes) were found in a total of 5 (29.4%) E. coli isolates, 5 (27.8%) isolates of K. pneumoniae, and 10 (41.7%) isolates of P. aeruginosa. Conclusions Our study showed the circulation of MBL and AmpC genes in GNB from abattoir and poultry origin in Nigeria. Adoption of regular control policies is necessary to reduce the spread of these species as soon as possible, especially in poultry and slaughterhouses. |
| first_indexed | 2024-12-20T12:10:07Z |
| format | Article |
| id | doaj.art-de952b78901b4094bf453e76b0dca2e1 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-20T12:10:07Z |
| publishDate | 2021-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-de952b78901b4094bf453e76b0dca2e12022-12-21T19:41:16ZengBMCBMC Microbiology1471-21802021-04-012111910.1186/s12866-021-02179-1Metallo-β-lactamase and AmpC genes in Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa isolates from abattoir and poultry origin in NigeriaChika Ejikeugwu0Okoro Nworie1Morteza Saki2Hussein O. M. Al-Dahmoshi3Noor S. K. Al-Khafaji4Chika Ezeador5Emmanuel Nwakaeze6Peter Eze7Eniola Oni8Chidiebere Obi9Ifeanyichukwu Iroha10Charles Esimone11Michael U. Adikwu12Department of Applied Microbiology, Ebonyi State UniversityDepartment of Biological Sciences, Alex Ekwueme Federal University, Ndufu-Alike IkwoDepartment of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical SciencesBiology Department, College of Science, University of BabylonBiology Department, College of Science, University of BabylonDepartment of Medical Microbiology & Parasitology, Nnamdi Azikiwe UniversityDepartment of Applied Microbiology, Ebonyi State UniversityDepartment of Environmental Health Science, Nnamdi Azikiwe UniversityDepartment of Microbiology, Federal University of AgricultureDepartment of Microbiology, Federal UniversityDepartment of Applied Microbiology, Ebonyi State UniversityDepartment of Pharmaceutical Microbiology and Biotechnology, Nnamdi Azikiwe UniversityDepartment of Pharmaceutics, University of NigeriaAbstract Background Gram-negative bacteria (GNB) including Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae represent the most relevant reservoir of resistance genes such as metallo-β-lactamase (MBL) and AmpC genes that give them the undue advantage to resist antimicrobial onslaught. This study aimed to investigate the occurrence of MBL (bla IMP-1, bla IMP-2, bla VIM-1, bla VIM-2) and AmpC (bla FOX, bla DHA, bla CMY, bla ACC) resistance genes in aforementioned GNB collected from abattoir and poultry sources in Nigeria. Results In total, 370 isolates were collected from abattoir tables (n = 130), anal region of cows (n = 120), and the cloacae of poultry birds (n = 120). The test isolates showed high rate of resistance to cephalosporins and carbapenems. The MBLs were phenotypically detected in 22 E. coli, 22 P. aeruginosa, and 18 K. pneumoniae isolates using combined disc test (CDT). However, only 11 E. coli, 24 P. aeruginosa, and 18 Klebsiella pneumoniae isolates were phenotypically confirmed to be AmpC producers using cefoxitin-cloxacillin double disk synergy test (CC-DDST). MBL encoding genes (particularly the bla IMP-1 genes and bla IMP-2 genes) were detected by polymerase chain reaction (PCR) in 12 (54.6%) E. coli, 15 (83.3%) K. pneumoniae, and 16 (72.7%) P. aeruginosa isolates. AmpC genes (particularly the bla CMY genes and bla FOX genes) were found in a total of 5 (29.4%) E. coli isolates, 5 (27.8%) isolates of K. pneumoniae, and 10 (41.7%) isolates of P. aeruginosa. Conclusions Our study showed the circulation of MBL and AmpC genes in GNB from abattoir and poultry origin in Nigeria. Adoption of regular control policies is necessary to reduce the spread of these species as soon as possible, especially in poultry and slaughterhouses.https://doi.org/10.1186/s12866-021-02179-1AmpCAntibiotic resistanceGram-negative bacteriaMetallo-β-lactamaseMultidrug resistanceNigeria |
| spellingShingle | Chika Ejikeugwu Okoro Nworie Morteza Saki Hussein O. M. Al-Dahmoshi Noor S. K. Al-Khafaji Chika Ezeador Emmanuel Nwakaeze Peter Eze Eniola Oni Chidiebere Obi Ifeanyichukwu Iroha Charles Esimone Michael U. Adikwu Metallo-β-lactamase and AmpC genes in Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa isolates from abattoir and poultry origin in Nigeria BMC Microbiology AmpC Antibiotic resistance Gram-negative bacteria Metallo-β-lactamase Multidrug resistance Nigeria |
| title | Metallo-β-lactamase and AmpC genes in Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa isolates from abattoir and poultry origin in Nigeria |
| title_full | Metallo-β-lactamase and AmpC genes in Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa isolates from abattoir and poultry origin in Nigeria |
| title_fullStr | Metallo-β-lactamase and AmpC genes in Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa isolates from abattoir and poultry origin in Nigeria |
| title_full_unstemmed | Metallo-β-lactamase and AmpC genes in Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa isolates from abattoir and poultry origin in Nigeria |
| title_short | Metallo-β-lactamase and AmpC genes in Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa isolates from abattoir and poultry origin in Nigeria |
| title_sort | metallo β lactamase and ampc genes in escherichia coli klebsiella pneumoniae and pseudomonas aeruginosa isolates from abattoir and poultry origin in nigeria |
| topic | AmpC Antibiotic resistance Gram-negative bacteria Metallo-β-lactamase Multidrug resistance Nigeria |
| url | https://doi.org/10.1186/s12866-021-02179-1 |
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