Alterations in gut microbiota of abdominal aortic aneurysm mice
Abstract Background The gut microbiome plays an important role in various cardiovascular diseases, such as atherosclerosis and hypertension, which are associated with abdominal aortic aneurysms (AAAs). Methods Here, we used 16S rRNA sequencing to explore gut microbiota in C57BL ApoE−/− mice with AAA...
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| Format: | Article |
| Language: | English |
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BMC
2020-01-01
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| Series: | BMC Cardiovascular Disorders |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12872-020-01334-2 |
| _version_ | 1818460948299513856 |
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| author | Jiahe Xie Weiling Lu Lintao Zhong Yuhua Hu Qingrui Li Rongming Ding Zhonggao Zhong Ziyou Liu Hai Xiao Dongming Xie Guofu Zheng Bo Ye Yiming Zhong Zuheng Liu |
| author_facet | Jiahe Xie Weiling Lu Lintao Zhong Yuhua Hu Qingrui Li Rongming Ding Zhonggao Zhong Ziyou Liu Hai Xiao Dongming Xie Guofu Zheng Bo Ye Yiming Zhong Zuheng Liu |
| author_sort | Jiahe Xie |
| collection | DOAJ |
| description | Abstract Background The gut microbiome plays an important role in various cardiovascular diseases, such as atherosclerosis and hypertension, which are associated with abdominal aortic aneurysms (AAAs). Methods Here, we used 16S rRNA sequencing to explore gut microbiota in C57BL ApoE−/− mice with AAAs. A mouse model of abdominal aortic aneurysms was induced with angiotensin II (Ang II) (1000 ng/min per kg). On day 28 after the operation, fecal samples were collected and stored at − 80 °C until DNA extraction. We determined the relative abundances of bacterial taxonomic groups using 16S rRNA amplicon metabarcoding, and sequences were analyzed using a combination of mother software and UPARSE. Results We found that the gut microbiome was different between control and AAA mice. The results of correlation analysis between AAA diameter and the gut microbiome as well as LEfSe of the genera Akkermansia, Odoribacter, Helicobacter and Ruminococcus might be important in the progression of AAAs. Conclusions AAA mice is subjected to gut microbial dysbiosis, and gut microbiota might be a potential target for further investigation. |
| first_indexed | 2024-12-14T23:38:21Z |
| format | Article |
| id | doaj.art-dea26695bc0c46a6aed09e372ece88f7 |
| institution | Directory Open Access Journal |
| issn | 1471-2261 |
| language | English |
| last_indexed | 2024-12-14T23:38:21Z |
| publishDate | 2020-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cardiovascular Disorders |
| spelling | doaj.art-dea26695bc0c46a6aed09e372ece88f72022-12-21T22:43:35ZengBMCBMC Cardiovascular Disorders1471-22612020-01-012011710.1186/s12872-020-01334-2Alterations in gut microbiota of abdominal aortic aneurysm miceJiahe Xie0Weiling Lu1Lintao Zhong2Yuhua Hu3Qingrui Li4Rongming Ding5Zhonggao Zhong6Ziyou Liu7Hai Xiao8Dongming Xie9Guofu Zheng10Bo Ye11Yiming Zhong12Zuheng Liu13Department of Cardiology, First Affiliated Hospital of Gannan Medical UniversityDepartment of Cardiology, First Affiliated Hospital of Gannan Medical UniversityDepartment of Cardiology, Zhuhai Hospital, Jinan UniversityDepartment of Cardiology, First Affiliated Hospital of Gannan Medical UniversityDepartment of Cardiology, First Affiliated Hospital of Gannan Medical UniversityDepartment of Cardiology, First Affiliated Hospital of Gannan Medical UniversityDepartment of Cardiology, First Affiliated Hospital of Gannan Medical UniversityKey Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases, Ministry of Education, Gannan Medical UniversityKey Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases, Ministry of Education, Gannan Medical UniversityDepartment of Cardiology, First Affiliated Hospital of Gannan Medical UniversityDepartment of Vascular Surgery, the Affiliated Ganzhou Hospital of Nanchang UniversityDepartment of Vascular Surgery, the Affiliated Ganzhou Hospital of Nanchang UniversityDepartment of Cardiology, First Affiliated Hospital of Gannan Medical UniversityState Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang Hospital, Southern Medical UniversityAbstract Background The gut microbiome plays an important role in various cardiovascular diseases, such as atherosclerosis and hypertension, which are associated with abdominal aortic aneurysms (AAAs). Methods Here, we used 16S rRNA sequencing to explore gut microbiota in C57BL ApoE−/− mice with AAAs. A mouse model of abdominal aortic aneurysms was induced with angiotensin II (Ang II) (1000 ng/min per kg). On day 28 after the operation, fecal samples were collected and stored at − 80 °C until DNA extraction. We determined the relative abundances of bacterial taxonomic groups using 16S rRNA amplicon metabarcoding, and sequences were analyzed using a combination of mother software and UPARSE. Results We found that the gut microbiome was different between control and AAA mice. The results of correlation analysis between AAA diameter and the gut microbiome as well as LEfSe of the genera Akkermansia, Odoribacter, Helicobacter and Ruminococcus might be important in the progression of AAAs. Conclusions AAA mice is subjected to gut microbial dysbiosis, and gut microbiota might be a potential target for further investigation.https://doi.org/10.1186/s12872-020-01334-2Abdominal aortic aneurysmGut microbiotaAkkermansia |
| spellingShingle | Jiahe Xie Weiling Lu Lintao Zhong Yuhua Hu Qingrui Li Rongming Ding Zhonggao Zhong Ziyou Liu Hai Xiao Dongming Xie Guofu Zheng Bo Ye Yiming Zhong Zuheng Liu Alterations in gut microbiota of abdominal aortic aneurysm mice BMC Cardiovascular Disorders Abdominal aortic aneurysm Gut microbiota Akkermansia |
| title | Alterations in gut microbiota of abdominal aortic aneurysm mice |
| title_full | Alterations in gut microbiota of abdominal aortic aneurysm mice |
| title_fullStr | Alterations in gut microbiota of abdominal aortic aneurysm mice |
| title_full_unstemmed | Alterations in gut microbiota of abdominal aortic aneurysm mice |
| title_short | Alterations in gut microbiota of abdominal aortic aneurysm mice |
| title_sort | alterations in gut microbiota of abdominal aortic aneurysm mice |
| topic | Abdominal aortic aneurysm Gut microbiota Akkermansia |
| url | https://doi.org/10.1186/s12872-020-01334-2 |
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