Effects of Bushen Tianjing Recipe in a rat model of tripterygium glycoside-induced premature ovarian failure
Abstract Background Bushen Tianjing Recipe (BTR) is a traditional Chinese herbal medicine that has been prescribed for premature ovarian failure (POF) for decades in China. Nevertheless, little is known regarding its underlying molecular mechanism. In the present study, we investigated the effects o...
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| Format: | Article |
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BMC
2017-04-01
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| Series: | Chinese Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s13020-017-0131-3 |
| _version_ | 1818267336804663296 |
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| author | Xiaofeng Xu Yong Tan Guorong Jiang Xuanyi Chen Rensheng Lai Lurong Zhang Guoqiang Liang |
| author_facet | Xiaofeng Xu Yong Tan Guorong Jiang Xuanyi Chen Rensheng Lai Lurong Zhang Guoqiang Liang |
| author_sort | Xiaofeng Xu |
| collection | DOAJ |
| description | Abstract Background Bushen Tianjing Recipe (BTR) is a traditional Chinese herbal medicine that has been prescribed for premature ovarian failure (POF) for decades in China. Nevertheless, little is known regarding its underlying molecular mechanism. In the present study, we investigated the effects of BTR in a tripterygium glycoside (TG)-induced-POF rat model. Methods Three doses of BTR were administered via intragastric gavage to adult female Sprague–Dawley (SD) rats with TG-induced POF. After 15 days of treatment, the estrous cycle was examined by vaginal smear analysis. Serum levels of estradiol, follicle-stimulating hormone, progesterone, and testosterone were measured by radioimmunoassay. Histological analysis and assessment of apoptosis were performed after hematoxylin and eosin staining of ovarian tissue sections. The expression of vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor 2 (VEGFR2), anti-apoptotic factor Bcl-2, and pro-apoptotic factors Bax and caspase 3 in ovaries of animals was examined by an immunohistochemistry process. Results BTR not only reverted an abnormal estrous cycle and decreased the ovary index in POF rats but also improved the abnormal secretion of reproductive hormones associated with POF. In addition, treatment with BTR can protect ovaries from TG-induced damage, induce intraovarian expression of VEGF and VEGFR2, and regulate intraovarian expression of apoptosis-related proteins. Conclusions Our results show that BTR is effective in the treatment of TG-induced POF rats. Promotion of angiogenesis and anti-apoptosis are most likely to contribute to the effects of BTR against POF. |
| first_indexed | 2024-12-12T20:20:59Z |
| format | Article |
| id | doaj.art-de9a84f888e544d7b671f141be016aae |
| institution | Directory Open Access Journal |
| issn | 1749-8546 |
| language | English |
| last_indexed | 2024-12-12T20:20:59Z |
| publishDate | 2017-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Chinese Medicine |
| spelling | doaj.art-de9a84f888e544d7b671f141be016aae2022-12-22T00:13:16ZengBMCChinese Medicine1749-85462017-04-0112111110.1186/s13020-017-0131-3Effects of Bushen Tianjing Recipe in a rat model of tripterygium glycoside-induced premature ovarian failureXiaofeng Xu0Yong Tan1Guorong Jiang2Xuanyi Chen3Rensheng Lai4Lurong Zhang5Guoqiang Liang6Department of Gynecology, Suzhou Hospital Affiliated to Nanjing University of Chinese MedicineDepartment of Gynecology, The No.1 Clinical Medical College, Nanjing University of Chinese MedicineInstitute of Traditional Chinese Medicine, Suzhou Hospital Affiliated to Nanjing University of Chinese MedicineDepartment of Gynecology, Suzhou Hospital Affiliated to Nanjing University of Chinese MedicineDepartment of Pathology, Jiangsu Hospital of Traditional Chinese MedicineInstitute of Traditional Chinese Medicine, Suzhou Hospital Affiliated to Nanjing University of Chinese MedicineInstitute of Traditional Chinese Medicine, Suzhou Hospital Affiliated to Nanjing University of Chinese MedicineAbstract Background Bushen Tianjing Recipe (BTR) is a traditional Chinese herbal medicine that has been prescribed for premature ovarian failure (POF) for decades in China. Nevertheless, little is known regarding its underlying molecular mechanism. In the present study, we investigated the effects of BTR in a tripterygium glycoside (TG)-induced-POF rat model. Methods Three doses of BTR were administered via intragastric gavage to adult female Sprague–Dawley (SD) rats with TG-induced POF. After 15 days of treatment, the estrous cycle was examined by vaginal smear analysis. Serum levels of estradiol, follicle-stimulating hormone, progesterone, and testosterone were measured by radioimmunoassay. Histological analysis and assessment of apoptosis were performed after hematoxylin and eosin staining of ovarian tissue sections. The expression of vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor 2 (VEGFR2), anti-apoptotic factor Bcl-2, and pro-apoptotic factors Bax and caspase 3 in ovaries of animals was examined by an immunohistochemistry process. Results BTR not only reverted an abnormal estrous cycle and decreased the ovary index in POF rats but also improved the abnormal secretion of reproductive hormones associated with POF. In addition, treatment with BTR can protect ovaries from TG-induced damage, induce intraovarian expression of VEGF and VEGFR2, and regulate intraovarian expression of apoptosis-related proteins. Conclusions Our results show that BTR is effective in the treatment of TG-induced POF rats. Promotion of angiogenesis and anti-apoptosis are most likely to contribute to the effects of BTR against POF.http://link.springer.com/article/10.1186/s13020-017-0131-3Bushen Tianjing RecipePremature ovarian failureTripterygium glycosideAngiogenesisApoptosis |
| spellingShingle | Xiaofeng Xu Yong Tan Guorong Jiang Xuanyi Chen Rensheng Lai Lurong Zhang Guoqiang Liang Effects of Bushen Tianjing Recipe in a rat model of tripterygium glycoside-induced premature ovarian failure Chinese Medicine Bushen Tianjing Recipe Premature ovarian failure Tripterygium glycoside Angiogenesis Apoptosis |
| title | Effects of Bushen Tianjing Recipe in a rat model of tripterygium glycoside-induced premature ovarian failure |
| title_full | Effects of Bushen Tianjing Recipe in a rat model of tripterygium glycoside-induced premature ovarian failure |
| title_fullStr | Effects of Bushen Tianjing Recipe in a rat model of tripterygium glycoside-induced premature ovarian failure |
| title_full_unstemmed | Effects of Bushen Tianjing Recipe in a rat model of tripterygium glycoside-induced premature ovarian failure |
| title_short | Effects of Bushen Tianjing Recipe in a rat model of tripterygium glycoside-induced premature ovarian failure |
| title_sort | effects of bushen tianjing recipe in a rat model of tripterygium glycoside induced premature ovarian failure |
| topic | Bushen Tianjing Recipe Premature ovarian failure Tripterygium glycoside Angiogenesis Apoptosis |
| url | http://link.springer.com/article/10.1186/s13020-017-0131-3 |
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