Optimizing a portable biosensor system for bacterial detection in milk based mix for ice cream
One of the primary focuses of the food industry is providing products compliant with safety standards. The microbiological analysis helps in the identification of the presence of pathogen microorganisms in the food. The analysis with Agar Plate is the classic method. This approach guarantees a high...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-04-01
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| Series: | Sensing and Bio-Sensing Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214180417301460 |
| _version_ | 1818007281099341824 |
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| author | A. Biscotti R. Lazzarini G. Virgilli F. Ngatcha A. Valisi M. Rossi |
| author_facet | A. Biscotti R. Lazzarini G. Virgilli F. Ngatcha A. Valisi M. Rossi |
| author_sort | A. Biscotti |
| collection | DOAJ |
| description | One of the primary focuses of the food industry is providing products compliant with safety standards. The microbiological analysis helps in the identification of the presence of pathogen microorganisms in the food. The analysis with Agar Plate is the classic method. This approach guarantees a high accuracy, but it needs a long detection time (twenty-four to forty-eight hours), beyond high costs and skilled technician. In recent times have been proposed many different methods to have a faster response, and between them there is the impedance method. One of its features is that it is fast, in fact it requires between three to fourteen hours to obtain a reliable measurement. The system is accurate, and suitable to be executed automatically. To test this method has been used UHT Ice Cream Mix. A known volume of mix has been inoculated with increasing percentage of cultures of E. coli. The measurement of the impedance of the inoculated mix has been done by an electronic board designed for the application, and by applying a sinusoidal voltage to the test tube. The signal was digitally generated by the microprocessor, and supplied externally through a D.A. converter. The signal was then filtered to delete from its spectrum the high frequency components typical of the digitally generated signals. The data obtained from impedance instrument showed a reliable correspondence with those from the plate count. By working in less time compared to traditional methods, this tool is well suited for in-situ preliminary analysis in commercial and professional foodservice environment. |
| first_indexed | 2024-04-14T05:14:01Z |
| format | Article |
| id | doaj.art-dea07c7454364ab4bfd04c98c082194a |
| institution | Directory Open Access Journal |
| issn | 2214-1804 |
| language | English |
| last_indexed | 2024-04-14T05:14:01Z |
| publishDate | 2018-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sensing and Bio-Sensing Research |
| spelling | doaj.art-dea07c7454364ab4bfd04c98c082194a2022-12-22T02:10:27ZengElsevierSensing and Bio-Sensing Research2214-18042018-04-0118C1610.1016/j.sbsr.2018.02.002Optimizing a portable biosensor system for bacterial detection in milk based mix for ice creamA. Biscotti0R. Lazzarini1G. Virgilli2F. Ngatcha3A. Valisi4M. Rossi5Carpigiani Ali S.p.A., Anzola Emilia, Bologna, ItalyCarpigiani Ali S.p.A., Anzola Emilia, Bologna, ItalyCarpigiani Ali S.p.A., Anzola Emilia, Bologna, ItalyCarpigiani Ali S.p.A., Anzola Emilia, Bologna, ItalyCarpigiani Ali S.p.A., Anzola Emilia, Bologna, ItalyUniversity of Modena and Reggio Emilia, ItalyOne of the primary focuses of the food industry is providing products compliant with safety standards. The microbiological analysis helps in the identification of the presence of pathogen microorganisms in the food. The analysis with Agar Plate is the classic method. This approach guarantees a high accuracy, but it needs a long detection time (twenty-four to forty-eight hours), beyond high costs and skilled technician. In recent times have been proposed many different methods to have a faster response, and between them there is the impedance method. One of its features is that it is fast, in fact it requires between three to fourteen hours to obtain a reliable measurement. The system is accurate, and suitable to be executed automatically. To test this method has been used UHT Ice Cream Mix. A known volume of mix has been inoculated with increasing percentage of cultures of E. coli. The measurement of the impedance of the inoculated mix has been done by an electronic board designed for the application, and by applying a sinusoidal voltage to the test tube. The signal was digitally generated by the microprocessor, and supplied externally through a D.A. converter. The signal was then filtered to delete from its spectrum the high frequency components typical of the digitally generated signals. The data obtained from impedance instrument showed a reliable correspondence with those from the plate count. By working in less time compared to traditional methods, this tool is well suited for in-situ preliminary analysis in commercial and professional foodservice environment.http://www.sciencedirect.com/science/article/pii/S2214180417301460BiosensorMilk based mix for ice creamIce creamImpedance measurementsPhase shift |
| spellingShingle | A. Biscotti R. Lazzarini G. Virgilli F. Ngatcha A. Valisi M. Rossi Optimizing a portable biosensor system for bacterial detection in milk based mix for ice cream Sensing and Bio-Sensing Research Biosensor Milk based mix for ice cream Ice cream Impedance measurements Phase shift |
| title | Optimizing a portable biosensor system for bacterial detection in milk based mix for ice cream |
| title_full | Optimizing a portable biosensor system for bacterial detection in milk based mix for ice cream |
| title_fullStr | Optimizing a portable biosensor system for bacterial detection in milk based mix for ice cream |
| title_full_unstemmed | Optimizing a portable biosensor system for bacterial detection in milk based mix for ice cream |
| title_short | Optimizing a portable biosensor system for bacterial detection in milk based mix for ice cream |
| title_sort | optimizing a portable biosensor system for bacterial detection in milk based mix for ice cream |
| topic | Biosensor Milk based mix for ice cream Ice cream Impedance measurements Phase shift |
| url | http://www.sciencedirect.com/science/article/pii/S2214180417301460 |
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